Plant-Based Foods Biofortified with Selenium and Their Potential Benefits for Human Health

Selenium (Se) is essential for humans. This element is present in more than 25 proteins related to redox processes, and its deficiency is related to the onset of chronic diseases. One way to incorporate Se into the human diet is by consuming plant foods rich in Se. Crop fortification with Se can be achieved through the agronomic practice of biofortification. This chapter discusses dietary sources of inorganic Se (selenate and selenite), organic Se (selenocysteine, selenomethionine, and methylselenocysteine), and bioactive compounds provided by consuming the edible parts of plants as a result of agronomic biofortification. The benefits to human health from consuming selenium-enriched crops due to their biological functions such as antioxidant, anti-inflammatory, and anticarcinogenic are also presented. The intake of Se-enriched plant foods is a growing trend. In addition to providing the daily dose of Se, these Se-enriched vegetables are a functional food option that improves human health due to their content of phytochemical compounds

Efecto del selenito e inulina en la interacción Capsicum annuum L. - Phytophthora capsici en invernadero

Capsicum annuum L. is a crop of great economic importance in Mexico and is severely affected by the wilt caused by Phytophthora capsici. The objective of this work was to determine the effect of selenite and inulin on the incidence of Phytophthora capsici and the growth of serrano pepper plants (Capsicum annuum L.) in greenhouse. Seedlings 30 days after sowing were treated twice with selenite (100 μM) (Se + PHC) or inulin (200 μM) (I + PHC) per application to the base of the plants: 10 days before and at the time of inoculation with P. capsici (PHC). A PHC control and an absolute control without treatment or inoculation were included. After two weeks of treatment, the control seedlings remained without symptoms, while those inoculated with PHC, and those treated with selenite and inulin and inoculated showed a survival rate of 0, 80 and 40%, respectively. The height was higher in the control seedlings. In plants treated with selenite and inoculated a seedling fresh mass was higher than in the PHC and I + PHC, treatments. The viability of the root was similar in the treatments with selenite and inulin and decreased in the seedlings inoculated with PHC. Selenite was more effective than inulin in reducing the incidence of the disease, increasing the fresh mass and maintaining the viability of the plant tissue. The results suggest the potential use of selenite and inulin in the integral management of P. capsici.Keywords: incidence, fructans, plant protection, selenium, symptoms of wiltCapsicum annuum L. es un cultivo de gran importancia económica en México y es severamente afectado por la marchitez ocasionada por Phytophthora capsici. El objetivo de este trabajo fue determinar el efecto del selenito e inulina sobre la incidencia de Phytophthora capsici y el crecimiento de las plantas de chile serrano (Capsicum annuum L.) en invernadero. Plántulas de 30 días después de la siembra se trataron dos veces con selenito (100 µM) (Se+PHC)  o inulina (200 µM) (I+PHC) por aplicación a la base de las plantas: 10 días antes y al momento de la inoculación con P. capsici (PHC). Se incluyó un control PHC y un control absoluto sin tratar ni inocular. Después de dos semanas de tratamiento, las plántulas control permanecieron sin síntomas, mientras que las inoculadas con PHC, y las tratadas con selenito e inulina e inoculadas mostraron un índice de supervivencia de 0, 80 y 40%, respectivamente. La altura fue superior en las plántulas control. En las plantas tratadas con selenito e inoculadas se registró una masa fresca de plántula superior a los tratamientos PHC e I+PHC. La viabilidad de raíz fue similar en los tratamientos con selenito e inulina y disminuyó en las plántulas inoculadas con PHC. El selenito fue más efectivo que la inulina en reducir la incidencia de la enfermedad, incrementar la masa fresca y mantener la viabilidad del tejido vegetal. Los resultados sugieren el uso potencial del selenito y la inulina en manejo integral de P. capsici. Palabras clave: incidencia, fructanos, protección de plantas, selenio, síntoma de marchite

EFECTO DEL ESTRÉS POR COBRE SOBRE EL DESARROLLO DE PLANTAS DE Beta vulgaris L., LA ACUMULACIÓN DE BETACIANINAS Y LA ACTIVIDAD DE GUAIACOL PEROXIDASA.

