Identifikasi Molekuler Pasteurella Multocida Penyebab Pasteurellosis pada Babi di YOGYAKARTA

The purpose of this study was to determine the genetic variation of P.multocida in Yogyakarta with other overseas P. multocida strains. Samples were obtained from necropted intensive pig farming with clinically pneumonia symptoms, specific pulmonary lessions and then followed by histopathological examination and b iochemical characterization of the isolates. Then followed by histopathological examination and biochemical characterization of the isolates. Moleculartest performed by DNA extraction using the QIAGEN QIAamp DNA minikit, amplification of the 16S rRNA gene using forward primer 5' GGA GTG AAC TGC AGC TAA TAC C 3', and reverse primer 5' GTA GGT AAG CTT CGC GTT GTT G 3', electrophoresis, purification and sequencing. Macroscopic and histopathological examination results were analyzed descriptively. Sequencing r esults were analyzed by multiple alignment with other Pasteurella spp. taken from GenBank using the Clustal W software, subsequently analyzed using Neighbour Joining and Maximum Parsimony method that exist in program MEGA version 5.1 The results showed gen etic distance based on 765 nucleotides of 16S rRNA gene of P. multocida isolates from lungs of bronchopneumonia of swine in Yogyakarta at 0%. Filogram based on the nucleotide sequence showed a high similarity between P. multocida isolates from Yogyakarta a nd other isolates from USA, Germany, China, Europe, and Hungaria

Pengaruh Corpus Luteum dan Folikel Dominan terhadap Kualitas Morfologi Oosit Sapi Bali-Timor

Improvement of cattle production can be obtained by the application of feed and breeding technology. One of the breeding technologies that are rapidly growing is in vitro fertilization, which is part of In vitro embryo production The source of oocytes influence the success of in vitro embryo production The quality of the oocyte were determined by Ovarian follicular environment and followed the follicular growth that were characterized by the presence of a dominant follicle (DF) and corpus lutem (CL). The purpose of this study was to determine the influe nced of dominant follicles and corpus luteum on oocyte morphology ofBali Timor cattle, that were collected by aspiration method. Oocytes were obtained postmortem from the ovaries of Bali Timor cows slaughtered at an Oeba slaughterhouse Ovarian then class ified into 3 groups : I ) ovarian with CL without DF; (II) ovarian with DF, without CL; and (III) ovarian without both DF and CL. The results showed that the number of oocytes with good quality from group III (12.5%) was higher than group I (9.83%), but no significant differently (P> 0.05). The number of oocytes from group II that showed several layers of cells and has a cytoplasm (good quality) was very low

Fenotipe Pasteurella Multocida Penyebab Bronchopneumonia pada Babi di YOGYAKARTA

Pasteurella multocida is an important species that are suspected to cause bronchopneumonia in pigs. Prevalence of pneumonia pasteurellosis in slaughter house between 30-80%. The purpose of this study were determined the microorganisms that caused respiratory disorders (cough) or pneumonia in pigs in Yogyakarta area with morphological and biochemical tests. A number of 6 pigs were obtained from some pig farming in Yogyakarta with clinical symptoms of pneumonia were necropted, isolated and identified the cause of pneumonia, followed by histopathological examination of the isolates.The results showed that P. multocida is one of the main causes of bronchopneumonia in pigs in Yogyakarta with clinical symptoms of anorexia, dipsnoea, cough with serous to mucopurulent exudation. Pulmo showed grayish hepatization on dorsocranial, multifocal hemorrhagic and congestion. Histopathological changes found fibrinous bronchopneumonia and catharrhal bronchopneumonia

Gambaran Histopatologi Beberapa Tipe Bronchopneumonia Pada Pulmo Babi Hasil Pemotongan Di Rph Oeba

The aim of this study was to determine the histopathologic changes of bronchopneumonia type in pigs of slaughtered pig from Oeba slaughterhouse associated with P. multocida infection. Samples of pulmo were found from pigs showing respiratoric symptoms, characterized as dyspnoea and presence of nasal exudates that are serous to mucopurulent, obtained from slaughtered pigs in RPH Oeba Kota Kupang. Samples showed pathognomonic lesions of gray hepatized than processed for histopathological studies. The result showed that varied macroscopic changes noted in pulmo samples from suspected infected pigs showed various types of lesions. Lessions observed then classified into two main lessions were acute and chronic. Acute lesions were characterized by edema and hyperemia which then confirmed with congestion and multifocal haemorrhages. Serous to seromucous exudates were observed intrabronchial and bronchioles. In chronic cases, the lesions showed cranioventral consolidation and gray hepatized with demarcated area beetwen consolidated and normal pulmonary area. Histologically, the types of bronchopneumonia categorized into bronchopneumonia suppurativa characterized by coagulative necrosis surrounded by cell infiltration and accumulation of fibrin intraalveolar. The fibrous connective tissue were also observed in the interstitaial space of alveolar around the necrotic area. Other samples showed non-suppurativa fibrinous bronchopneumonia with alveolar exudates dominated by mononuclear cells and accumulation of fibrin intra alveolar. Chronic bronchiolitis also seen with a mixture of mononuclear cells, neutrophils and macrophages that infiltrated into the lumen of the alveoli. A high number of goblet cells were observed in the bronchiolus epithelium, with the thickening of the fibrous tissue around the bronchioles. It can be concluded that there are two major types of lesions which are acute and chronic, with three types of bronchopnemonia namely bronchopneumonia suppurativa, non supuratival bronchopneumonia and chronic bronchiolitis

