Allergic bronchopulmonary aspergillosis: diagnostic and treatment challenges

Allergic bronchopulmonary aspergillosis (ABPA) is a pulmonary disorder, occurring mostly in asthmatic and cystic fibrosis patients, caused by an abnormal T-helper 2 lymphocyte response of the host to Aspergillus fumigatus antigens. ABPA diagnosis is defined by clinical, laboratory and radiological criteria including active asthma, immediate skin reactivity to A. fumigatus antigens, total serum IgE levels>1000 IU/mL, fleeting pulmonary parenchymal opacities and central bronchiectases that represent an irreversible complication of ABPA. Despite advances in our understanding of the role of the allergic response in the pathophysiology of ABPA, pathogenesis of the disease is still not completely clear. In addition, the absence of consensus regarding its prevalence, diagnostic criteria and staging limits the possibility of diagnosing the disease at early stages. This may delay the administration of a therapy that can potentially prevent permanent lung damage. Long-term management is still poorly studied. Present primary therapies, based on clinical experience, are not yet standardized. These consist in oral corticosteroids, which control acute symptoms by mitigating the allergic inflammatory response, azoles and, more recently, anti-IgE antibodies. The latter two are used as a steroid-sparing agent to prolong the remission stage of the disease. Anti-IgE antibodies also have immunomodulatory properties. At present, the only way to bypass these limits and allow for an early diagnosis, is to assume ABPA in all patients with difficult-to-control asthma or cystic fibrosis. They should then be screened for sensitization to A. fumigatus antigens and, if positive, monitored more closely. Future controlled studies are needed to standardize present therapy, standardize cut-off values of various investigations, define the role of different novel immunomodulatory therapies, define the role of novel assays (such as recombinant A. fumigatus antigens and CCL17) and confirm new diagnostic and staging criteria

A Novel MAC Protocol for Low Datarate Cooperative Mobile Robot Teams

Cooperative mobile robot applications enable robots to perform tasks that are more complex than those that each single robot can perform alone. In this application context, communication networks play a very important role, as they have to cope with strict requirements (e.g., in terms of mobility, reliability, and bounded latencies). Recent cooperative robot applications foresee the support of low datarate communication technologies, that provide, among other benefits, lower energy consumption and easy integration with Wireless Sensor Networks (WSNs). Unfortunately, the state-of-the-art solutions either entail high costs and complexity or are not suitable for low data rate communications. Consequently, novel solutions for cooperating robots are required. For this reason, this paper presents RoboMAC, a new MAC protocol for mobile cooperating robots that enables the integration of robots with WSNs, supports mobility and real-time communications, and provides high scalability. The paper also presents a proof-of-concept implementation that proves the feasibility of the RoboMAC protocol on COTS devices

Priority-Based Bandwidth Management in Virtualized Software-Defined Networks

In Industrial Internet of Things (IoT) applications, when the network size increases and different types of flows share the bandwidth, the demand for flexible and efficient management of the communication network is compelling. In these scenarios, under varying workload and flow priorities, the combined use of Software-Defined Networking (SDN) and Network Virtualization (NV) is a promising solution, as such techniques allow to reduce the network management complexity. This work presents the PrioSDN Resource Manager (PrioSDN_RM), a resource management mechanism based on admission control for virtualized SDN-based networks. The proposed combination imposes bounds on the resource utilization for the virtual slices, which therefore share the network links, while maintaining isolation from each other. The presented approach exploits a priority-based runtime bandwidth distribution mechanism to dynamically react to load changes (e.g., due to alarms). The paper describes the design of the approach and provides experimental results obtained on a real testbed

Immunohistochemical Expression of Matrix Metalloprotease-2 and Matrix Metalloprotease-9 in the Disks of Patients with Temporomandibular Joint Dysfunction

