Anticoagulant activity of a natural protein purified from <i>Hypomesus olidus</i>

<p>A novel anticoagulant protein (E-II-1) was separated and purified from <i>Hypomesus olidus</i>, a unique freshwater fish in northern China. E-II-1 had a molecular mass of approximately 40 kDa with no subunits. The high content of hydrophobic amino acids and negatively charged amino acids in E-II-1 demonstrated that the amino acid compositions might contribute to the anticoagulant activity. E-II-1 contained <i>α</i>-helices 16.75%, <i>β</i>-sheets 42.67%, <i>β</i>-turn 25.58% and random coil 15.00%. <i>In vitro</i> blood coagulation time assay, E-II-1 significantly prolonged the activated partial thrombin time in a dose-dependent manner. Results indicated that E-II-1 acted as anticoagulants through the endogenous pathway with an inhibition of FXa. The specific activity of E-II-1 was 103.50 U/mg at a concentration of 1.00 mg/mL. Therefore, E-II-1 might be one of the promising anticoagulants originated from natural food sources with more safety and less side effects.</p

Two new lignans with antioxidative activities from <i>Jatropha curcas</i>

<div><p>Activity-guided isolation of dried seeds of <i>Jatropha curcas</i> L. led to the isolation of two new lignans along with eight known compounds. These compounds were determined by spectroscopic analysis to be jatrophasin C (<b>1</b>), jatrophasin D (<b>2</b>), β-sitosterol (<b>3</b>), jatrophasin A (<b>4</b>), daucosterol (<b>5</b>), isoamericanol A (<b>6</b>), ( ± )-3,3′-bisdemethylpinoresinol (<b>7</b>), 7′-epi-sesamin-dicatechol (<b>8</b>), isoprincepin (<b>9</b>) and americanol A (<b>10</b>), of which <b>1</b> and <b>2</b> were new compounds. The antioxidative activities along with peroxisome proliferator-activated receptor gamma exciting activity of these compounds were also determined.</p></div

Image_1_Bibliometric analysis study on cognitive function in developmental coordination disorder from 2010 to 2022.PNG

ObjectiveTo identify the research hotspots on cognitive function in developmental coordination disorder (DCD) in recent years, predict the research frontier and development trend, and provide more perspectives for the study of the DCD population.MethodsUsing CiteSpace and VOSviewer software to draw charts, 1,082 pieces of literature about DCD and cognitive function in the Web of Science core collection database from 2010 to 2022 were visually analyzed.Results and conclusionInterest in the cognitive function of DCD has been on the rise in the past 10 years. Over 40 countries and regions, 117 institutions and 200 researchers have participated in the corresponding research, mainly in the United States, and their institutions have published more highly influential results. The hot keywords are DCD, children, attention, working memory, performance, and attention-deficit/hyperactivity disorder (ADHD), and the main research hot topics include functional performance, population, cognitive psychology. The research directions include “DCD,” “Asperger syndrome,” “memory,” “infant,” “clumsiness,” “neurodevelopmental disorder,” “occupational therapy,” “preschool children,” “motor competence,” “model,” and “online control.” Future research should focus on motor imagery and intrinsic models and use more neurophysiological techniques to reveal the cognitive characteristics of children with DCD and develop intervention programs.</p

Table_1_Bibliometric analysis study on cognitive function in developmental coordination disorder from 2010 to 2022.DOCX

ObjectiveTo identify the research hotspots on cognitive function in developmental coordination disorder (DCD) in recent years, predict the research frontier and development trend, and provide more perspectives for the study of the DCD population.MethodsUsing CiteSpace and VOSviewer software to draw charts, 1,082 pieces of literature about DCD and cognitive function in the Web of Science core collection database from 2010 to 2022 were visually analyzed.Results and conclusionInterest in the cognitive function of DCD has been on the rise in the past 10 years. Over 40 countries and regions, 117 institutions and 200 researchers have participated in the corresponding research, mainly in the United States, and their institutions have published more highly influential results. The hot keywords are DCD, children, attention, working memory, performance, and attention-deficit/hyperactivity disorder (ADHD), and the main research hot topics include functional performance, population, cognitive psychology. The research directions include “DCD,” “Asperger syndrome,” “memory,” “infant,” “clumsiness,” “neurodevelopmental disorder,” “occupational therapy,” “preschool children,” “motor competence,” “model,” and “online control.” Future research should focus on motor imagery and intrinsic models and use more neurophysiological techniques to reveal the cognitive characteristics of children with DCD and develop intervention programs.</p

Rapid Decolorization of Phenolic Azo Dyes by Immobilized Laccase with Fe₃O₄/SiO₂ Nanoparticles as Support

Fe₃O₄/SiO₂ nanoparticles with particle size below 30 nm were used as the support for laccase immobilization through glutaraldehyde coupling. Investigation of the immobilized laccase was carried out by X-ray diffractometry (XRD), transmission electron microscopy (TEM), confocal laser scanning microscopy (CLSM), vibrating sample magnetometry (VSM), UV–vis spectrophotometry, and cyclic voltammogram (CV) measurements. Two phenolic azo dyes, Procion Red MX-5B and azophloxine, were selected to investigate the enzyme activity of the immobilized laccase toward degradation of phenolic azo dyes. The immobilized laccase presents unusual performance for dye decolorization and easy separation with an external magnetic field. Finally, the possible mechanism for the unusual decolorization of phenolic azo dyes by the immobilized laccase is discussed

