Far-Field Plasmonic Resonance Enhanced Nano-Particle Image Velocimetry within a Micro Channel

In this paper, a novel far-field plasmonic resonance enhanced nanoparticle-seeded Particle Image Velocimetry (nPIV) has been demonstrated to measure the velocity profile in a micro channel. Chemically synthesized silver nanoparticles have been used to seed the flow in the micro channel. By using Discrete Dipole Approximation (DDA), plasmonic resonance enhanced light scattering has been calculated for spherical silver nanoparticles with diameters ranging from 15nm to 200nm. Optimum scattering wavelength is specified for the nanoparticles in two media: water and air. The diffraction-limited plasmonic resonance enhanced images of silver nanoparticles at different diameters have been recorded and analyzed. By using standard PIV techniques, the velocity profile within the micro channel has been determined from the images.Comment: submitted to Review of Scientific Instrument

Improved synthesis of 4-[18F]fluoro-m-hydroxyphenethylguanidine using an iodonium ylide precursor

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/151961/1/jlcr3791.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/151961/2/jlcr3791_am.pd

Extraction of coherent structures in a rotating turbulent flow experiment

The discrete wavelet packet transform (DWPT) and discrete wavelet transform (DWT) are used to extract and study the dynamics of coherent structures in a turbulent rotating fluid. Three-dimensional (3D) turbulence is generated by strong pumping through tubes at the bottom of a rotating tank (48.4 cm high, 39.4 cm diameter). This flow evolves toward two-dimensional (2D) turbulence with increasing height in the tank. Particle Image Velocimetry (PIV) measurements on the quasi-2D flow reveal many long-lived coherent vortices with a wide range of sizes. The vorticity fields exhibit vortex birth, merger, scattering, and destruction. We separate the flow into a low-entropy ``coherent'' and a high-entropy ``incoherent'' component by thresholding the coefficients of the DWPT and DWT of the vorticity fields. Similar thresholdings using the Fourier transform and JPEG compression together with the Okubo-Weiss criterion are also tested for comparison. We find that the DWPT and DWT yield similar results and are much more efficient at representing the total flow than a Fourier-based method. Only about 3% of the large-amplitude coefficients of the DWPT and DWT are necessary to represent the coherent component and preserve the vorticity probability density function, transport properties, and spatial and temporal correlations. The remaining small amplitude coefficients represent the incoherent component, which has near Gaussian vorticity PDF, contains no coherent structures, rapidly loses correlation in time, and does not contribute significantly to the transport properties of the flow. This suggests that one can describe and simulate such turbulent flow using a relatively small number of wavelet or wavelet packet modes.Comment: experimental work aprox 17 pages, 11 figures, accepted to appear in PRE, last few figures appear at the end. clarifications, added references, fixed typo

Genome-Wide Association Study Identifies Loci for Liver Enzyme Concentrations in Mexican Americans: The GUARDIAN Consortium.

ObjectivePopulations of Mexican American ancestry are at an increased risk for nonalcoholic fatty liver disease. The objective of this study was to determine whether loci in known and novel genes were associated with variation in aspartate aminotransferase (AST) (n = 3,644), alanine aminotransferase (ALT) (n = 3,595), and gamma-glutamyl transferase (GGT) (n = 1,577) levels by conducting the first genome-wide association study (GWAS) of liver enzymes, which commonly measure liver function, in individuals of Mexican American ancestry.MethodsLevels of AST, ALT, and GGT were determined by enzymatic colorimetric assays. A multi-cohort GWAS of individuals of Mexican American ancestry was performed. Single-nucleotide polymorphisms (SNP) were tested for association with liver outcomes by multivariable linear regression using an additive genetic model. Association analyses were conducted separately in each cohort, followed by a nonparametric meta-analysis.ResultsIn the PNPLA3 gene, rs4823173 (P = 3.44 × 10-10 ), rs2896019 (P = 7.29 × 10-9 ), and rs2281135 (P = 8.73 × 10-9 ) were significantly associated with AST levels. Although not genome-wide significant, these same SNPs were the top hits for ALT (P = 7.12 × 10-8 , P = 1.98 × 10-7 , and P = 1.81 × 10-7 , respectively). The strong correlation (r2  = 1.0) for these SNPs indicated a single hit in the PNPLA3 gene. No genome-wide significant associations were found for GGT.ConclusionsPNPLA3, a locus previously identified with ALT, AST, and nonalcoholic fatty liver disease in European and Japanese GWAS, is also associated with liver enzymes in populations of Mexican American ancestry

