QPCR was used to determine ratios in normal controls and cohorts of patients with AASV (cohorts described in Supplemental materials and methods)

In each case, results from one representative qPCR plate are shown (all of the raw data are shown in Fig. S1 C). The horizontal bar indicates the mean. P values shown for each cohort indicate comparison of all cases and controls using a stratified Student's test. (A–C) AASV versus control in three independent UK cohorts. (D) Single plate of SLE and AASV, compared using a Student's test. Supplemental materials and methods and Fig. S1 C are available at .<p><b>Copyright information:</b></p><p>Taken from "Copy number of which is associated with systemic lupus erythematosus, correlates with protein expression and immune complex uptake"</p><p></p><p>The Journal of Experimental Medicine 2008;205(7):1573-1582.</p><p>Published online 7 Jul 2008</p><p>PMCID:PMC2442635.</p><p></p

Copy number of which is associated with systemic lupus erythematosus, correlates with protein expression and immune complex uptake-5

plate are shown (all of the raw data are shown in Fig. S1, A and B). The horizontal bar indicates the mean. The P values shown indicate comparison of all cases and controls using a stratified Student's test. (A) UK SLE patients, = 171; UK controls, = 176. (B) Hong Kong SLE patients, = 159; Hong Kong controls, = 150.<p><b>Copyright information:</b></p><p>Taken from "Copy number of which is associated with systemic lupus erythematosus, correlates with protein expression and immune complex uptake"</p><p></p><p>The Journal of Experimental Medicine 2008;205(7):1573-1582.</p><p>Published online 7 Jul 2008</p><p>PMCID:PMC2442635.</p><p></p

Copy number of which is associated with systemic lupus erythematosus, correlates with protein expression and immune complex uptake-1

O a gene, and each column to an individual. Red indicates increased expression compared with PBMC reference; green represents reduced expression. The patient (A in panel B) with no expression is marked with an asterisk. (B) Family tree of the -deficient patient A, showing Mendelian inheritance of the null allele. CN was determined using flow cytometry. (C) Flow cytometry of neutrophils stained for PE-labeled antibody to FcγRIIIb demonstrates reduced surface expression on cells from individuals B, C, and D (with a single copy) compared with individuals E and F, who have two copies. Geometric mean fluorescences were 7, 2,265, 2,241, 2,303, 3,484, and 3,730 for A–F, respectively. (D) Gene dosage of relative to , determined by qPCR, for patient A (no ), her daughter patient B, her son patient C, and her brother patient D (with a single copy), as well as for her husband patient E and her other brother patient F (with two copies of ). (E) qPCR was performed on DNA from all family members whose FcγRIIIb expression had been determined by flow cytometry. Gene dosages of relative to (by qPCR) were significantly higher in those individuals who by flow cytometry were shown to have greater surface expression of FcγRIIIb. The horizontal bar indicates the mean. (F) Delineation of the extent of the deletion in patient A and family members B, C, and E using PCR; , , and are absent. (G) A similar delineation using flow cytometry in patient A. FcγRIIa (neutrophils), FcγRIIb (neutrophils shown, confirmed on B cells, and not depicted) and FcγRIIIa (NK cells) are present (isotype control shaded gray), but FcγRIIIb (neutrophils) is absent. CD59 is expressed on neutrophils, thus GPI linkage is intact.<p><b>Copyright information:</b></p><p>Taken from "Copy number of which is associated with systemic lupus erythematosus, correlates with protein expression and immune complex uptake"</p><p></p><p>The Journal of Experimental Medicine 2008;205(7):1573-1582.</p><p>Published online 7 Jul 2008</p><p>PMCID:PMC2442635.</p><p></p

Copy number of which is associated with systemic lupus erythematosus, correlates with protein expression and immune complex uptake-0

plate are shown (all of the raw data are shown in Fig. S1, A and B). The horizontal bar indicates the mean. The P values shown indicate comparison of all cases and controls using a stratified Student's test. (A) UK SLE patients, = 171; UK controls, = 176. (B) Hong Kong SLE patients, = 159; Hong Kong controls, = 150.<p><b>Copyright information:</b></p><p>Taken from "Copy number of which is associated with systemic lupus erythematosus, correlates with protein expression and immune complex uptake"</p><p></p><p>The Journal of Experimental Medicine 2008;205(7):1573-1582.</p><p>Published online 7 Jul 2008</p><p>PMCID:PMC2442635.</p><p></p