Purpose Matrix metalloproteases (MMPs) are tissue-remodeling enzymes that function during the remodeling process, such as in immune-inflammatory diseases. Metalloprotease-2 (MMP-2) and metalloprotease-9 (MMP-9) are gelatinases that degrade several types of extracellular matrix collagen. It is hypothesized that in temporomandibular joint (TMJ) dysfunction, MMP-2 and MMP-9 expression levels may be elevated. Therefore, the objective of this study is to determine the association of MMP-2 and MMP-9 expression with temporomandibular joint dysfunction using an immunohistochemical approach to evaluate the joint disk. Material and Methods A total of 45 human temporomandibular joint samples were collected, with 36 samples in the test group (patients with anterior disk displacement with reduction (n = 29) and without reduction (n = 7)) and nine samples in the control group. The immunostaining of the TMJ disks was statistically compared between the groups (P \u3c 0.05). Results There was a statistically significant difference for the area of MMP-2 immunostaining between the control group and the displacement disks with reduction group (ADDwR) (P = 0.048) and between the groups with disk displacement and without reduction (ADDwoR) (P = 0.029). The expression of MMP-2 was significantly elevated in the ADDwoR group. Conclusion No statistically significant difference was found between the variable area of MMP-9 expression in the disk with and without disk displacement, as determined by immunohistochemical analysis. However, there was an elevation of MMP-2 expression in the disks of patients with displacement and without reduction (more severe alteration)

Single cell oils of the cold-adapted oleaginous yeast Rhodotorula glacialis DBVPG 4785

<p>Abstract</p> <p>Background</p> <p>The production of microbial lipids has attracted considerable interest during the past decade since they can be successfully used to produce biodiesel by catalyzed transesterification with short chain alcohols. Certain yeast species, including several psychrophilic isolates, are oleaginous and accumulate lipids from 20 to 70% of biomass under appropriate cultivation conditions. Among them, <it>Rhodotorula glacialis </it>is a psychrophilic basidiomycetous species capable to accumulate intracellular lipids.</p> <p>Results</p> <p><it>Rhodotorula glacialis </it>DBVPG 4785 is an oleaginous psychrophilic yeast isolated from a glacial environment. Despite its origin, the strain abundantly grew and accumulated lipids between -3 to 20°C. The temperature did not influence the yield coefficients of both biomass and lipids production, but had positive effect on the growth rate and thus on volumetric productivity of lipid. In glucose-based media, cellular multiplication occurred first, while the lipogenic phase followed whenever the culture was limited by a nutrient other than glucose. The extent of the carbon excess had positive effects on triacylglycerols production, that was maximum with 120 g L^-1glucose, in terms of lipid concentration (19 g L^-1), lipid/biomass (68%) and lipid/glucose yields (16%). Both glucose concentration and growth temperature influenced the composition of fatty acids, whose unsaturation degree decreased when the temperature or glucose excess increased.</p> <p>Conclusions</p> <p>This study is the first proposed biotechnological application for <it>Rhodotorula glacialis </it>species, whose oleaginous biomass accumulates high amounts of lipids within a wide range of temperatures through appropriate cultivation C:N ratio. Although <it>R. glacialis </it>DBVPG 4785 is a cold adapted yeast, lipid production occurs over a broad range of temperatures and it can be considered an interesting microorganism for the production of single cell oils.</p

Multi-Hop Real-Time Communications Over Bluetooth Low Energy Industrial Wireless Mesh Networks

Industrial wireless sensor networks (IWSNs) are used to acquire sensor data that need real-time processing, therefore they require predictable behavior and real-time guarantees. To be cost effective, IWSNs are also expected to be low cost and low power. In this context, Bluetooth low energy (BLE) is a promising technology, as it allows implementing low-cost industrial networks. As BLE is a short-range technology, a multihop mesh network is needed to cover a large area. Nevertheless, the recently published Bluetooth mesh networking specifications do not provide support for real-time communications over multihop mesh networks. To overcome this limitation, this paper proposes the multihop real-time BLE (MRT-BLE) protocol, a real-time protocol developed on top of BLE, that allows for bounded packet delays over mesh networks. MRT-BLE also provides priority support. This paper describes in detail the MRT-BLE protocol and how to implement it on commercial-off-the-shelf devices. Two kinds of performance evaluation for the MRT-BLE protocol are provided. The first one is a worst case end-to-end delay analysis, while the second one is based on the experimental results obtained through measurements on a real testbed

Successful treatment with omalizumab of allergic bronchopulmonary aspergillosis in patients with cystic fibrosis: Case reports and literature review.

