2 research outputs found
Modulation of a Photoswitchable Dual-Color Quantum Dot containing a Photochromic FRET Acceptor and an Internal Standard
Photoswitchable semiconductor nanoparticles, quantum
dots (QDs),
couple the advantages of conventional QDs with the ability to reversibly
modulate the QD emission, thereby improving signal detection by rejection
of background signals. Using a simple coating methodology with polymers
incorporating a diheteroarylethene photochromic FRET acceptor as well
as a spectrally distinct organic fluorophore, photoswitchable QDs
were prepared that are small, biocompatible, and feature ratiometric
dual emission. With programmed irradiation, the fluorescence intensity
ratio can be modified by up to ∼100%
Photocontrolled Binding and Binding-Controlled Photochromism within Anthracene-Modified DNA
Modified DNA strands undergo a reversible light-induced
reaction
involving the intramolecular photodimerization of two appended anthracene
tags. The photodimers exhibit markedly different binding behavior
toward a complementary strand that depends on the number of bases
between the modified positions. By preforming the duplex, photochromism
can be suppressed, illustrating dual-mode gated behavior