Guided Lock of a Suspended Optical Cavity Enhanced by a Higher Order Extrapolation

Lock acquisition of a suspended optical cavity can be a highly stochastic process and is therefore nontrivial. Guided lock is a method to make lock acquisition less stochastic by decelerating the motion of the cavity length based on an extrapolation of the motion from an instantaneous velocity measurement. We propose an improved scheme which is less susceptible to seismic disturbances by incorporating the acceleration as a higher order correction in the extrapolation. We implemented the new scheme in a 300-m suspended Fabry-Perot cavity and improved the success rate of lock acquisition by a factor of 30

Neural Activity Changes Underlying the Working Memory Deficit in Alpha-CaMKII Heterozygous Knockout Mice

The alpha-isoform of calcium/calmodulin-dependent protein kinase II (α-CaMKII) is expressed abundantly in the forebrain and is considered to have an essential role in synaptic plasticity and cognitive function. Previously, we reported that mice heterozygous for a null mutation of α-CaMKII (α-CaMKII+/−) have profoundly dysregulated behaviors including a severe working memory deficit, which is an endophenotype of schizophrenia and other psychiatric disorders. In addition, we found that almost all the neurons in the dentate gyrus (DG) of the mutant mice failed to mature at molecular, morphological and electrophysiological levels. In the present study, to identify the brain substrates of the working memory deficit in the mutant mice, we examined the expression of the immediate early genes (IEGs), c-Fos and Arc, in the brain after a working memory version of the eight-arm radial maze test. c-Fos expression was abolished almost completely in the DG and was reduced significantly in neurons in the CA1 and CA3 areas of the hippocampus, central amygdala, and medial prefrontal cortex (mPFC). However, c-Fos expression was intact in the entorhinal and visual cortices. Immunohistochemical studies using arc promoter driven dVenus transgenic mice demonstrated that arc gene activation after the working memory task occurred in mature, but not immature neurons in the DG of wild-type mice. These results suggest crucial insights for the neural circuits underlying spatial mnemonic processing during a working memory task and suggest the involvement of α-CaMKII in the proper maturation and integration of DG neurons into these circuits

Fluoxetine-induced dematuration of hippocampal neurons and adult cortical neurogenesis in the common marmoset

The selective serotonin reuptake inhibitor fluoxetine (FLX) is widely used to treat depression and anxiety disorders. Chronic FLX treatment reportedly induces cellular responses in the brain, including increased adult hippocampal and cortical neurogenesis and reversal of neuron maturation in the hippocampus, amygdala, and cortex. However, because most previous studies have used rodent models, it remains unclear whether these FLX-induced changes occur in the primate brain. To evaluate the effects of FLX in the primate brain, we used immunohistological methods to assess neurogenesis and the expression of neuronal maturity markers following chronic FLX treatment (3 mg/kg/day for 4 weeks) in adult marmosets (n = 3 per group). We found increased expression of doublecortin and calretinin, markers of immature neurons, in the hippocampal dentate gyrus of FLX-treated marmosets. Further, FLX treatment reduced parvalbumin expression and the number of neurons with perineuronal nets, which indicate mature fast-spiking interneurons, in the hippocampus, but not in the amygdala or cerebral cortex. We also found that FLX treatment increased the generation of cortical interneurons; however, significant up-regulation of adult hippocampal neurogenesis was not observed in FLX-treated marmosets. These results suggest that dematuration of hippocampal neurons and increased cortical neurogenesis may play roles in FLX-induced effects and/or side effects. Our results are consistent with those of previous studies showing hippocampal dematuration and increased cortical neurogenesis in FLX-treated rodents. In contrast, FLX did not affect hippocampal neurogenesis or dematuration of interneurons in the amygdala and cerebral cortex

Development of a frequency-detuned interferometer as a prototype experiment for next-generation gravitational-wave detectors

We report on our prototype experiment that uses a 4-m detuned resonant sideband extraction interferometer with suspended mirrors, which has almost the same configuration as the next-generation, gravitational-wave detectors. We have developed a new control scheme and have succeeded in the operation of such an interferometer with suspended mirrors for the first time ever as far as we know. We believe that this is the first such instrument that can see the radiation pressure signal enhancement, which can improve the sensitivity of next-generation gravitational-wave detectors

Expression of progenitor cell/immature neuron markers does not present definitive evidence for adult neurogenesis

