133 research outputs found
The effect of Cytl1 on JAR spheroid adhesion to endometrial cells.
<p>The percentages of JAR spheroid adhesion to HEC-1-A (<b>A</b>) and RL95-2 (<b>B</b>) cells were determined using cell-cell adhesion assays; JAR spheroid adhesion was quantified using CCK8 assays; results are presented as optical density (OD) (<b>C</b>). P, progesterone. Data represent mean±SEM of three independent experiments. *<i>P</i> < 0.05; **<i>P</i> < 0.01.</p
Effects of Cytl1 on endometrial cell proliferation.
<p>Proliferation of HEC-1-A (<b>A</b>) and RL95-2 (<b>B</b>) cells after treatment with different concentrations of Cytl1 or control were determined by CCK-8 assay. Proliferation is represented by optical density (OD) values. P, progesterone. Data represent mean±SEM. *<i>P</i> < 0.05; **<i>P</i> < 0.01.</p
The effect of Cytl1 on the endometrial expression of LIF and HB-EGF.
<p>(<b>A</b>) Reverse -transcription PCR analysis of LIF and HB-EGF mRNA expressions in human endometrial cell lines (HEC-1-A, RL95-2) after treatment with different concentrations of Cytl1. LIF expression in the culture supernatant of HEC-1-A (<b>Ba</b>) and RL95-2 (<b>Bc</b>), and HB-EGF expression in HEC-1-A (<b>Bb</b>) and RL95-2 (<b>Bd</b>) were detected by ELISA. The standard curve prepared according to manufacturer’s instruction was used to determine sample concentrations. P, progesterone. Data represent mean±SEM. *<i>P</i> < 0.05; **<i>P</i> < 0.01.</p
The effects of ovarian hormones on endometrial Cytl1 expression.
<p>(<b>A</b>) Reverse-transcription PCR analysis of Cytl1 mRNA expression in vitro after treatment with ovarian hormones or control (<b>B</b>). Greyscale analysis of Cytl1 expression relative to β-actin expression. Cytl1 protein expression regulated by ovarian hormones or control were analyzed by ELISA in human endometrial cell lines HEC-1-A (<b>C</b>), RL95-2 (<b>D</b>) and the human trophoblastic cell line JAR. (<b>E</b>) Cytl1 expression levels are represented by OD<sub>450</sub> values, and (<b>F</b>) the positive control represents the result of standard sample detected by ELISA. Data shown for each sample are average of results from three array wells. P, progesterone; E, estradiol. Data represent mean±SEM. *<i>P</i> < 0.05; **<i>P</i> < 0.01.</p
Universal Dynamic DNA Assembly-Programmed Surface Hybridization Effect for Single-Step, Reusable, and Amplified Electrochemical Nucleic Acid Biosensing
The traditional sensitive
electrochemical biosensors are commonly
confronted with the cumbersome interface operation and washing procedures
and the inclusion of extra exogenous reagents, which impose the challenge
on the detection simplicity, reliability, and reusability. Herein,
we present the proof-of-principle of a unique biosensor architecture
based on dynamic DNA assembly programmed surface hybridization, which
confers the single-step, reusable, and enzyme-free amplified electrochemical
nucleic acid analysis. To demonstrate the fabrication universality
three dynamic DNA assembly strategies including DNA-fueled target
recycling, catalytic hairpin DNA assembly, and hybridization chain
reaction were flexibly harnessed to convey the homogeneous target
recognition and amplification events into various DNA scaffolds for
the autonomous proximity-based surface hybridization. The current
biosensor architecture features generalizability, simplicity, low
cost, high sensitivity, and specificity over the traditional nucleic
acid-related amplified biosensors. The lowest detection limit of 50
aM toward target DNA could be achieved by hybridization chain reaction-programmed
surface hybridization. The reliable working ability for both homogeneous
solution and heterogeneous inteface facilitates the target analysis
with a robust reliability and reproducibility, also making it to be
readily extended for the integration with the kinds of detecting platforms.
Thus, it may hold great potential for the biosensor fabrication served
for the point-of-care applications in resource constrained regions
Additional file 1: of NextSV: a meta-caller for structural variants from low-coverage long-read sequencing data
Tables S1-S24. Performances of BLASR/PBHoney-Spots, BLASR/PBHoney-Tails, BWA/Sniffles, NGMLR/Sniffles and NextSV on the NA12878 genome and the HX1 genome. (PDF 472 kb
Additional file 1: of Circular stripes were more common in Barrett’s esophagus after acetic acid staining
The features after 2% acetic acid in patients with esophageal epithelial erosion. The esophageal epithelial erosion is always lead by gastroesphageal reflux disease and will impact the mucosa observation after acetic acid staining. (JPEG 666 kb
OAD treatment and control situation in patients treated with diabetes medications.
<p>OAD treatment and control situation in patients treated with diabetes medications.</p
Characteristics of 30 polymorphic microsatellite loci used in this study (FP = forward primer, RP = reverse primer, Ta = annealing temperature).
<p>Characteristics of 30 polymorphic microsatellite loci used in this study (FP = forward primer, RP = reverse primer, Ta = annealing temperature).</p
An Improved and Practical Synthesis of Tranexamic Acid
Tranexamic acid <b>1</b>, a
synthetic antifibrinolytic drug
with the treatment being considered highly cost-effective in many
countries, has been included in the WHO list of essential medicines.
In this paper, we designed the synthesis of <b>1</b> via a novel
seven-step route from the readily available starting material dimethyl
terephthalate, performing with 99.6% purity in 59.2% overall yield.
During the process, we successfully developed a direct and efficient
method for the preparation of key intermediate methyl 4-(acetamidomethyl)Âbenzoate
by one-pot hydrogenation and acylation in acetic anhydride using Ni/Al<sub>2</sub>O<sub>3</sub> as a catalyst. More importantly, it should be
a straightforward and practical way to circumvent the usage of toxic
reagents (CrO<sub>3</sub>, Cl<sub>2</sub>), solvent (CCl<sub>4</sub>), and expensive catalyst (PtO<sub>2</sub>), etc., that plagued the
previous methodologies
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