Selection and stabilization of endocytic sites by Ede1, a yeast functional homologue of human Eps15.

During clathrin-mediated endocytosis (CME), endocytic-site maturation can be divided into two stages corresponding to the arrival of the early and late proteins at the plasma membrane. The early proteins are required to capture cargo and position the late machinery, which includes proteins involved in actin assembly and membrane scission. However, the mechanism by which early-arriving proteins select and stabilize endocytic sites is not known. Ede1, one of the earliest proteins recruited to endocytic sites, facilitates site initiation and stabilization. Deletion of EDE1 results in fewer CME initiations and defects in the timing of vesicle maturation. Here we made truncation mutants of Ede1 to better understand how different domains contribute to its recruitment to CME sites, site selection, and site maturation. We found that the minimal domains required for efficient Ede1 localization at CME sites are the third EH domain, the proline-rich region, and the coiled-coil region. We also found that many strains expressing ede1 truncations could support a normal rate of site initiation but still had defects in site-maturation timing, indicating separation of Ede1 functions. When expressed in yeast, human Eps15 localized to the plasma membrane, where it recruited late-phase CME proteins and supported productive endocytosis, identifying it as an Ede1 functional homologue

Gamma-Ray Burst Afterglows: Effects of Radiative Corrections and Nonuniformity of the Surrounding Medium

The afterglow of a gamma-ray burst (GRB) is commonly thought to be due to continuous deceleration of a relativistically expanding fireball in the surrounding medium. Assuming that the expansion of the fireball is adiabatic and that the density of the medium is a power-law function of shock radius, viz., $n_{ext}\propto R^{-k}$, we analytically study the effects of the first-order radiative correction and the nonuniformity of the medium on a GRB afterglow. We first derive a new relation among the observed time, the shock radius and the fireball's Lorentz factor: $t_\oplus=R/4(4-k)\gamma^2c$, and also derive a new relation among the comoving time, the shock radius and the fireball's Lorentz factor: $t_{co}=2R/(5-k)\gamma c$. We next study the evolution of the fireball by using the analytic solution of Blandford and McKee (1976). The radiation losses may not significantly influence this evolution. We further derive new scaling laws both between the X-ray flux and observed time and between the optical flux and observed time. We use these scaling laws to discuss the afterglows of GRB 970228 and GRB 970616, and find that if the spectral index of the electron distribution is $p=2.5$, implied from the spectra of GRBs, the X-ray afterglow of GRB970616 is well fitted by assuming $k=2$.Comment: 17 pages, no figures, Latex file, MNRAS in pres

Spin dependent transport in ferromagnetic/superconductor/ferromagnetic single electron transistor

Ferromagnetic single electron transistors with Al islands and orthogonal ferromagnetic leads (Co) are fabricated using ebeam lithography followed by shadow evaporation techniques. I-V characteristics exhibit typical single electron tunneling effects. Transport measurements performed in external magnetic field show that, when the two ferromagnetic leads are in antiparallel configuration, spin imbalance leads to a suppression of superconductivity.Comment: 3 pages, 4 figure

From Electrons to Finite Elements: A Concurrent Multiscale Approach for Metals

We present a multiscale modeling approach that concurrently couples quantum mechanical, classical atomistic and continuum mechanics simulations in a unified fashion for metals. This approach is particular useful for systems where chemical interactions in a small region can affect the macroscopic properties of a material. We discuss how the coupling across different scales can be accomplished efficiently, and we apply the method to multiscale simulations of an edge dislocation in aluminum in the absence and presence of H impurities.Comment: 4 page