El objetivo del trabajo fue evaluar el efecto del estrés por cobre sobre el desarrollo de plantas de Beta vulgaris L., la acumulación de betacianinas y la actividad de guaiacol peroxidasa. El cobre se aplicó por infiltración y en cultivos en hidroponía, las concentraciones evaluadas fueron 0.3 (control), 50, 100 y 250 μM de CuSO4 5H2O. La acumulación de betacianinas en las hojas se observó después de dos días de infiltración con 250 μM de cobre. Las hojas más jóvenes presentaron el mayor contenido de betacianinas (100.15 μg eq. betanina disco-1), el cual disminuyó con la edad de las hojas. El cobre se acumuló en las raíces de plantas de 7 semanas tratadas con las diferentes concentraciones de cobre evaluadas en hidroponía, pero no se translocó a las hojas. Sin embargo, el nivel de peroxidación de lípidos incrementó en las hojas jóvenes (7-17 días de edad) después de 3 días de la aplicación del tratamiento con cobre. En las hojas viejas (34-36 días de edad), el nivel de peroxidación de lípidos no cambió durante el período de incubación con cobre. El contenido de clorofilas en las hojas viejas disminuyó un 57% en las plantas tratadas con 250 μM de cobre, sugiriendo que el cobre aceleró la senescencia en estas hojas. La exposición a altas concentraciones de cobre en hidroponía indujo en las hojas jóvenes la actividad de guaiacol peroxidasa. En las hojas viejas, los contenidos de betacianinas fueron de 3 a 5 veces menores que los encontrados en las hojas jóvenes de las plantas incubadas con las mismas concentraciones de cobre. IV La actividad de guaiacol peroxidasa en las hojas viejas incrementó 1.4 veces en el día 3, pero después de 6 días de tratamiento con cobre volvió al nivel encontrado en el control. Lo cual indica que la actividad de guaiacol peroxidasa es parte de la respuesta de defensa al estrés por cobre en hojas. Sin embargo, en el segundo par de hojas de plantas tratadas con 250 μM de cobre se observó una disminución en la actividad de guaiacol peroxidasa que se relacionó con la reducción en el contenido de proteínas. La inhibición del crecimiento de las raíces viejas por cobre se relacionó con la acumulación de cobre. Después de 6 días de tratamiento se observó una disminución en la viabilidad celular en las raíces viejas con todas las concentraciones de cobre evaluadas. La exposición a este metal también promovió la emergencia de nuevas raíces cortas (jóvenes). Las raíces viejas de las plantas control presentaron un contenido basal de betacianinas que se redujo con el incremento en la concentración de cobre. En contraste, las raíces jóvenes registraron el contenido más alto de betacianinas de todas las partes de la planta que se evaluaron. Estos resultados, de forma similar a lo observado en las hojas indican que las betacianinas son acumuladas en órganos con crecimiento activo de plantas de B. vulgaris L. en respuesta al estrés por cobre

Mental Health Problems among COVID-19 Frontline Healthcare Workers and the Other Country-Level Epidemics: The Case of Mexico

COVID-19 frontline healthcare workers (FHCW) are struggling to cope with challenges that threaten their wellbeing. We examine the frequency and predictors of the most frequent mental health problems (MHP) among FHCW during the first COVID-19 peak in Mexico, one of the most severely affected countries in terms of FHCW’s COVID-19 mortality. A cross-sectional survey was conducted between May 8 and August 18, 2020. A total of 47.5% of the sample (n = 2218) were FHCW. The most frequent MHP were insomnia, depression, posttraumatic stress symptoms, and health anxiety/somatization (whole sample: 45.7, 37.4, 33.9, and 21.3%; FHCW: 52.4, 43.4, 40.3 and 26.1, respectively). As compared to during the initial COVID-19 phase, depression and health anxiety/somatization symptoms as well as experiences of grieving due to COVID-19, personal COVID-19 status, and having relatives and close friends with COVID-19 were more frequent during the COVID-19 peak. Obesity, domestic violence, personal COVID-19 status, and grieving because of COVID-19 were included in regression models for main FHCW’s MHP during the COVID-19 peak. In conclusion, measures to decrease other country-level epidemics contributing to the likelihood of COVID-19 complications (obesity) and MHP (domestic violence) as well as FHCW´s probability of COVID-19 infection could safeguard not only their physical but also mental health

International Nosocomial Infection Control Consortiu (INICC) report, data summary of 43 countries for 2007-2012. Device-associated module