Biosynthetic Baeyer–Villiger Chemistry Enables Access to Two Anthracene Scaffolds from a Single Gene Cluster in Deep-Sea-Derived <i>Streptomyces olivaceus</i> SCSIO T05

Four known compounds, rishirilide B (<b>1</b>), rishirilide C (<b>2</b>), lupinacidin A (<b>3</b>), and galvaquinone B (<b>4</b>), representing two anthracene scaffolds typical of aromatic polyketides, were isolated from a culture of the deep-sea-derived <i>Streptomyces olivaceus</i> SCSIO T05. From the <i>S. olivaceus</i> producer was cloned and sequenced the <i>rsd</i> biosynthetic gene cluster (BGC) that drives rishirilide biosynthesis. The structural gene <i>rsdK</i>₂ inactivation and heterologous expression of the <i>rsd</i> BGC confirmed the single <i>rsd</i> BGC encodes construction of <b>1</b>–<b>4</b> and, thus, accounts for two anthracene scaffolds. Precursor incubation experiments with ¹³C-labeled acetate revealed that a Baeyer–Villiger-type rearrangement plays a central role in construction of <b>1</b>–<b>4</b>. Two luciferase monooxygenase components, along with a reductase component, are presumably involved in the Baeyer–Villiger-type rearrangement reaction enabling access to the two anthracene scaffold variants. Engineering of the <i>rsd</i> BGC unveiled three SARP family transcriptional regulators, enhancing anthracene production. Inactivation of <i>rsdR</i>₄, a MarR family transcriptional regulator, failed to impact production of <b>1</b>–<b>4</b>, although production of <b>3</b> was slightly improved; most importantly <i>rsdR</i>₄ inactivation led to the new adduct <b>6</b> in high titer. Notably, inactivation of <i>rsdH</i>, a putative amidohydrolase, substantially improved the overall titers of <b>1</b>–<b>4</b> by more than 4-fold

Effect of Host–Guest Interactions on the Cloud Points of Neutral Thermosensitive Homopolymers: Poly(<i>N</i>-n-propylmethacrylamide) and Polymers with Similar Structures

We investigated effect of cyclodextrins (CDs) on the cloud point of several thermosensitive polymers that are not ionizable. α-CD increased the cloud point of the poly­(<i>N</i>-n-propylmethacrylamide) (PnPMAm) aqueous solution; by contrast, β-CD or γ-CD did not affect the cloud point of the PnPMAm solution. The cloud point of the PnPMAm solution increased gradually with an increase in the concentration of α-CD. Furthermore, we compared the effect of the CDs on the cloud points of four polymers with similar structures. As for poly­(<i>N</i>-isopropylacrylamide) (PiPAAm), neither α-CD nor β-CD affected its cloud point. On the basis of the effect of the differently sized CDs on the cloud point of five polymers and the corresponding NOESY NMR data, we inferred that steric hindrance by the main chain of PiPAAm might be responsible for the bulky CD being unable to form a complex with the short isopropyl group

MOESM1 of Impairment of NADH dehydrogenase and regulation of anaerobic metabolism by the small RNA RyhB and NadE for improved biohydrogen production in Enterobacter aerogenes

Additional file 1. Figure S1. SDS-PAGE verification of NAD symhetase overexpression in IAM1183 and IAM1183/N. Figure S2. Comparison of the control strain IAM1183 and IAM1183/R carrying pKK102-ryhB-cm plasmid by northern blot analysis

Magnetization and Resistance Switchings Induced by Electric Field in Epitaxial Mn:ZnO/BiFeO₃ Multiferroic Heterostructures at Room Temperature

Electric field induced reversible switchings of the magnetization and resistance were achieved at room temperature in epitaxial Mn:ZnO(110)/BiFeO₃(001) heterostructures. The observed modulation of magnetic moment is ∼500% accompanying with a coercive field varying from 43 to 300 Oe and a resistive switching ratio up to ∼10⁴% with the applied voltages of ±4 V. The switching mechanisms in magnetization and resistance are attributed to the ferroelectric polarization reversal of the BiFeO₃ layer under applied electric fields, combined with the reversible change of oxygen vacancy concentration at the Mn:ZnO/BiFeO₃ interface

AbmV Catalyzes Tandem Ether Installation and Hydroxylation during Neoabyssomicin/Abyssomicin Biosynthesis

Members of the abyssomicin class of natural products are characterized by a novel vinylic bridged ether ring. In this study, in vivo gene inactivation, structure elucidation of the accumulated intermediate abyssomicin 6, and in vitro enzyme assays enabled the identification of a cytochrome P450 enzyme, AbmV. AbmV carries out domino reactions involving bridged ether installation and C-11 hydroxylation during the biosynthesis of neoabyssomicins/abyssomicins in <i>S. koyangensis</i> SCSIO 5802