Electrochemical sensing of doxorubicin hydrochloride under sodium alginate antifouling conditions using silver nanoparticles modified glassy carbon electrodes

Doxorubicin (DOX) is a highly effective anticancer drug with a narrow therapeutic window; thus, sensitive and timely detection of DOX is crucial. Using electrodeposition of silver nanoparticles (AgNPs) and electropolymerization alginate (Alg) layers on the surface of a glassy carbon electrode, a novel electrochemical probe was constructed (GCE). The fabricated AgNPs/poly-Alg-modified GCE probe was utilized for the quantification of DOX in unprocessed human plasma samples. For the electrodeposition of AgNPs and electropolymerization of alginate (Alg) layers on the surface of GCE, cyclic voltammetry (CV) was used in the potential ranges of −2.0 to 2.0 V and −0.6 to 0.2 V, respectively. The electrochemical activity of DOX exhibited two oxidation processes at the optimum pH value of 5.5 on the surface of the modified GCE. The DPV spectra of poly(Alg)/AgNPs modified GCE probe toward consecutive concentrations of DOX in plasma samples demonstrated wide dynamic ranges of 15 ng/mL-0.1 μg/mL and 0.1–5.0 μg/mL, with a low limit of quantification (LLOQ) of 15 ng/mL. The validation results indicated that the fabricated electrochemical probe might serve as a highly sensitive and selective assay for the quantification of DOX in patient samples. As an outstanding feature, the developed probe could detect DOX in unprocessed plasma samples and cell lysates without the requirement for pretreatment

An ultrasensitive and preprocessing-free electrochemical platform for the detection of doxorubicin based on tryptophan/polyethylene glycol-cobalt ferrite nanoparticles modified electrodes

© 2022 Elsevier B.V.Doxorubicin (DOX) is an anticancer drug which can effectively inhibit the growth of cancer cells and aids the immune-mediated elimination of tumoral cells. Developing a new technique for analysis of DOX in clinical fluids is highly required. Here, a novel electrochemical sensor was designed using tryptophan (Trp)/(polyethylene glycol)PEGylated-CoFe2O4 nanoparticles to modify glassy carbon electrodes’ surface and was utilized to determine DOX in unprocessed human plasma samples. PEGylated-CoFe2O4 nanoparticles were coated on the surface of the glassy carbon electrode to provide the PEGylated-CoFe2O4/GCE probe. Under optimized conditions, the low limit of quantification (LLOQ) of the proposed sensor was 30 ng/mL and the linear ranges for the determination of DOX were 30 ng/mL to 1.0 μg/mL and 1.0 μg/mL to 5.0 μg/mL, respectively. PEG molecules provided an antifouling effect to prevent precipitation of the macromolecules on the surface of the fabricated electrode. Obtained results indicated that the suggested electrochemical sensor can be utilized for specific and sensitive determination of DOX in plasma samples