It is agreed upon that adult hippocampal neurogenesis (AHN) occurs in the dentate gyrus (DG) in rodents. However, the existence of AHN in humans, particularly in elderly individuals, remains to be determined. Recently, several studies reported that neural progenitor cells, neuroblasts, and immature neurons were detected in the hippocampus of elderly humans, based on the expressions of putative markers for these cells, claiming that this provides evidence of the persistence of AHN in humans. Herein, we briefly overview the phenomenon that we call “dematuration, ” in which mature neurons dedifferentiate to a pseudo-immature status and re-express the molecular markers of neural progenitor cells and immature neurons. Various conditions can easily induce dematuration, such as inflammation and hyper-excitation of neurons, and therefore, the markers for neural progenitor cells and immature neurons may not necessarily serve as markers for AHN. Thus, the aforementioned studies have not presented definitive evidence for the persistence of hippocampal neurogenesis throughout adult life in humans, and we would like to emphasize that those markers should be used cautiously when presented as evidence for AHN. Increasing AHN has been considered as a therapeutic target for Alzheimer’s disease (AD); however, given that immature neuronal markers can be re-expressed in mature adult neurons, independent of AHN, in various disease conditions including AD, strategies to increase the expression of these markers in the DG may be ineffective or may worsen the symptoms of such diseases

Expression of tryptophan 2,3-dioxygenase in mature granule cells of the adult mouse dentate gyrus

New granule cells are continuously generated in the dentate gyrus of the adult hippocampus. During granule cell maturation, the mechanisms that differentiate new cells not only describe the degree of cell differentiation, but also crucially regulate the progression of cell differentiation. Here, we describe a gene, tryptophan 2,3-dioxygenase (TDO), whose expression distinguishes stem cells from more differentiated cells among the granule cells of the adult mouse dentate gyrus. The use of markers for proliferation, neural progenitors, and immature and mature granule cells indicated that TDO was expressed in mature cells and in some immature cells. In mice heterozygous for the alpha-isoform of calcium/calmodulin-dependent protein kinase II, in which dentate gyrus granule cells fail to mature normally, TDO immunoreactivity was substantially downregulated in the dentate gyrus granule cells. Moreover, a 5-bromo-2'-deoxyuridine labeling experiment revealed that new neurons began to express TDO between 2 and 4 wk after the neurons were generated, when the axons and dendrites of the granule cells developed and synaptogenesis occurred. These findings indicate that TDO might be required at a late-stage of granule cell development, such as during axonal and dendritic growth, synaptogenesis and its maturation

Thermal-noise-limited underground interferometer CLIO

We report on the current status of CLIO (Cryogenic Laser Interferometer Observatory), which is a prototype interferometer for LCGT (Large Scale Cryogenic Gravitational-Wave Telescope). LCGT is a Japanese next-generation interferometric gravitational wave detector featuring the use of cryogenic mirrors and a quiet underground site. The main purpose of CLIO is to demonstrate a reduction of the mirror thermal noise by cooling the sapphire mirrors. CLIO is located in an underground site of the Kamioka mine, 1000 m deep from the mountain top, to verify its advantages. After a few years of commissioning work, we have achieved a thermal-noise-limited sensitivity at room temperature. One of the main results of noise hunting was the elimination of thermal noise caused by a conductive coil-holder coupled with a pendulum through magnets.Comment: 10 pages, 6 figures, Proceedings of the 8th Edoardo Amaldi Conference on Gravitational Wave

Electromyographic and Kinematic Trunk Analysis of Boxing during a Dominate Straight Punch

The purpose of this study was to compare the surface electro myogram of trunk muscle activity and the three-dimensional kinematics of the trunk between experienced and novice boxers during straight punch with the rear arm. Fifteen university-age males participated in the study. Participants were ranked as experienced (n=8) or novice (n=7). The straight punch was broken into three phases as Preliminary Movements (PM), Thrown Punch (TP), and Returned Punch (RP). The surface electro myogram captured the activity of the rectus abdominis, external oblique, deltoid, and rectus femoris on the dominant side and the internal oblique-transversus abdominis (IO-TrA) and multifidus on both sides. Three-dimensional motion analysis was performed to calculate the horizontal angle of the Acromial line, the ASIS line and the Greater Trochanter of the femur (GT) line. Results of the surface electro myogram of the IO-TrA on the non-dominant side of the novice group during the PM phase were significantly higher than those of the experienced (p<0.05). Similarly, the IO-TrA of the dominant side of the novice during the TP phase were significantly higher than that of the experienced (p<0.05). In motion analysis, the ASIS line and the GT line were significantly greater in the experienced group compared with the novice (p<0.05). The novice group did not allow the entire trunk to rotate, but rather twisted the thoracolumbar vertebrae to throw the punch. Trunk rotation, not trunk twist, is important to the execution of the straight punch