70 research outputs found
Block of c-myc expression by antisense oligonucleotides inhibits proliferation of human thyroid carcinoma cell lines
Expression of high-mobility-group-protein HMGI-C mRNA in the peripheral blood is an independent poor prognostic indicator for survival in metastatic breast cancer
UBE2C (ubiquitin-conjugating enzyme E2C)
Review on UBE2C (ubiquitin-conjugating enzyme E2C), with data on DNA, on the protein encoded, and where the gene is implicated
Immune response in mice and effects on cells by outer membrane porins from Salmonella typhimurium
THE ADENOVIRUS E1A GENE BLOCKS THE DIFFERENTIATION OF A THYROID EPITHELIAL-CELL LINE, HOWEVER THE NEOPLASTIC PHENOTYPE IS ACHIEVED ONLY AFTER COOPERATION WITH OTHER ONCOGENES
The PC Cl 3 cell line is a well characterized epithelial thyroid cell line of Fischer rat origin. This cell line has the peculiarity of retaining in vitro the typical markers of thyroid differentiation (i.e. thyroglobulin synthesis and secretion, iodide uptake and dependence on TSH for growth). The PC Cl 3 cells have been transfected with the E1A gene of Adenovirus 5. The E1A transfected cells, PC E1A, partially lost the dependency on TSH for growth and completely lost the ability to trap iodide and synthesize thyroglobulin; however they did not acquire the typical markers of the neoplastic phenotype. A highly malignant phenotype was achieved after infection of the PC E1A cells with retroviruses carrying the v-raf, v-abl and polyoma virus middle T oncogenes. In contrast, the PC E1A cells transfected with the E1B gene of Adenovirus were not tumorigenic at all, and those infected with retroviruses carrying oncogenes of the ras family displayed a very weak tumorigenic phenotype
One- and two-step transformations of rat thyroid epithelial cells by retroviral oncogenes.
A system of epithelial cells is described in which it is possible to study the number and the nature of genes capable of conferring the malignant phenotype. Two fully differentiated, hormone-responsive cell lines from rat thyroid glands are presented which are susceptible to one-step or two-step transformation upon infection with several murine acute retroviruses. After infection, both cell lines became independent from their thyrotropic hormone requirement for growth. However, complete transformation was achieved with one of the cell lines (FRTL-5 Cl 2), whereas the other cell line (PC Cl 3) failed to grow in agar and to give rise to tumors in vivo. The latter cell line was susceptible to complete transformation upon cooperation of the v-ras-Ha and the human c-myc oncogenes
Mitogenic and dedifferentiating effect of the K-fgf/hst oncogene on rat thyroid PC clone 3 epithelial cells.
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