111 research outputs found
Extended-spectrum β-lactamase, carbapenemase, and the mcr-1 gene: Is there a historical link?
The plasmid mediated mcr-1 gene encoding for Enterobacteriaceae colistin resistance has been recently identified across five continents. The objective of the present study was to trace historical events concerning the discovery and emergence of the mcr-1 gene along with ESBL and carbapenemase genes since several studies have reported identifying mcr-1 genes among Extended-Spectrum β-Lactamases (ESBL) and/or carbapenemase producing Escherichia coli. A retrospective study reported the identification of the mcr-1 gene in E. coli strains isolated in the 1980s, and this seems to correspond to the first identification of ESBL enzymes. The first discovery of the New Delhi metallo-beta-lactamase-1 (NDM-1) in 2009 was associated with a significant increase in mcr-1 gene prevalence in E. coli strains obtained from food producing animals. We noticed that a historical link has existed between mcr-1, ESBL and carbapenemase genes since the 1980s, and we believe that the re-evaluation of colistin use in livestock needs an overall approach that includes not only colistin use reduction but also the reduction of all antibiotic use
Field trials to evaluate the efficacy of mash feed to reduce Salmonella shedding in swine
The objective of this study owas to evaluate the effect of corn based mash feed as a preharvest intervetion strategy to reduce shedding in pigs herds contaminated by Salmonella spp. In this study, three nurseries previously found contaminated by Salmonella in successive production cycles were selected to evaluate the effect of mash feed
Identification of plasmids in a Salmonella Typhimurium septicemic isolate without the classical 95 kb virulence plasmid
In this study, we report the characterization of plasmids from a Salmonella Typhimurium strain isolated from a septicemic pig. This isolate did not possess the classical 95 kb plasmid associated with virulence, but contained several low molecular weight plasmids
Evaluation of the impact of functional foods on the course of Salmonella infection in piglets
With the aim to improve growth of weaning piglets and to minimize incidence of intestinal diseases, the effect of a combination of functional foods able to stimulate the development of systemic and mucosal immune system and to modulate bacterial populations in the gut was evaluated. In this study, we assessed the impact of functional foods on the course of Salmonella infection in piglets. Piglets from different litters were weaned at 21 days of age and assigned to 1 of the 4 feed additives (8 litters per treatment) as follow: 1-control (CTRL), 2-antibiotic (ATB), 3-cocktail of functional foods (CFF), 4-bovine colostrum + cocktail of functional foods (COL-CFF)
Post weaning diarrhea in pigs: risk factors and non‑colistin‑based control strategies
Post-weaning diarrhea (PWD) is one of the most serious threats for the swine industry worldwide. It is commonly associated with the proliferation of enterotoxigenic Escherichia coli in the pig intestine. Colistin, a cationic antibiotic, is widely used in swine for the oral treatment of intestinal infections caused by E. coli, and particularly of PWD. However, despite the effectiveness of this antibiotic in the treatment of PWD, several studies have reported high rates of colistin resistant E. coli in swine. Furthermore, this antibiotic is considered of very high importance in humans, being used for the treatment of infections due to multidrug-resistant (MDR) Gram-negative bacteria (GNB). Moreover, the recent discovery of the mcr-1 gene encoding for colistin resistance in Enterobacteriaceae on a conjugative stable plasmid has raised great concern about the possible loss of colistin effectiveness for the treatment of MDR-GNB in humans. Consequently, it has been proposed that the use of colistin in animal production should be considered as a last resort treatment only. Thus, to overcome the economic losses, which would result from the restriction of use of colistin, especially for prophylactic purposes in PWD control, we believe that an understanding of the factors contributing to the development of this disease and the putting in place of practical alternative strategies for the control of PWD in swine is crucial. Such alternatives should improve animal gut health and reduce economic losses in pigs without promoting bacterial resistance. The present review begins with an overview of risk factors of PWD and an update of colistin use in PWD control worldwide in terms of quantities and microbiological outcomes. Subsequently, alternative strategies to the use of colistin for the control of this disease are described and discussed. Finally, a practical approach for the control of PWD in its various phases is proposed
Identification and distribution of E. coli virulence gene profiles in an operating swine production network
A number of studies have demonstrated a link between the detection of potentially pathogenic Escherichia coli strains and economic loss in the swine industry. E. coli strains belong to different commensal or pathogenic clonal groups, the latter being characterized by the presence of specific virulence genes
Effect of mash feed on swine intestinal microflora and non-specific immune response.