Robust cell tracking in epithelial tissues through identification of maximum common subgraphs

Tracking of cells in live-imaging microscopy videos of epithelial sheets is a powerful tool for investigating fundamental processes in embryonic development. Characterizing cell growth, proliferation, intercalation and apoptosis in epithelia helps us to understand how morphogenetic processes such as tissue invagination and extension are locally regulated and controlled. Accurate cell tracking requires correctly resolving cells entering or leaving the field of view between frames, cell neighbour exchanges, cell removals and cell divisions. However, current tracking methods for epithelial sheets are not robust to large morphogenetic deformations and require significant manual interventions. Here, we present a novel algorithm for epithelial cell tracking, exploiting the graph-theoretic concept of a ‘maximum common subgraph’ to track cells between frames of a video. Our algorithm does not require the adjustment of tissue-specific parameters, and scales in sub-quadratic time with tissue size. It does not rely on precise positional information, permitting large cell movements between frames and enabling tracking in datasets acquired at low temporal resolution due to experimental constraints such as phototoxicity. To demonstrate the method, we perform tracking on the Drosophila embryonic epidermis and compare cell–cell rearrangements to previous studies in other tissues. Our implementation is open source and generally applicable to epithelial tissues

Tangling clustering of inertial particles in stably stratified turbulence

We have predicted theoretically and detected in laboratory experiments a new type of particle clustering (tangling clustering of inertial particles) in a stably stratified turbulence with imposed mean vertical temperature gradient. In this stratified turbulence a spatial distribution of the mean particle number density is nonuniform due to the phenomenon of turbulent thermal diffusion, that results in formation of a gradient of the mean particle number density, \nabla N, and generation of fluctuations of the particle number density by tangling of the gradient, \nabla N, by velocity fluctuations. The mean temperature gradient, \nabla T, produces the temperature fluctuations by tangling of the gradient, \nabla T, by velocity fluctuations. These fluctuations increase the rate of formation of the particle clusters in small scales. In the laboratory stratified turbulence this tangling clustering is much more effective than a pure inertial clustering that has been observed in isothermal turbulence. In particular, in our experiments in oscillating grid isothermal turbulence in air without imposed mean temperature gradient, the inertial clustering is very weak for solid particles with the diameter 10 microns and Reynolds numbers Re =250. Our theoretical predictions are in a good agreement with the obtained experimental results.Comment: 16 pages, 4 figures, REVTEX4, revised versio

Little Earth Experiment: An instrument to model planetary cores

In this paper, we present a new experimental facility, Little Earth Experiment, designed to study the hydrodynamics of liquid planetary cores. The main novelty of this apparatus is that a transparent electrically conducting electrolyte is subject to extremely high magnetic fields (up to 10 T) to produce electromagnetic effects comparable to those produced by moderate magnetic fields in planetary cores. This technique makes it possible to visualise for the first time the coupling between the principal forces in a convection-driven dynamo by means of Particle Image Velocimetry (PIV) in a geometry relevant to planets. We first present the technology that enables us to generate these forces and implement PIV in a high magnetic field environment. We then show that the magnetic field drastically changes the structure of convective plumes in a configuration relevant to the tangent cylinder region of the Earth's core. Published by AIP Publishing

An assessment on the unsteady flow distortion generated by an S-duct intake

Closer integration between the fuselage and the propulsion system is expected for futureaircraft toreducefuel consumption, emissions, weight and drag. The use of embedded or partially embedded propulsion systems may require the use of complex intakes. However, thiscanresult in unsteady flow distortion which can adversely affect the propulsion system efficiency and stability. This works assesses the characteristics of the unsteady flow with a view to the potential flow distortion presented to the compression system.Particle image velocimetry is used to measure the flow distortion generated by an S-shaped intake.The time-resolved tracking of the idealized relative incidence angle revealed that most frequent distortion events exhibited90°exposure sector and upto±5°meanrelativeincidence. The imposition of a thicker boundary at the S-duct inlet increased the probability of distortion events that are characterized by a longer exposure sector and higher relative incidence angles.Because of these characteristics, thedistortion caused by the S-duct intake could induce instabilities that are detrimental for the propulsion system performances and stability. Overall, this work proposes a new method to assess thepossible relativeincidence angle on the compressor rotor taking into account the intake flow unsteadiness