Poly-extremophiles: exploring the limits of habitability

Brevibacterium linens AE038-8 is an arsenic hyper-tolerant bacterial strain, previously isolated from well water in Tucumán, Argentina. The aim of this study is to investigate the resistance of this strain to different stress factors relevant for astrobiology studies. We found that B. linens AE038-8 is capable of tolerating high concentrations of heavy metals such as Cd(II), Cr(VI) and Cu(II), and a range of temperatures from 10 to 30°C. It is also able to grow in the range of pH values from 3 to 11, and when grown in the presence of NaCl, it can tolerate concentrations up to 5 M. We also study the ability of the strain to grow under combined stress conditions. In particular, we found that it is able to grow under high NaCl concentrations and low pH, conditions relevant to the habitability of brines in Mars.Fil: Maizel, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Astronomía y Física del Espacio. - Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Astronomía y Física del Espacio; ArgentinaFil: Alche, Laura Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Mauas, Pablo Jacobo David. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Astronomía y Física del Espacio. - Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Astronomía y Física del Espacio; Argentin

First long-term activity study of AU Microscopii: a possible chromospheric cycle

M stars are ideal targets to search for Earth-like planets. However, they usually have high levels of magnetic activity, which could affect their habitability and make difficult the detection of exoplanets orbiting around them. Unfortunately, long-term variability of dM stars has not been extensively studied, due to their low intrinsic brightness. For this reason, in 1999 we started the HKα project, which systematically observes the spectra of a large number of stars, in particular dM stars, at the Complejo Astronómico El Leoncito (CASLEO). In this work, we study the long-term activity of the young active dM1 star AU Microscopii. We analyse the Mount Wilson index S derived from CASLEO spectra obtained between 2004 and 2016, which we complement with the S-index derived from HARPS, FEROS, and UVES public spectra. We also analyse the simultaneous photometric counterpart provided by the ASAS public data base for this star between 2000 and 2009, and our own photometry. In both totally independent time series, we detect a possible activity cycle of period ∼5 yr. We also derived a precise rotation period for this star Prot = 4.85 d, consistent with the literature. This activity cycle reflects that an αΩ dynamo could be operating in this star.Fil: Ibañez Bustos, Romina Valeria. Consejo Nacional de Investigaciónes Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Astronomía y Física del Espacio. - Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Astronomía y Física del Espacio; ArgentinaFil: Buccino, Andrea Paola. Consejo Nacional de Investigaciónes Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Astronomía y Física del Espacio. - Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Astronomía y Física del Espacio; ArgentinaFil: Flores, M.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan. Instituto de Ciencias Astronómicas, de la Tierra y del Espacio. Universidad Nacional de San Juan. Instituto de Ciencias Astronómicas, de la Tierra y del Espacio; Argentina. Universidad Nacional de San Juan. Facultad de Ciencias Exactas, Físicas y Naturales; ArgentinaFil: Martinez, Cristian Israel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan. Instituto de Ciencias Astronómicas, de la Tierra y del Espacio. Universidad Nacional de San Juan. Instituto de Ciencias Astronómicas, de la Tierra y del Espacio; ArgentinaFil: Maizel, Daniela. Consejo Nacional de Investigaciónes Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Astronomía y Física del Espacio. - Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Astronomía y Física del Espacio; ArgentinaFil: Messina, Sergio. Catania Astrophysical Observatory; ItaliaFil: Mauas, Pablo Jacobo David. Consejo Nacional de Investigaciónes Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Astronomía y Física del Espacio. - Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Astronomía y Física del Espacio; Argentin

Filtration efficiency of air conditioner filters and face masks to limit exposure to aerosolized algal toxins

Harmful algal blooms (HABs) can generate toxins that can be aerosolized and negatively impact human health through inhalation. HABs are often found in waterways near residences, therefore, aerosolized HAB toxins can potentially affect both indoor and outdoor air quality. Given that HABs are predicted to increase worldwide, effective mitigation strategies are needed to prevent the inhalation of aerosolized HAB toxins. In this work, we characterized both the particle filtration efficiency using particle sizing instruments as well as the mass concentration of different congeners of aerosolized microcystin (MC) toxins that penetrate through commercially available face masks and air conditioner (AC) filters. Particles were generated from cultures of the toxin-producing cyanobacteria Microcystis aeruginosa. Hydrophobic congeners of microcystin including MC-LF and MC-LW were enriched in aerosols compared to water, with MC-LR being the most abundant, which has implications for the toxicity of inhalable particles generated from HAB-contaminated waters. Particle transmission efficiencies and toxin filtration efficiencies scaled with the manufacturer-provided filter performance ratings. Up to 80% of small, microcystin-containing aerosols were transmitted through AC filters with low filter performance ratings. In contrast, both face masks as well as AC filters with high filter performance ratings efficiently removed toxin-containing particles to below limits of quantification. Our findings suggest that face masks and commercially available AC filters with high filtration efficiency ratings are suitable mitigation strategies to avoid indoor and outdoor air exposure to aerosolized HAB toxins. This work also has relevance for reducing airborne exposure to other HAB toxins, non-HAB toxins, pathogens, and viruses, including SARS-CoV-2, the virus responsible for the COVID-19 pandemic

