A microfluidics tool for high-throughput, real-time multimodal imaging of nanoparticle-cell interactions

The increasing use of nanomaterials for biomedical applications has raised the need for efficient, robust and low-cost high-throughput assessment of nanotoxicity and cell-nanoparticle interactions. Microfluidics provides the tools for high-throughput single-cell functional monitoring, while gold nanorods have unique potential for intracellular tracking and can simultaneously be used as drug carriers. Presented here is a miniaturised platform that integrates these features with a multimodal approach to cell imaging. A microfluidic device allows for trapping of an array of singlecells, followed by the controlled delivery of nanoparticles into the cell array and subsequent real-time multimodal imaging of cellular interactions with functionalised nanoparticles. This system has been successfully used to assess cellnanoparticle interactions at the single-cell level

Time-lapse measurement of single-cell response to nanomaterial : a microfluidic approach

This work presents the successful application of a single-cell microfluidic platform for high-throughput, real-time screening of nanoparticle-cell interactions. Taking vaccine delivery as a proof-of-concept application, ovalbumin-conjugated gold nanorods were produced and controllably delivered to primary dendritic cells within the device. Time-lapse imaging enabled monitoring of hundreds of single-cells during exposure to a range of concentrations of nanoparticle conjugates and simultaneous quantification of specific cellular functions. This integrated system provides throughput and statistical data comparable to that obtained with flow cytometry but also offers a novel approach to determine the dynamics of nanoparticle-cell interactions and nanoparticle-mediated antigen delivery with single-cell resolution

Quantum mechanical counterpart of nonlinear optics

Raman-type laser excitation of a trapped atom allows one to realize the quantum mechanical counterpart of phenomena of nonlinear optics, such as Kerr-type nonlinearities, parametric amplification, and multi-mode mixing. Additionally, huge nonlinearities emerge from the interference of the atomic wave function with the laser waves. They lead to a partitioning of the phase space accompanied by a significantly different action of the time evolution in neighboring phase-space zones. For example, a nonlinearly modified coherent "displacement" of the motional quantum state may induce strong amplitude squeezing and quantum interferences.Comment: 6 pages, 4 figures, to be published in Phys. Rev. A 55 (June

Galactic Collapse of Scalar Field Dark Matter

We present a scenario for galaxy formation based on the hypothesis of scalar field dark matter. We interpret galaxy formation through the collapse of a scalar field fluctuation. We find that a cosh potential for the self-interaction of the scalar field provides a reasonable scenario for galactic formation, which is in agreement with cosmological observations and phenomenological studies in galaxies.Comment: 4 pages, 3 figue

Especiação redox de cromo em solo acidentalmente contaminado com solução sulfocrômica.

Determination of Cr(VI) and Cr(III) was studied in soil samples accidentally contaminated with sulphochromic solution. Molecular absorption spectrophotometry based on the diphenylcarbazide method was used for the determination of Cr(VI) after its alkaline extraction. The total chromium concentration was determined using ICP OES. The quantification of Cr(III) was accomplished by subtracting the Cr(VI) concentration from the total chromium concentration. Regardless of the known contamination of the soil samples by sulphochromic solution, concentrations of Cr(VI) were below the detection limit. Addition and recovery experiments for Cr(VI) in soil samples with and without organic matter indicated its influence on the reduction of Cr(VI) to Cr(III)

Determinação de selenometionina por quimiolumiescência em castanha do Brasil.

Selênio é reconhecido como um micronutriente essencial. Porém, a bioutilidade deste elemento está intrísecamente dependente da sua forma química. Espécies orgânicas de selênio são mais facilmente absorvidas por organismos humanos quando comparado a suas espécies inorgânicas. Castanhas do Brasil podem ser consideradas uma boa fonte de selênio, pois apresenta concentrações significativas deste elemento, principalmente na forma de selenometionina.1 Neste trabalho, sistema para análise em fluxo para a determinação de selenometionina em amostras de castanha do Brasil empregando quimioluminescência foi desenvolvido, utilizando uma célula de reação com estrutura em vórtex construída com tecnologia LTCC2

Significance Of Assessment Experiences During Initial Teacher Training In Physical Education

This study investigates how students in the final semester of their teacher training program (licensure) at the Center of Physical Education and Sports (CEFD), Espírito Santo Federal University, Brazil, (re)interpret their assessment experiences, an integral component of their teacher training. It employs the narrative as a theoretical and methodological perspective, and it utilizes student portfolios, as well as focus groups and semi-structured individual interviews as inputs for data generation. Ten students in their eighth, or final, semester participated in this study. These were the total respondents to a "call for volunteers" among the 2014 graduating class. The results suggest that the students believe the assessment processes of their teaching practices in physical education are disjointed. They feel that the disciplines that allow them to review their own performance during teacher training are more efficient and play a stronger role in their education.221627

Metodologias participativas na compreensão do capital social em comunidades rurais no Nordeste paraense.

É essencial o estudo mais aprofundado das varáveis sociais que circundam as comunidades rurais, remetendo uma abordagem multidimensional aos sistemas, cujas contribuições vão além de aspectos meramente tecnológicos ou agronômicos. O objetivo deste trabalho é analisar a utilização de metodologias participativas, como forma de compreender os processos das relações das comunidades parceiras ao projeto Tipitamba com atores institucionais e qual a importância dessas relações para o capital social das comunidades. O estudo baseou-se na combinação de métodos participativos como oficina; diagrama de Veen; conversas com a comunidade sobre como a pesquisa seria realizada; relato das percepções dos agricultores e entrevistas as quais foram norteadas pelo Questionário Integrado para Medir Capital Social ? QI MCS, proposto por Grootaert et. al. (2003), com algumas adaptações a fim de adequá-la aos objetivos da pesquisa e a realidade das comunidades analisadas. As entrevistas semi-estruturadas permitiram analisar dados quantitativos e qualitativos a cerca das dimensões do capital social, dentre os dados quantitativos coletados nas entrevistas está à interação das associações com outros grupos ou instituições, evidenciou-se que existem associações em que o nível de interação com instituições ou grupos com objetivos semelhantes é maior e outras que essa interação é quase inexistente. O Diagrama de Venn se mostrou uma importante ferramenta para que os agricultores pudessem visualizar como estão as relações das comunidades com atores internos e externos, além disso, reconhecerem a importância destes fatores nos processos de decisão e desenvolvimento comunitário