2 research outputs found
A Bright Fluorescent Probe for H<sub>2</sub>S Enables Analyte-Responsive, 3D Imaging in Live Zebrafish Using Light Sheet Fluorescence Microscopy
Hydrogen
sulfide (H<sub>2</sub>S) is a critical gaseous signaling
molecule emerging at the center of a rich field of chemical and biological
research. As our understanding of the complexity of physiological
H<sub>2</sub>S in signaling pathways evolves, advanced chemical and
technological investigative tools are required to make sense of this
interconnectivity. Toward this goal, we have developed an azide-functionalized <i>O</i>-methylrhodol fluorophore, <b>MeRho-Az</b>, which
exhibits a rapid >1000-fold fluorescence response when treated
with
H<sub>2</sub>S, is selective for H<sub>2</sub>S over other biological
analytes, and has a detection limit of 86 nM. Additionally, the <b>MeRho-Az</b> scaffold is less susceptible to photoactivation than
other commonly used azide-based systems, increasing its potential
application in imaging experiments. To demonstrate the efficacy of
this probe for H<sub>2</sub>S detection, we demonstrate the ability
of <b>MeRho-Az</b> to detect differences in H<sub>2</sub>S levels
in C6 cells and those treated with AOAA, a common inhibitor of enzymatic
H<sub>2</sub>S synthesis. Expanding the use of <b>MeRho-Az</b> to complex and heterogeneous biological settings, we used <b>MeRho-Az</b> in combination with light sheet fluorescence microscopy
(LSFM) to visualize H<sub>2</sub>S in the intestinal tract of live
zebrafish. This application provides the first demonstration of analyte-responsive
3D imaging with LSFM, highlighting the utility of combining new probes
and live imaging methods for investigating chemical signaling in complex
multicellular systems
A Bright Fluorescent Probe for H<sub>2</sub>S Enables Analyte-Responsive, 3D Imaging in Live Zebrafish Using Light Sheet Fluorescence Microscopy
Hydrogen
sulfide (H<sub>2</sub>S) is a critical gaseous signaling
molecule emerging at the center of a rich field of chemical and biological
research. As our understanding of the complexity of physiological
H<sub>2</sub>S in signaling pathways evolves, advanced chemical and
technological investigative tools are required to make sense of this
interconnectivity. Toward this goal, we have developed an azide-functionalized <i>O</i>-methylrhodol fluorophore, <b>MeRho-Az</b>, which
exhibits a rapid >1000-fold fluorescence response when treated
with
H<sub>2</sub>S, is selective for H<sub>2</sub>S over other biological
analytes, and has a detection limit of 86 nM. Additionally, the <b>MeRho-Az</b> scaffold is less susceptible to photoactivation than
other commonly used azide-based systems, increasing its potential
application in imaging experiments. To demonstrate the efficacy of
this probe for H<sub>2</sub>S detection, we demonstrate the ability
of <b>MeRho-Az</b> to detect differences in H<sub>2</sub>S levels
in C6 cells and those treated with AOAA, a common inhibitor of enzymatic
H<sub>2</sub>S synthesis. Expanding the use of <b>MeRho-Az</b> to complex and heterogeneous biological settings, we used <b>MeRho-Az</b> in combination with light sheet fluorescence microscopy
(LSFM) to visualize H<sub>2</sub>S in the intestinal tract of live
zebrafish. This application provides the first demonstration of analyte-responsive
3D imaging with LSFM, highlighting the utility of combining new probes
and live imaging methods for investigating chemical signaling in complex
multicellular systems