Progesterone induces surface expression of EMP2 in mouse endometrium in vivo

Mice were injected with progesterone or sesame oil (control) daily for up to 5 days. Tissue was harvested at days 1, 2, and 4 of treatment or 3 days following completion of treatment, processed for immunohistochemistry, and then stained for EMP2 expression (A-D) or a vehicle control (E). (A) 24 hours of progesterone treatment; (B) 48 hours of treatment; (C) 4 days of treatment; (D) 3 days following treatment; (E) representative control treatment at 4 days. Magnification: (left) 100×; (right) 400×. The experiment was repeated at least 3 times with similar results; representative images are shown.<p><b>Copyright information:</b></p><p>Taken from "Steroid hormone regulation of EMP2 expression and localization in the endometrium"</p><p>Reproductive biology and endocrinology : RB&E 2008;6():15-15.</p><p>Published online 9 Apr 2008</p><p>PMCID:PMC2329639.</p><p></p

(A) Normal human endometrium specimens from subjects in proliferative and secretory phases were immunoblotted using EMP2 antisera

β-actin was used as a loading control. (B) Statistical analysis of EMP2 expression levels in proliferative (n = 3: subphase unspecified) and secretory (n = 3: one post-ovulatory day 3, one late, one unspecified) endometrial specimens from six independent patients. Data were analyzed using a 2-tailed, unpaired student t-test with a 95% confidence interval. *Changes in expression between the proliferative and secretory endometrium were significant, p = 0.038.<p><b>Copyright information:</b></p><p>Taken from "Steroid hormone regulation of EMP2 expression and localization in the endometrium"</p><p>http://www.rbej.com/content/6/1/15</p><p>Reproductive biology and endocrinology : RB&E 2008;6():15-15.</p><p>Published online 9 Apr 2008</p><p>PMCID:PMC2329639.</p><p></p