Allelic effects on starch structure and properties of six starch biosynthetic genes in a rice recombinant inbred line population

BACKGROUND: The genetic diversity of six starch biosynthetic genes (Wx, SSI, SSIIa, SBEI, SBEIIa and SBEIIb) in indica and japonica rices opens an opportunity to produce a new variety with more favourable grain starch quality. However, there is limited information about the effects of these six gene allele combinations on starch structure and properties. A recombinant inbred line population from a cross between indica and japonica varieties offers opportunities to combine specific alleles of the six genes. RESULTS: The allelic (indica vs japonica) effects of six starch biosynthetic genes on starch structure, functional properties, and abundance of granule bound proteins in rice grains were investigated in a common genetic background using a recombinant inbred line population. The indica Wx (Wxi) allele played a major role while indica SSI (SSIi), japonica SSIIa (SSIIaj) and indica SBEI (SBEIi) alleles had minor roles on the increase of amylose content. SSIIaj and japonica SBEIIb (SBEIIbj) alleles had a major and a minor role on high ratio of ∑DP ≤ 10 to ∑DP ≤ 24 fractions (RCL10/24), respectively. Both major alleles (Wxi and SSIIaj) reduced peak viscosity (PV), onset, peak and end gelatinization temperatures (GTs) of amylopectin, and increased amylose-lipid complex dissociation enthalpy compared with their counterpart-alleles, respectively. SBEIIai and SBEIIbj decreased PV, whereas SSIi and SBEIIbj decreased FV. SBEIi reduced setback viscosity and gelatinization enthalpy. RCL10/24 of chain length distribution in amylopectin is negatively correlated with PV and BD of paste property and GTs of thermal properties. We also report RILs with superior starch properties combining Wxi, SSIj, SSIIaj, SBEIi and SBEIIbj alleles. Additionally, a clear relation is drawn to starch biosynthetic gene alleles, starch structure, properties, and abundance of granule bound starch biosynthetic enzymes inside starch granules. CONCLUSIONS: Rice Wxi and SSIIaj alleles play major roles, while SSIi, SBEIi, SBEIIai and SBEIIbj alleles have minor roles in the determination of starch properties between indica and japonica rice through starch structural modification. The combination of these alleles is a key factor for starch quality improvement in rice breeding programs. RCL10/24 value is critical for starch structure and property determination.Jixun Luo was supported by CSC (Chinese Scholarship Council) and Australian National University scholarships. This work was funded by CSIRO Food Future National Research Flagship

A New Approach to Random Access: Reliable Communication and Reliable Collision Detection

This paper applies Information Theoretic analysis to packet-based random multiple access communication systems. A new channel coding approach is proposed for coding within each data packet with built-in support for bursty traffic properties, such as message underflow, and for random access properties, such as packet collision detection. The coding approach does not require joint communication rate determination either among the transmitters or between the transmitters and the receiver. Its performance limitation is characterized by an achievable region defined in terms of communication rates, such that reliable packet recovery is supported for all rates inside the region and reliable collision detection is supported for all rates outside the region. For random access communication over a discrete-time memoryless channel, it is shown that the achievable rate region of the introduced coding approach equals the Shannon information rate region without a convex hull operation. Further connections between the achievable rate region and the Shannon information rate region are developed and explained.Comment: submitted to IEEE Transactions on Information Theor

Reagentless glucose biosensor based on the direct electrochemistry of glucose oxidase on carbon nanotube-modified electrodes

The direct electrochemistry of glucose oxidase (GOD) was revealed at a carbon nanotube (CNT)-modified glassy carbon electrode, where the enzyme was immobilized with a chitosan film containing gold nanoparticles. The immobilized GOD displays a pair of redox peaks in pH 7.4 phosphate buffer solutions (PBS) with the formal potential of about -455 mV (vs. Ag/AgCl) and shows a surface-controlled electrode process. Bioactivity remains good, along with effective catalysis of the reduction of oxygen. In the presence of dissolved oxygen, the reduction peak current decreased gradually with the addition of glucose, which could be used for reagentless detection of glucose with a linear range from 0.04 to 1.0 mM. The proposed glucose biosensor exhibited high sensitivity, good stability and reproducibility, and was also insensitive to common interferences such as ascorbic and uric acid. The excellent performance of the reagentless biosensor is attributed to the effective enhancement of electron transfer between enzyme and electrode surface by CNTs, and the biocompatible environment that the chitosan film containing gold nanoparticles provides for immobilized GOD

Dynamics of Eddy-Driven Low-Frequency Dipole Modes. Part I: A Simple Model of North Atlantic Oscillations