Indigenous PAH-degrading bacteria in oil-polluted marine sediments from Patagonia: Diversity and biotechnological properties

The diversity of indigenous bacteria recovered from polluted sediment samples from several coasts of Patagonia is described in this chapter. Selective enrichment cultures supplemented with naphthalene, phenanthrene, and pyrene allowed isolation of bacteria with the capability to degrade polycyclic aromatic hydrocarbons (PAHs). Bacterial communities of different composition (analyzed by denaturing gradient gel electrophoresis, DGGE) showed changes along with enrichment culture conditions. The ability of isolates to grow and remove different low and high molecular weight PAHs was demonstrated by detection of the residual substrate by HPLC. The presence and differential expression of naphthalene and catechol dioxygenase genes in several isolates suggest biodegradation potential in these sediments. Successful bacterial isolation with the ability to degrade PAH in pure and mixed cultures allows discussing the possibility to study and to further consider strategies to increase the intrinsic bioremediation opportunities on polluted coasts of Patagonia. Other biotechnological properties are also considered in this chapter, such as biosurfactant production and other biotransformations.Fil: Isaac, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones y Transferencia de Villa María. Universidad Nacional de Villa María. Centro de Investigaciones y Transferencia de Villa María; ArgentinaFil: Bourguignon, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Maizel, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Ferrero, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentin

An arsenic resistant bacteria isolated in Tucumán, Argentina, as a model microorganism for astrobiology studies.

Astrobiology can be defined as a multidisciplinary science that focuses on studies about the origin, evolution, distribution and future of life in the Universe (https://astrobiology. nasa.gov/nai/).In the last 20 years, studies of extrasolar planets have progressed considerably, focusing on the search of planets similar to Earth and in the Habitability Zone (HZ), with a major interest in the search of evidence of life in such environments. It is readily evident that our notion of ?habitability? relies on our limited knowledge of life on Earth. Therefore, in an attempt to find possible forms of life in extraterrestrial systems, it becomes fundamental to enlarge our knowledge about extreme life forms inside our own planet. Such forms of life able to tolerate extreme conditions are mostly known as ?extremophiles? (Caviccioli 2002; Das Sarma, 2006). Some of these organisms have been recently proposed as models for astrobiology studies (Abrevaya et al, 2010; 2011).An interesting case of extremophiles is represented by arsenic- resistant bacteria. Arsenic is a toxic metalloid widely spread in nature. It generally occurs as either arsenate [HAsO42- or As(V)] or arsenite [H2AsO3 or As(III)], the latter species being more toxic than the former. It can be released either by natural weathering of rocks or by anthropogenic sources (Muller et al., 2003). Arsenic is toxic because As and P are similar enough that living organisms attempt this substitution (Wolfe-Simon et al, 2009). Although arsenic is toxic to almost every form of life, it has been previously demonstrated that microorganisms can resist it and also utilize arsenic compounds as a source for growth (Krumova et al., 2008). It has been previously suggested that arsenic-based forms of life could be present on Earth within a ?shadow biosphere?, in similar environments to the ones found outside of the Earth (Davies et al, 2009). These alternative forms of life could currently exist in arsenic-rich environments as much as inside Earth itself as in extraterrestrial systems (Cleland & Copley, 2005; Davies et al, 2009). Even though all known life requires phosphorus (P) in the form of inorganic phosphate, In 2010 Wolfe-Simon et al. reported on a bacterial strain isolated from Mono Lake (California, USA) known as GFAJ-1, which is able to survive in high arsenic concentrations and in the absence of phosphorus. The authors of the work suggested GFAJ-1 as a habitability model in other planets, where different forms of life could be based on arsenic (Wolfe-Simon et al, 2011).Brevibacterium linens AE038-8, is a bacterial strain isolated from As-contaminated groundwater in Tucumán (Argentina), highly resistant to arsenic compounds and capable of growing in extremely low phosphate concentrations, showing a physiology comparable to that of GFAJ-1. It?s recently sequenced genome (Maizel et al, 2015) revealed the presence of arsenate reductase enzymes previously described, which might have evolved from arsenic-rich environments in early life on Earth (Wolfe-Simon et al, 2009). Therefore, we propose strain AE038-8 as an interesting prospect for studies of life in extrasolar planets.Fil: Maizel, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Ferrero, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Mauas, Pablo Jacobo David. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Astronomía y Física del Espacio. - Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Astronomía y Física del Espacio; Argentina58a Reunión Anual de la Asociación Argentina de AstronomíaLa PlataArgentinaUniversidad Nacional de La Plata. Facultad de Ciencias Astronómicas y GeofísicasUniversidad Nacional de La Plata. Instituto de Astrofíısica de La Plat