Pelleting of feed was recommended in the past to reduce the risk of introduction of Salmonella in swine herds. However it was shown more recently that consumption of pelleted feed was associated with an increased probability of seropositivity Furthermore, several studies showed that the prevalence of Salmonella is decreased when mash feed is used. The objective of this study was to evaluate the effect of mash feed as a pre-harvest intervention strategy to prevent Salmonella colonization, to mod1fy of intestinal microflora and to stimulate of the immune system in swine
Protection of pigs against experimental Salmonella Typhimurium infection by use of a single dose subunit slow delivery vaccine
Infections caused by septicemic strains of Salmonella are significant animal health as well as food safety concerns for the North American swine industry. Among the various strategies to control these infections at the herd level, development of vaccines are attractive alternatives. In this study, based on previous studies of immune response to various protems following natural and experimental infections of pigs by Salmonella, we designed a subunit slow delivery vaccine and tested it in an experimental model of infection. The selected immunogenic protein was cloned and purified by chromatography. The purified protein was then incorporated m PLGA (a polymer that is slowly degraded within the animal\u27s gastro-intestinal system) microspheres and given orally once to groups of pigs (n=8) while control animals (n=8) received only PBS. Animals were challenged orally 4 weeks after the vaccmation with 108 cells of a virulent strains of Salmonella Typhimurium. Animals were examined twice a day and climcal signs evaluated using a predetermined scoring grid. Pigs were sacrificed 12 days later and bacterial cultures of vanous organs, electron microscopy and evaluation of lgA response by ELISA were performed. No significant difference was found at bacteriology and ELISA but marked differences in clinical signs were observed between vaccinated and non vaccinated animals. None of vaccmated animals showed fever exceeding 40°C while it was observed in 5 out of 8 non vaccinated Only one of vaccmated pigs showed mild diarrhea while severe diarrhea was observed in all control animals different sizes of microspheres were observed in intestinal crypts of vaccinated animals at electron microscopy. We concluded that this vaccine can protect pigs against clinical signs associated with experimental infection by Salmonella Typhimunum
Impact of use of Tylosin and Virginiamycin on antimicrobial agents resistance profiles of Enterococcus spp. and E. coli isolates from swine in field conditions
Enterococcus spp. and E. coli are recognized as indicator microorganisms for the human and animal intestinal flora, and are also known to be potential reservoirs of antimicrobial resistance genes. Among the various use of antimicrobial agents that can promote antibioresistance, on farm use of growth promoters raises public health concerns. The aim of this study was to evaluate the impact of the use of selected growth promoters on antimicrobial resistance profiles of Enterococcus spp. and E. coli isolates from swine in field conditions. Enterococcus and E. coli isolates obtained from pooled fecal samples of pigs receiving Tylosin (44 ppm, n = 100) and Virginiamycin (22 ppm, n=1 00) were compared to isolates obtained from a control group (n = 1 00) fed without growth promoters, in field conditions. Comparison was done between isolates obtatned in the first week of treatment and isolates obtained 1n the 15th week
Reduction of Salmonella Shedding by Sows during Gestation in Relation to Its Fecal Microbiome
International audiencePork meat is estimated to be responsible for 10-20% of human salmonellosis cases in Europe. Control strategies at the farm could reduce contamination at the slaughterhouse. One of the targeted sectors of production is maternity, where sows could be Salmonella reservoirs. The aim of this study was to assess the dynamics of shedding of Salmonella in terms of variation in both shedding prevalence and strains excreted during gestation in Quebec's maternity sector. The evolution of the fecal microbiota of these sows during gestation was also assessed to detect bacterial populations associated with these variations. A total of 73 sows both at the beginning and the end of the gestation were randomly selected and their fecal matter was analyzed. Salmonella detection was conducted using a method that includes two selective enrichment media (MSRV and TBG). Nine isolates per positive samples were collected. Among the 73 sows tested, 27 were shedding Salmonella. Sows in the first third of their gestation shed Salmonella significantly more frequently (21/27) than those in the last third (6/46) (χ2P < 0.05). The shedding status of 19 of the sows that were previously sampled in the first third of their gestation was followed, this time in the last third of their gestation, which confirmed reduction of shedding. Using 16S rRNA gene sequencing and qPCR, significant differences between the fecal flora of sows at the beginning and the end of the gestation, shedding Salmonella or not and with different parity number were detected. Using MaAsLin, multiple OTUs were found to be associated with the time of gestation, the status of Salmonella excretion and parity number. Some of the identified taxa could be linked to the reduction of the shedding of Salmonella at the end of gestation. In this study, we showed that the level of Salmonella shedding was variable during gestation with significantly higher shedding at the beginning rather than at the end of gestation. We also observed for the first time a significant change in the microbiota during sow gestation and identified interesting taxa which could be linked to a reduced Salmonella shedding
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