Synthesis, structural characterization, <i>in vitro</i> cytotoxicities, and BSA interaction of di-organotin(IV) complexes derived from salicylaldehyde nicotinoyl hydrazone

<p>Two organotin(IV) compounds were synthesized from salicylaldehyde nicotinoyl hydrazone and the corresponding dialkyltin(IV) precursor. Their structures were determined by IR, elemental analysis, NMR, and single crystal X-ray diffraction analysis. Compound <b>1</b> exhibits a distorted trigonal bipyramidal geometry around tin, which is coordinated to the Schiff-base ligand in an enolic tridentate fashion. For <b>2</b>, structural analysis reveals that it is a centro-symmetric trimer, in which the central Sn adopts a six-coordinate octahedral geometry and the other two Sn ions adopt five-coordinate trigonal bipyramidal geometry. <i>In vitro</i> cytotoxicities of the compounds against three human cisplatin-resistant tumor cell lines (A549, HeLa, and MCF-7) were assessed by MTT assay. Further, the interaction of <b>1</b> and <b>2</b> with bovine serum albumin (BSA) has been explored by the titration method with fluorescence quenching spectra and synchronous fluorescence spectra. Studies reveal that di-n-butyltin(IV) complex <b>1</b> with significant antiproliferative effects in the cells shows stronger BSA interaction.</p

Fluorescence Imaging of Intracellular Telomerase Activity Using Enzyme-Free Signal Amplification

A novel enzyme-free signal amplification-based assay for highly sensitive in situ fluorescence imaging and detection of intracellular telomerase activity was developed by using a gold nanoflare probe-triggered mimic-hybridization chain reaction (mimic-HCR) coupled with a graphene oxide (GO) surface-anchored fluorescence signal readout pathway. The nanoflare probe consists of gold nanoparticles (AuNPs) functionalized with a dense shell of nucleic acid sequences by Au–S bond formation. The nucleic acid sequence is composed of three segments: a long thiol-labeled sequence (HS-DNA) and two short sequences (a telomerase primer sequence, “Primer-DNA”, and an FAM-terminated reporter sequence, “Flare-DNA”), both of which are complementary to HS-DNA. The mimic-HCR system is formed by two FAM-modified hairpin sequences that are adsorbed on GO. Upon endocytosis of the AuNP/GO combinatorial probe, the Primer-DNA can be extended by intracellular telomerase at its 3′ end to produce the telomeric repeated sequence, which leads to inner chain substitution and not only releases the Flare-DNA to turn on the fluorescence of FAM but also initiates the subsequent signal amplification and enrichment for the mimic-HCR system anchored on GO. The proposed approach can sensitively detect telomerase activity in living cells, distinguish normal cells from cancer cells, and monitor the change in telomerase activity in response to a telomerase inhibitor

Additional file 1: Figure S1. of The distribution of three candidate cold-resistant SNPs in six minorities in North China

The location of populations. The six minority populations from Hezhen, Daur, Manchu, Korea, Mongolian and Ewenki are located in Heilongjiang Province (around 45°44’N126°39′E). CHB indicates Han Chinese in Beijing (around39°55’N116°27′E). CHS indicates Southern Han Chinese located in Sichuan province (around 30°22’N103°26′E). CDX indicates Chinese Dai in Xishuangbanna (around 22°3’N100°49′E). Greenlandic Inuit population live in area around 71°43’N42°24’W. Northeast Siberian population live in the northeast of Russia (around 78°50’N112°50′E). (DOCX 109 kb

Additional file 2: Table S1. of The distribution of three candidate cold-resistant SNPs in six minorities in North China

The frequencies of three polymorphisms in 11 populations Table S2 The P values for rs7115739 compared between six minorities in northern China and three populations in southern China. (DOCX 16 kb

Additional file 4 of Biomedical association analysis between G2/M checkpoint genes and susceptibility to HIV-1 infection and AIDS progression from a northern chinese MSM population

Supplementary Material 4: Table S4. Association between the tSNPs and HIV-1 infection susceptibility in different subgroups divided by CD4+ T cell count

Additional file 1 of Biomedical association analysis between G2/M checkpoint genes and susceptibility to HIV-1 infection and AIDS progression from a northern chinese MSM population

Supplementary Material 1: Table S1. Distribution of genotypes of tSNPs in cases and control

Additional file 5 of Biomedical association analysis between G2/M checkpoint genes and susceptibility to HIV-1 infection and AIDS progression from a northern chinese MSM population

Supplementary Material 5: Table S5. Gene-gene interaction models, as identified by GMD

Additional file 2 of Biomedical association analysis between G2/M checkpoint genes and susceptibility to HIV-1 infection and AIDS progression from a northern chinese MSM population

Supplementary Material 2: Table S2. Distribution of haplotypes of Chk1 and Cdc25C gene in cases and control

Association of TCF2 rs752010-rs4430796-rs7501939 haplotypes with type 2 diabetes.

<p>Association of TCF2 rs752010-rs4430796-rs7501939 haplotypes with type 2 diabetes.</p

Effects of TCF2 genotypes on the risk of type 2 diabetes under different genetic models.

<p><i>P</i> values and OR values were adjusted for age, sex and log_e BMI.</p