34 research outputs found

    Implementing Archaeological Conservation During American Nation-Building Efforts

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    This thesis seeks to define best practices for implementing the conservation of archaeological sites as part of a broader system of cultural heritage protection within the framework of United States nation-building efforts. The ransacking of the Baghdad Museum, plus the widespread looting of the Iraq’s archaeological sites, makes it clear that measures for cultural property protection within the United States government military framework deserve a critical analysis. First, the importance of protecting cultural property during armed conflict will be examined from a historical and military perspective. Next, previous American nation building attempts are discussed to give a sense of the general circumstances within which conservation activities are to be conducted. Specifically, Iraq will be analyzed as a prime example of the necessity of cultural heritage protection and the damage that can be inflicted on archaeological heritage when such protection is not included as part of larger operational planning framework. Then, what the United States has done and is currently doing in response to the ratification of the Hague Convention and the destruction of cultural property in Iraq are explored. After that, internationally-accepted best practices of archaeological conservation are provided as a framework for evaluating current endeavors and planning those for the future. Finally, recommendations will be made on how the government, specifically the Department of Defense and the State Department, can institute measures for the conservation of archaeological heritage during the planning process of nation building operations

    Three-dimensional Ultrasound in Horses

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    Significance of Physiological Aortic Regurgitation With Coexisting Disease in the Horse

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    A Study of Endothelin Release From Equine Endocardial Cells

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    Three-dimensional Ultrasonography of Equine Tendons

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    Comparison of Ca2+ release and uptake characteristics of the sarcoplasmic reticulum in isolated horse and rabbit cardiomyocytes

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    Both the cardiac action potential duration (APD) (0.6–1 s) and resting heart rate (30–40 beats/min) in the horse are significantly different from humans and smaller mammals, including the rabbit. This would be anticipated to have consequences for excitation-contraction (EC) coupling and require adaptation of the individual processes involved. The sarcoplasmic reticulum (SR) is one of the main components involved in EC coupling. This study examines and compares the activity of this organelle in the horse with that of the rabbit. In particular, the study focuses on SR Ca2+ release via the Ca2+ release channel/ryanodine receptor (RyR2) and Ca2+ uptake via the sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) pump. Isolated cardiomyocytes from both horse and rabbit hearts were permeabilized, bathed in a mock intracellular solution, and exposed to a specified [Ca2+]. Rabbit cardiomyocytes exposed to 260 nM [Ca2+] produced spontaneous Ca2+ release and propagated Ca2+ waves. Horse cells failed to produce Ca2+ waves; instead, only local release in the form of Ca2+ sparks was evident. However, at 550 nM [Ca2+], Ca2+ waves were produced in both species. Ca2+ waves were four times less frequent yet ~1.5 times greater in amplitude in the horse compared with the rabbit. Ca2+ wave velocity was comparable between the species. The reason for this disparity in Ca2+ wave characteristics is unknown. Separate measurements of oxalate-supported Ca2+ uptake into the SR suggest that both horse and rabbit cardiomyocytes have comparable levels SERCA activity. The possible reasons for the observed differences in SR Ca2+ release between the horse and rabbit are discussed

    Methods for the isolation, culture and characterisation of equine pulmonary artery endothelial cells

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    Equine endothelial cells were isolated from the pulmonary artery by enzymatic digestion and grown to confluency. The cells were characterised by positive immunofluorescent staining for von Willebrand factor and NADPH-diaphorase staining for nitric oxide synthase. Measurements of endothelins indicated that there were significant release rates from the cells for up to six hours. Measurements of intracellular calcium concentration showed that the application of bradykinin caused a transient increase in calcium concentration with similar characteristics to those observed in other endothelial cell preparations. These tests verify the endothelial character of these cells and establish the method as a reliable means of producing a primary culture of equine endothelial cells

    The Role of Endothelins in Equine Pulmonary Arteries in Vitro

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