Draft Genome Sequence of Brevibacterium linens AE038-8, an Extremely Arsenic-Resistant Bacterium

To understand the arsenic biogeocycles in the groundwaters at Tucumán, Argentina, we isolated Brevibacterium linens sp. strain AE38-8, obtained from arsenic-contaminated well water. This strain is extremely resistant to arsenicals and has arsenic resistance (ars) genes in its genome. Here, we report the draft genome sequence of B. linens AE38-8.Fil: Maizel, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; ArgentinaFil: Utturkar, Sagar M.. University Of Tennessee, Estados Unidos;Fil: Brown, Steven D.. Oak Ridge National Laboratory, Estados Unidos;Fil: Ferrero, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; ArgentinaFil: Rosen, Barry. Herbert Wertheim College Of Medicine, Fiu, Eeuu

Arsenic-hypertolerant and arsenic-reducing bacteria isolated from wells in Tucumán, Argentina

Arsenic-hypertolerant bacteria were isolated from arsenic-contaminated well water from the village of Los Pereyra in Tucumán province, Argentina. Microorganisms that biotransform arsenic are a major factor in arsenic mobilization in contaminated aquifers. Groundwater analyses showed a level of arsenic contamination (mean concentration of 978 μg·L−1) that exceeds the safe drinking water limit of 10 μg·L−1 recommended by the World Health Organization and the Argentine Food Code. There was considerable spatial variability in the concentration of arsenic in each of the wells analyzed and in the distribution of the major anions HCO3–, SO42–, and Cl–. Eighteen bacterial strains were characterized. Six strains belonging to the Actinobacteria phylum were able to grow in media with 20 mmol·L–1 As(III) or 200 mmol·L–1 As(V) and were also highly resistant to Cr, Cd, and Cu. Their ability to biotransform arsenic was examined by speciation of the products by high-performance liquid chromatography inductively coupled plasma mass spectrometry. In addition, two strains, Brevibacterium sp. strain AE038-4 and Microbacterium sp. strain AE038-20, were capable of aerobic arsenate reduction, which suggests that these strains could increase the mobility of arsenic by formation of more mobile As(III).Des bactéries hypertolérantes à l’arsenic ont été isolées de l’eau de puits contaminés à l’arsenic du village de Los Pereyra dans la province de Tucumán, en Argentine. Les microorganismes qui biotransforment l’arsenic constituent un facteur clé de la mobilisation d’arsenic dans les aquifères contaminés. Des analyses de la nappe phréatique ont montré un niveau de contamination à l’arsenic (concentration moyenne de 978 μg·L–1) qui outrepasse la limite sécuritaire dans l’eau potable de 10 μg·L–1 recommandée par l’Organisation mondiale de la santé et le code alimentaire de l’Argentine. Il existait une variabilité spatiale considérable dans la concentration d’arsenic de chacun des puits analysés, de même que dans la distribution des principaux anions HCO3–, SO42– et Cl–. Dix-huit souches bactériennes ont été caractérisées. Six souches appartenant au phylum des Actinobacteria étaient capables de croître dans des milieux contenant 20 mmol·L–1 As(III) ou 200 mmol·L–1 As(V), et elles étaient hautement résistantes au Cr, au Cd et au Cu. Leur capacité à biotransformer l’arsenic a été examinée par la spéciation des produits par chromatographie liquide à haute performance et spectrométrie de masse avec plasma à couplage inductif. Deux souches, Brevibacterium sp. souche AE038-4 et Microbacterium sp. souche AE038-20, étaient par ailleurs capables de réduire l’arséniate en aérobie, ce qui suggère que ces souches pourraient accroître la mobilité de l’arsenic par la formation d’As(III) plus mobile.Fil: Maizel, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Astronomía y Física del Espacio; ArgentinaFil: Balverdi, María del Pilar. Universidad Nacional de Tucuman. Facultad de Bioquímica, Química y Farmacia. Instituto de Química Analitica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rosen, Barry. Florida International University; Estados UnidosFil: Sales, Adriana María. Universidad Nacional de Tucuman. Facultad de Bioquímica, Química y Farmacia. Instituto de Química Analitica; ArgentinaFil: Ferrero, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán; Argentin