We report the results of an International Nosocomial Infection Control Consortium (INICC) surveillance study from January 2007-December 2012 in 503 intensive care units (ICUs) in Latin America, Asia, Africa, and Europe. During the 6-year study using the Centers for Disease Control and Prevention's (CDC) U.S. National Healthcare Safety Network (NHSN) definitions for device-associated health care–associated infection (DA-HAI), we collected prospective data from 605,310 patients hospitalized in the INICC's ICUs for an aggregate of 3,338,396 days. Although device utilization in the INICC's ICUs was similar to that reported from ICUs in the U.S. in the CDC's NHSN, rates of device-associated nosocomial infection were higher in the ICUs of the INICC hospitals: the pooled rate of central line–associated bloodstream infection in the INICC's ICUs, 4.9 per 1,000 central line days, is nearly 5-fold higher than the 0.9 per 1,000 central line days reported from comparable U.S. ICUs. The overall rate of ventilator-associated pneumonia was also higher (16.8 vs 1.1 per 1,000 ventilator days) as was the rate of catheter-associated urinary tract infection (5.5 vs 1.3 per 1,000 catheter days). Frequencies of resistance of Pseudomonas isolates to amikacin (42.8% vs 10%) and imipenem (42.4% vs 26.1%) and Klebsiella pneumoniae isolates to ceftazidime (71.2% vs 28.8%) and imipenem (19.6% vs 12.8%) were also higher in the INICC's ICUs compared with the ICUs of the CDC's NHSN

Effects of pre-operative isolation on postoperative pulmonary complications after elective surgery: an international prospective cohort study an international prospective cohort study

We aimed to determine the impact of pre-operative isolation on postoperative pulmonary complications after elective surgery during the global SARS-CoV-2 pandemic. We performed an international prospective cohort study including patients undergoing elective surgery in October 2020. Isolation was defined as the period before surgery during which patients did not leave their house or receive visitors from outside their household. The primary outcome was postoperative pulmonary complications, adjusted in multivariable models for measured confounders. Pre-defined sub-group analyses were performed for the primary outcome. A total of 96,454 patients from 114 countries were included and overall, 26,948 (27.9%) patients isolated before surgery. Postoperative pulmonary complications were recorded in 1947 (2.0%) patients of which 227 (11.7%) were associated with SARS-CoV-2 infection. Patients who isolated pre-operatively were older, had more respiratory comorbidities and were more commonly from areas of high SARS-CoV-2 incidence and high-income countries. Although the overall rates of postoperative pulmonary complications were similar in those that isolated and those that did not (2.1% vs 2.0%, respectively), isolation was associated with higher rates of postoperative pulmonary complications after adjustment (adjusted OR 1.20, 95%CI 1.05–1.36, p = 0.005). Sensitivity analyses revealed no further differences when patients were categorised by: pre-operative testing; use of COVID-19-free pathways; or community SARS-CoV-2 prevalence. The rate of postoperative pulmonary complications increased with periods of isolation longer than 3 days, with an OR (95%CI) at 4–7 days or ≥ 8 days of 1.25 (1.04–1.48), p = 0.015 and 1.31 (1.11–1.55), p = 0.001, respectively. Isolation before elective surgery might be associated with a small but clinically important increased risk of postoperative pulmonary complications. Longer periods of isolation showed no reduction in the risk of postoperative pulmonary complications. These findings have significant implications for global provision of elective surgical care. We aimed to determine the impact of pre-operative isolation on postoperative pulmonary complications after elective surgery during the global SARS-CoV-2 pandemic. We performed an international prospective cohort study including patients undergoing elective surgery in October 2020. Isolation was defined as the period before surgery during which patients did not leave their house or receive visitors from outside their household. The primary outcome was postoperative pulmonary complications, adjusted in multivariable models for measured confounders. Pre-defined sub-group analyses were performed for the primary outcome. A total of 96,454 patients from 114 countries were included and overall, 26,948 (27.9%) patients isolated before surgery. Postoperative pulmonary complications were recorded in 1947 (2.0%) patients of which 227 (11.7%) were associated with SARS-CoV-2 infection. Patients who isolated pre-operatively were older, had more respiratory comorbidities and were more commonly from areas of high SARS-CoV-2 incidence and high-income countries. Although the overall rates of postoperative pulmonary complications were similar in those that isolated and those that did not (2.1% vs 2.0%, respectively), isolation was associated with higher rates of postoperative pulmonary complications after adjustment (adjusted OR 1.20, 95%CI 1.05–1.36, p = 0.005). Sensitivity analyses revealed no further differences when patients were categorised by: pre-operative testing; use of COVID-19-free pathways; or community SARS-CoV-2 prevalence. The rate of postoperative pulmonary complications increased with periods of isolation longer than 3 days, with an OR (95%CI) at 4–7 days or ≥ 8 days of 1.25 (1.04–1.48), p = 0.015 and 1.31 (1.11–1.55), p = 0.001, respectively. Isolation before elective surgery might be associated with a small but clinically important increased risk of postoperative pulmonary complications. Longer periods of isolation showed no reduction in the risk of postoperative pulmonary complications. These findings have significant implications for global provision of elective surgical care