A simple theoretical model is proposed to clarify how synoptic-scale waves drive the life cycle of the North Atlantic Oscillation (NAO) with a period of nearly two weeks. This model is able to elucidate what determines the phase of the NAO and an analytical solution is presented to indicate a high similarity between the dynamical processes of the NAO and zonal index, which is not derived analytically in previous theoretical studies. It is suggested theoretically that the NAO is indeed a nonlinear initial-value problem, which is forced by both preexisting planetary-scale and synoptic-scale waves. The eddy forcing arising from the preexisting synoptic-scale waves is shown to be crucial for the growth and decay of the NAO, but the preexisting low-over-high (high-over-low) dipole planetary-scale wave must be required to match the preexisting positive-over-negative (negative-over-positive) dipole eddy forcing so as to excite a positive (negative) phase NAO event. The positive and negative feedbacks of the preexisting dipole eddy forcing depending upon the background westerly wind seem to dominate the life cycle of the NAO and its life period. An important finding in the theoretical model is that negative-phase NAO events could be excited repeatedly after the first event has decayed, but for the positive phase downstream isolated dipole blocks could be produced after the first event has decayed. This is supported by observed cases of the NAO events presented in this paper. In addition, a statistical study of the relationship between the phase of the NAO and blocking activity over Europe in terms of the seasonal mean NAO index shows that blocking events over Europe are more frequent and long-lived for strong positive-phase NAO years, indicating that the positivephase NAO favors the occurrence of European blocking events

Dynamics of Eddy-Driven Low-Frequency Dipole Modes. Part II: Free Mode Characteristics of NAO and Diagnostic Study

Through calculating the scatter diagrams of the streamfunction ( P or T) versus potential vorticity (PV)(qP or qT), where P and T are the planetary-scale streamfunction and total streamfunction, respectively, and using a weakly nonlinear NAO model proposed in Part I of this paper, it is suggested that negative- and positive-phase NAO events may approximately correspond to free modes even though driven by synopticscale eddies. In a planetary-scale field, the qP( P) scatter diagram of an NAO event exhibits a linear multivalued functional relationship in a narrow region for the negative phase, but exhibits a linear singlevalued functional relationship during the positive phase. It was also found that there is no steepening of the slope of the main straight line in the qP( P) scatter diagrams for two phases of the NAO event. Instead, the slope of the straight line in the scatterplots is time independent throughout the life cycle of the NAO event. However, when synoptic-scale eddies are included in the streamfunction field, the qT( T) scatter diagram of the negative-phase NAO event shows a trend toward steepening during the intensification phase, and this tendency reverses during the decay phase. During the positive NAO phase the slope of the qt( T) scatter diagram shoals during the intensification phase and then steepens during the decay phase. Thus, it appears that the steepening and shoaling of the scatter diagrams of the streamfunction versus PV for the negative and positive-phase NAO events are attributed to the effect of synoptic-scale eddies that force NAO events to form. Diagnostic studies using both composite and unfiltered fields of observed NAO events are presented to confirm these conclusions

Enhancement of a conducting polymer-based biosensor using carbon nanotube-doped polyaniline.

A biosensor with improved performance was developed through the immobilization of horseradish peroxidase (HRP) onto electropolymerized polyaniline (PANI) films doped with carbon nanotubes (CNTs). The effects of electropolymerization cycle and CNT concentration on the response of the biosensor toward H2O2 were investigated. It was found that the integration of CNTs into the biosensor system could increase the amount and stability of immobilized enzyme, and greatly enhance the biosensor response. Compared with the biosensor fabricated without CNTs, the proposed biosensor exhibited enhanced stability and approximately eight-fold higher sensitivity. A linear range from 0.2 to 19μM for the detection of H2O2 was observed, with a detection limit of 68 nM at a signal-to-noise ratio of 3 and a response time of less than 5s

Observational tests of hurricane intensity estimations using GPS radio occultations

This study presents a novel approach to estimating the intensity of hurricanes using temperature profiles from Global Positioning System radio occultation (GPSRO) measurements. Previous research has shown that the temperature difference between the ocean surface and the eyewall outflow region defines hurricanes' thermodynamic efficiency, which is directly proportional to the storm's intensity. Outflow temperatures in the eyewall region of 27 hurricanes in 2004–2011 were obtained from GPSRO observations. These observations, along with ocean surface temperatures from NASA Modern Era-Retrospective Analysis for Research and Applications, made it possible to estimate hurricane intensities using a simplified hurricane model. Our preliminary results are quantitatively consistent with best-track values from the National Hurricane Center within 9.4%. As a by-product of our study, we present for the first time GPSRO vertical temperature profiles in the vicinity of the eyewall region of hurricanes, which we compared with collocated temperature profiles from the European Centre for Medium-Range Weather Forecasts Reanalysis Interim (ERA-Interim). Some of the GPSRO data sets reveal a double tropopause in the vicinity of the eyewall—a characteristic that we do not see in ERA-Interim. We conclude that GPSRO observations can be of supplementary assistance in augmenting existing data sets used in hurricane intensity estimation. GPSROs' cloud-penetrating capability and high vertical resolution can be useful in providing soundings in the area close to the eyewall region of hurricanes revealing detailed information about their thermal structure, potentially advancing our current knowledge of their dynamics, evolution, and physics