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Up-To-Date Review About Minipuberty and Overview on Hypothalamic-Pituitary-Gonadal Axis Activation in Fetal and Neonatal Life

Minipuberty consists of activation of the hypothalamic-pituitary-gonadal (HPG) axis during the neonatal period, resulting in high gonadotropin and sex steroid levels, and occurs mainly in the first 3–6 months of life in both sexes. The rise in the levels of these hormones allows for the maturation of the sexual organs. In boys, the peak testosterone level is associated with penile and testicular growth and the proliferation of gonadic cells. In girls, the oestradiol levels stimulate breast tissue, but exhibit considerable fluctuations that probably reflect the cycles of maturation and atrophy of the ovarian follicles. Minipuberty allows for the development of the genital organs and creates the basis for future fertility, but further studies are necessary to understand its exact role, especially in girls. Nevertheless, no scientific study has yet elucidated how the HPG axis turns itself off and remains dormant until puberty. Additional future studies may identify clinical implications of minipuberty in selected cohorts of patients, such as premature and small for gestational age infants. Finally, minipuberty provides a fundamental 6-month window of the possibility of making early diagnoses in patients with suspected sexual reproductive disorders to enable the prompt initiation of treatment rather than delaying treatment until pubertal failure

Immunohistochemical Analysis of IL-1 beta in the Discs of Patients with Temporomandibular Joint Dysfunction

Purpose: Interleukin-1 beta (IL-1β) is a cytokine that participates in the regulation of immune responses and inflammatory reactions. It is hypothesized that IL-1 levels may be elevated in patients suffering from temporomandibular joint dysfunction. The purpose of this study was to determine the association of IL-1β expression with TMD using an immunohistochemical approach to evaluate the joint disc. Materials and methods: A total of 39 human temporomandibular joint disc samples were collected, with 31 samples in the test group. Nineteen of the test group samples were from discs of patients with anterior disc displacement with reduction, and 12 of the samples were from patients with anterior disc displacement without reduction. Eight control samples were used in the control group. The samples were immunostained and evaluated on both quantity and intensity of staining. Results: There was a statistically significant difference (p \u3c 0.05) between the control and test groups for both quantity and intensity of staining. Conclusion: IL-1β plays a role in the inflammatory process and degradation of TMJ discs in patients with TMJ dysfunctions

Hashimoto's Disease and Thyroid Cancer in Children: Are They Associated?

Hashimoto's thyroiditis (HT) is the most common cause of thyroid disease in children and adolescents. Along with significant modifications of thyroid function, HT in pediatric age can be accompanied by relevant thyroid structural alterations. Over time, benign thyroid nodules, carcinoma and, rarely, primary non-Hodgkin lymphoma can develop. However, the relationships between HT and neoplasms are poorly defined. The main aim of this paper is to discuss what is presently known regarding the coexistence of HT and thyroid tumors. Moreover, we attempt to define the pathogenesis of cancer development in children with HT. Literature analysis showed that despite its rarity and relatively promising prognosis, thyroid cancer is associated with HT. Although not all reasons for the coexistence of these diseases are clearly defined, children with HT should be considered at higher risk for thyroid cancer development. Strict correlations between high levels of serum TSH and anti-thyroid antibodies with cancer must be remembered. The same is true for the presence of nodules, especially if multiple nodules are present and ultrasonography and thyroid fine needle aspiration cytology should be promptly used in uncertain cases