sj-docx-1-ict-10.1177_15347354231226126 – Supplemental material for Hu-Qi-Zheng-Xiao Decoction Inhibits the Metastasis of Hepatocellular Carcinoma Cells by Suppressing the HIF-1α Signaling Pathway to Inhibit EMT, LCSC, and Angiogenic Process

Supplemental material, sj-docx-1-ict-10.1177_15347354231226126 for Hu-Qi-Zheng-Xiao Decoction Inhibits the Metastasis of Hepatocellular Carcinoma Cells by Suppressing the HIF-1α Signaling Pathway to Inhibit EMT, LCSC, and Angiogenic Process by Xuejing Wang, Ling Yin, Mengyin Chai, Buxin Kou, Xiaoni Liu and Xiaojun Wang in Integrative Cancer Therapies</p

Copper(I)-Catalyzed Ligand-Promoted Multicomponent Reactions of Isocyanides, Selenium, Amines, and Iodoarenes: Access to Highly Functionalized Carbamimidoselenoates

A new and facile approach toward highly functionalized carbamimidoseleknoates was developed through the copper­(I)-catalyzed ligand-promoted four-component reaction of isocyanides, selenium, amines, and aryl iodides. The reaction constructed a range of organoselenium compounds containing an isoselenourea skeleton with potential bioactivity directly from selenium powder. The simplicity of method and broad diversity of substrates also highlight this work

Transcriptome Sequencing Analysis Reveals the Regulation of the Hypopharyngeal Glands in the Honey Bee, <i>Apis mellifera carnica</i> Pollmann

<div><p>Transcriptome sequencing has become the main methodology for analyzing the relationship between genes and characteristics of interests, particularly those associated with diseases and economic traits. Because of its role of functional food for humans, commercial royal jelly (RJ) and its production are major research focuses in the field of apiculture. Multiple lines of evidence have demonstrated that many factors affect RJ output by activating or inhibiting various target genes and signaling pathways. Available coding sequences from the Honey Bee Genome Sequencing Consortium have permitted a pathway-based approach for investigating the development of the hypopharyngeal glands (HGs). In the present study, 3573941, 3562730, 3551541, 3524453, and 3615558 clean reads were obtained from the HGs of five full-sister honey bee samples using Solexa RNA sequencing technology. These reads were then assembled into 18378, 17785, 17065, 17105, and 17995 unigenes, respectively, and aligned to the DFCI Honey Bee Gene Index database. The differentially expressed genes (DEGs) data were also correlated with detailed morphological data for HGs acini.</p></div

Cobalt(II)-Catalyzed Isocyanide Insertion Reaction with Sulfonyl Azides in Alcohols: Synthesis of Sulfonyl Isoureas

A Co­(II)-catalyzed isocyanide insertion reaction with sulfonyl azides in alcohols to form sulfonyl isoureas via nitrene intermediate has been developed. This protocol provides a new, environmentally friendly, and simple strategy for the synthesis of sulfonyl isourea derivatives by employing a range of substrates under mild conditions

Venn analysis of the DEGs.

<p>The number of DEGs in the five samples named 2 vs. 1, 3vs. 1, 4vs. 1, and 5vs. 1. Numbers stands for genes expressed in each class. Up- and down- arrow represents the up and down regulated genes, respectively (FDR≤0.001).</p

Validation of selected genes by qRT-PCR.

<p>Gene IDs refer to the corresponding cDNAs from Genbank. qRT-PCR was performed on each sample in triplicate. <i>β-actin</i> was used an endogenous control. Ratio-RPKM used for comparing the difference of gene expression among samples and validation by qRT-PCR to show a certain consistent between the two methods except several inconsistencies. It may be attributed to the experimental errors which caused by the biological replicas deficiency. Only one pooled RNA sequenced for each sample due to the limitations of specimen collection that might cause the experimental errors unpredictably. We will sequence more samples to eliminate the errors in the further research.</p

Sample statistics.

*<p>Agilent Bioanalyzer 2100 RNA integrity number.</p>**<p>The total unigene number in the DFCI Honey Bee Gene Index database is 25007; <a href="http://compbio.dfci.harvard.edu/cgi-bin/tgi/gimain.pl?gudb=honeybee" target="_blank">http://compbio.dfci.harvard.edu/cgi-bin/tgi/gimain.pl?gudb=honeybee</a>.</p>***<p>The unique matched genes from all five samples cover the DFCI database completely.</p

Fémina-gazette : organe cinégraphique hebdomadaire du Fémina-Gaumont

08 juin 19231923/06/08 (A1,N24)-1923/06/08.Appartient à l’ensemble documentaire : PACA

Abundance distribution of unigenes.

<p>The expression level ranges are as follows: [0, 10): low-expression genes; [10, 500): moderate-expression genes; and [500, ∞): high-expression genes. Large differences in abundance were observed for samples 1 and 5.</p

GO enrichment analysis.

<p>The GO terms were classified into 3 categories from the GO database and are ranked by p-value. Green indicates biological processes, red indicates molecular functions, and blue indicates cellular components. (A) Sample 2 vs. 1, (B) Sample 3 vs. 1, (C) Sample 4 vs. 1, (D) Sample 5 vs. 1.</p