Karakteristik Pasteurella Multocida Penyebab Pasteurellosis pada Babi di Kota Kupang Provinsi Nusa Tengggara Timur

The number of commercial pigs in East Nusa Tenggara has grown fast with a population of 1,739,481, and has become more potential. However, the mixed farming model has become one of the factor of potentially high in the transmission of disease-causing pathogenic microorganisms. One of the microorganism is Pasteurella multocida which causes pasteurellosis, has been identified in 25% of slaughtered pigs (Maes et al., 2001). One of the clinical symptom due to pasteurellosis in pigs is the occurrence of bronchopneumonia in pulmo and inflammation in various visceral organs, such as the heart and kidneys. the phenotypic characteristization of this bacteria, will be very helpful in designing a comprehensive prevention and treatment programs of pig pasteurellosis. The aim of the research was to determine the characteristics of P. multocida related to pasteurellosis and recording of the disease in Kupang, NTT. This research also find out the phenotipyc characteristics of P. multocida species from pigs and the possibility of transmission among sensitive species. A total of 30 swine lung samples of pulmo were obtained from slaughterhouse in Kupang to carry out this study. Pulmo taken from slaughtered pigs that showed clinical respiratoric symptoms such as dyspnoea and the presence of serous to mucopurulent nasal exudates, and the specific lesions of gray hepatization in pulmo. The collected samples were then processed for histopathological and microbiological studies. Out of the total 30 sample, 15 samples were found to be suspected for pasteurellosis, and 3 samples were successfully confirmed to be positive for Pasteurella multocida. Varied macroscopic changes showed pathognomonic lesions as multifocal hemorrhage and congestion of the pulmonary lobes. Serous to mucopurulent exudate were found in lumen bronchus. Multi lobes grayed hepatization and multifocal hemorrhage were observed in the pulmo. Histopatologic analysis showed three types of pneumonia that were multifocal suppurative bronchopneumonia with neutrophil infiltration into alveoli and bronchioles; non-suppurative pneumonia as fibrinous bronchopneumonia with severe congestion, and chronic bronchiolitis with infiltration of mononuclear cell and thickening of fibrous tissue on bronchioles. Bacterial culture from the samples showed circular, convex and non hemolytic colony on blood agar base. Gram staining's showed Gram negative microorganism with coccoid bipolar structure, which are some of the characteristic of the microorganism.. It was concluded that the samples is having P. multocida infection. Although, some isolate on MacConkey showed lactose fermentation and tolerance to bile salts that were not the nature of the microorganism, isolation and identification from other organs needed to be done, for example from the heart and kidneys, are needed. &nbsp

Gambaran Patologi Anatomi pada Babi Landrace Suspect African Swine Fever (Asf) di Kabupaten Kupang

African Swine Fever (ASF) is a viral disease that attacks pigs and to date has caused many pig deaths in Kupang Regency. ASF is caused by a double-stranded DNA virus from the Asfivirus genus and the Asfarviridae family. This research aims to determine the anatomical pathology of the swine landrace suspect ASF. Organ samples were collected from two male landrace pigs and two female landrace pigs, aged 7 months, from Oeltuah Village, Taebenu District and Tarus Village, Central Kupang District, Kupang Regency, NTT. Clinical examinations were carried out on sick animals that were found during the investigation, then necropsied on the dead animals were carried out and continued with anatomical pathology examinations at the Pathology Laboratory, Faculty of Veterinary Medicine, Nusa Cendana University. Anatomical pathology examinations are carried out by observing changes in the structure and appearance of the organs. The necropsy results showed sub-cutaneous ecchymosis hemorrhage in the abdomen, limbs and ears, gastric, intestinal and hepatic hemorrhage, hemorrhagic lymphadenitis in mesenteric lymph nodes, hyperemic splenomegaly, pteckie hemorrhage in the renal capsule,, multifocal hemorrhage in the renal medulla and pulmonary lobe. Based on the observation of clinical symptoms and changes in anatomical pathology, it can be concluded that the death of pigs was suspected to be caused by the suspect ASF