Identification of proteins induced by polycyclic aromatic hydrocarbon and proposal of the phenanthrene catabolic pathway in Amycolatopsis tucumanensis DSM 45259

In the present study the polycyclic aromatic hydrocarbon removal and metabolic adaptation of Amycolatopsis tucumanensis DSM 45259 were investigated. Analysis of one-dimensional gel electrophoresis of crude cell extracts revealed differential synthesis of proteins which were identified by MALDI-TOF. To elucidate the phenanthrene metabolic pathway in A. tucumanensis DSM45259, two-dimensional electrophoresis and detection of phenanthrene degradation intermediates by GS-MS were performed. The presence of aromatic substrates resulted in changes in the abundance of proteins involved in the metabolism of aromatic compounds, oxidative stress response, energy production and protein synthesis. The obtained results allowed us to clarify the phenanthrene catabolic pathway, by confirming the roles of several proteins involved in the degradation process and comprehensive adaptation. This may clear the way for more efficient engineering of bacteria in the direction of more effective bioremediation applications.Fil: Bourguignon, Natalia. Universidad Tecnológica Nacional. Facultad Regional Haedo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Irazusta, Verónica Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina. Universidad Nacional de Salta. Facultad de Ciencias Naturales. Instituto Geonorte; ArgentinaFil: Isaac, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones y Transferencia de Villa María. Universidad Nacional de Villa María. Centro de Investigaciones y Transferencia de Villa María; Argentina. Universidad Nacional de Villa María. Instituto Académico Pedagógico de Ciencias Básicas y Aplicadas; ArgentinaFil: Estévez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Maizel, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Astronomía y Física del Espacio. - Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Astronomía y Física del Espacio; ArgentinaFil: Ferrero, Marcela Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentin

Characterization of the extremely arsenic-resistant Brevibacterium linens strain AE038-8 isolated from contaminated groundwater in Tucumán, Argentina

Brevibacterium linens AE038-8, isolated from As-contaminated groundwater in Tucum an (Argentina), is highly resistant to arsenic oxyanions, being able to tolerate up to 1 M As(V) and 75 mM As(III) in a complex medium. Strain AE038-8 was also able to reduce As(V) to As(III) when grown in complex medium but paradoxically it could not do this in a defined minimal medium with sodium acetate and ammonium sulfate as carbon and nitrogen sources, respectively. No oxidation of As(III) to As(V) was observed under any conditions. Three copies of the ars operon comprising arsenic resistance genes were found on B. linens AE038-8 genome. In addition to the well known arsC, ACR3 and arsR, two copies of the arsO gene of unknown function were detected.Fil: Maizel, Daniela. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciónes Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Astronomía y Física del Espacio. - Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Astronomía y Física del Espacio; ArgentinaFil: Blum, Jodi Switzer. United States Geological Survey; Estados UnidosFil: Ferrero, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Utturkar, Sagar M.. The University Of Tennessee System; Estados UnidosFil: Brown, Steven D.. University of Tennessee; Estados UnidosFil: Rosen, Barry. Florida International University; Estados UnidosFil: Oremland, Ronald S.. United States Geological Survey; Estados Unido

Study of the Extremely-Tolerant Brevibacterium linens AE038-8 with Antiviral Activity Against Herpes Simplex Virus Type 1

Brevibacterium linens AE038-8 is an arsenic hyper-tolerant bacterial strain, previously isolated from well water in Tucumán, Argentina. The aim of this study was to characterize this strain regarding its resistance to different stress factors and to evaluate its antiviral activity against Herpes simplex virus type 1 (HSV-1). We found that B. linens AE038-8 was capable of tolerating high concentrations of heavy metals such as Cd(II), Cr(VI) and Cu(II). When grown in the presence of NaCl, it could tolerate up to 3 M in LB25 medium. When cultivated, B. linens released to the supernatants a bioactive principle with antiviral activity against HSV-1 virus regardless growth conditions.Fil: Maizel, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciónes Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Astronomía y Física del Espacio. - Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Astronomía y Física del Espacio; ArgentinaFil: Salinas, Franco Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Solórzano, Inés. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; ArgentinaFil: Raiger Iustman, Laura Judith. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Ferrero, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Mauas, Pablo Jacobo David. Consejo Nacional de Investigaciónes Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Astronomía y Física del Espacio. - Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Astronomía y Física del Espacio; ArgentinaFil: Alche, Laura Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentin