Management of <em>E. coli</em> Sepsis

E. coli is the most common cause of urinary tract infections (UTIs) in humans and is a leading cause of enteric infections and systemic infections. The systemic infections include bacteremia, nosocomial pneumonia, cholecystitis, cholangitis, peritonitis, cellulitis, osteomyelitis, and infectious arthritis. E. coli is also the leading cause of neonatal meningitis

Indícios de transtornos alimentares em adolescentes / Evidence of eating disorders in adolescents

Os transtornos alimentares (TAs) são síndromes psiquiátricas com etiologia multifatorial, caracterizados por distorções da imagem corporal, associada à insatisfação com o peso e desenvolvimento de comportamentos alimentares específicos, principalmente no público Adolescente. Objetivou-se investigar indícios de TAs em adolescentes na rede pública e privada de ensino no interior de Minas Gerais.Utilizou-se de questionários autoaplicáveis e validados para tendências de anorexia, bulimia, transtorno compulsivo alimentar periódico e vigorexia em adolescentes de ambos os sexos. Tratotou-se de um estudo observacional, analítico e transversal. Incluiram-se no estudo adolescentes com idade entre 12 a 18 anos, alunos de 7° e 8° ano. Quanto aos principais resultados destaca-se a baixa prática de atividade física, maior percentual de índividuos eutróficos pela avaliação do IMC, presença de sobrepeso e baixo peso mais prevalente em meninas, assim como maior tendência para transtornos alimentares como bulimia e anorexia, enquanto nos meninos  maior tendência para vigorexia. A incidência de TAs apresentam complicações multifatoriais a longo prazo e merece mais trabalhos que discorram sobre o assunto. O uso de questionários autoaplícáveis mostrou-se uma ferramenta de baixo custo e ampla abordagem para o rastreamento e identificação desses transtornos na populção adolescente

Optical Density Optimization of Malaria Pan Rapid Diagnostic Test Strips for Improved Test Zone Band Intensity

For the last few decades, the immunochromatographic assay has been used for the rapid detection of biological markers in infectious diseases in humans and animals The assay, also known as lateral flow assay, is utilized for the detection of antigen or antibody in human infectious diseases. There are a series of steps involved in the development of these immuno-chromatographic test kits, from gold nano colloids preparation to nitrocellulose membrane coating (NCM). These tests are mostly used for qualitative assays by a visual interpretation of results. For the interpretation of the results, the color intensity of the test zone is therefore very significant. Herein, the study was performed on a malaria antigen test kit. Several studies have reported the use of gold nanoparticles (AuNPs) with varying diameters and its binding with various concentrations of protein in order to optimize tests. However, none of these studies have reported how to fix (improve) test zone band intensity (color), if different sized AuNPs were synthesized during a reaction and when conjugated equally with same amount of protein. Herein, different AuNPs with average diameter ranging from 10 nm to 50 nm were prepared and conjugated equally with protein concentration of 150 &micro;g/mL with KD = 1.0 &times; 10&minus;3. Afterwards, the developed kits&rsquo; test zone band intensity for all different sizes AuNPs was fixed to the same band level (high) by utilization of an ultraviolet-visible spectrophotometer. The study found that the same optical density (OD) has the same test zone band intensity irrespective of AuNP size. This study also illustrates the use of absorption maxima (&lambda; max) techniques to characterize AuNPs and to prevent wastage of protein while developing immunochromatographic test kits