Risk of COVID-19 after natural infection or vaccinationResearch in context

Background: While vaccines have established utility against COVID-19, phase 3 efficacy studies have generally not comprehensively evaluated protection provided by previous infection or hybrid immunity (previous infection plus vaccination). Individual patient data from US government-supported harmonized vaccine trials provide an unprecedented sample population to address this issue. We characterized the protective efficacy of previous SARS-CoV-2 infection and hybrid immunity against COVID-19 early in the pandemic over three-to six-month follow-up and compared with vaccine-associated protection. Methods: In this post-hoc cross-protocol analysis of the Moderna, AstraZeneca, Janssen, and Novavax COVID-19 vaccine clinical trials, we allocated participants into four groups based on previous-infection status at enrolment and treatment: no previous infection/placebo; previous infection/placebo; no previous infection/vaccine; and previous infection/vaccine. The main outcome was RT-PCR-confirmed COVID-19 >7–15 days (per original protocols) after final study injection. We calculated crude and adjusted efficacy measures. Findings: Previous infection/placebo participants had a 92% decreased risk of future COVID-19 compared to no previous infection/placebo participants (overall hazard ratio [HR] ratio: 0.08; 95% CI: 0.05–0.13). Among single-dose Janssen participants, hybrid immunity conferred greater protection than vaccine alone (HR: 0.03; 95% CI: 0.01–0.10). Too few infections were observed to draw statistical inferences comparing hybrid immunity to vaccine alone for other trials. Vaccination, previous infection, and hybrid immunity all provided near-complete protection against severe disease. Interpretation: Previous infection, any hybrid immunity, and two-dose vaccination all provided substantial protection against symptomatic and severe COVID-19 through the early Delta period. Thus, as a surrogate for natural infection, vaccination remains the safest approach to protection. Funding: National Institutes of Health

Risk of COVID-19 after natural infection or vaccinationResearch in context

Summary: Background: While vaccines have established utility against COVID-19, phase 3 efficacy studies have generally not comprehensively evaluated protection provided by previous infection or hybrid immunity (previous infection plus vaccination). Individual patient data from US government-supported harmonized vaccine trials provide an unprecedented sample population to address this issue. We characterized the protective efficacy of previous SARS-CoV-2 infection and hybrid immunity against COVID-19 early in the pandemic over three-to six-month follow-up and compared with vaccine-associated protection. Methods: In this post-hoc cross-protocol analysis of the Moderna, AstraZeneca, Janssen, and Novavax COVID-19 vaccine clinical trials, we allocated participants into four groups based on previous-infection status at enrolment and treatment: no previous infection/placebo; previous infection/placebo; no previous infection/vaccine; and previous infection/vaccine. The main outcome was RT-PCR-confirmed COVID-19 >7–15 days (per original protocols) after final study injection. We calculated crude and adjusted efficacy measures. Findings: Previous infection/placebo participants had a 92% decreased risk of future COVID-19 compared to no previous infection/placebo participants (overall hazard ratio [HR] ratio: 0.08; 95% CI: 0.05–0.13). Among single-dose Janssen participants, hybrid immunity conferred greater protection than vaccine alone (HR: 0.03; 95% CI: 0.01–0.10). Too few infections were observed to draw statistical inferences comparing hybrid immunity to vaccine alone for other trials. Vaccination, previous infection, and hybrid immunity all provided near-complete protection against severe disease. Interpretation: Previous infection, any hybrid immunity, and two-dose vaccination all provided substantial protection against symptomatic and severe COVID-19 through the early Delta period. Thus, as a surrogate for natural infection, vaccination remains the safest approach to protection. Funding: National Institutes of Health