9 research outputs found

    MOESM10 of Spermatid-specific linker histone HILS1 is a poor condenser of DNA and chromatin and preferentially associates with LINE-1 elements

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    Additional file 10: Table S7. LINE-1 subclass elements identification. Table represents the number of different types of subclasses of LINE-1 repeat elements associated with HILS1 and percentage of HILS1 occupancy to each subclass with respect to the total number of each in the rat genome

    MOESM5 of Spermatid-specific linker histone HILS1 is a poor condenser of DNA and chromatin and preferentially associates with LINE-1 elements

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    Additional file 5: Table S4. ChIP peaks (32731) overlapping with UCSC CpG islands. List of CpG islands were obtained from UCSC table browser and used to find overlaps with 32731 HILS1 ChIP peaks using Bed tools Intersect

    MOESM3 of Spermatid-specific linker histone HILS1 is a poor condenser of DNA and chromatin and preferentially associates with LINE-1 elements

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    Additional file 3: Table S2. Chromosome-wise fold enrichment of HILS1 peaks. Excel file represents the fold enrichment values for HILS1 peaks across all chromosomes of the rat genome

    MOESM6 of Spermatid-specific linker histone HILS1 is a poor condenser of DNA and chromatin and preferentially associates with LINE-1 elements

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    Additional file 6: Table S5. Annotation of the overlapping peaks using HOMER. Overlapping peaks were re-annotated using HOMERv4.7 with newly defined overlap peak lengths and table represents the chromosome-wise number of peaks associated with specific genomic regions like intergenic, intron, exon, 3′UTR, TTS, and promoter-TSS

    MOESM9 of Spermatid-specific linker histone HILS1 is a poor condenser of DNA and chromatin and preferentially associates with LINE-1 elements

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    Additional file 9: Figure 3B. Chr 17-20 and ChrX. Chromosome-wise distribution of rat linker histone HILS1. Each vertical line on the chromosomal map represents location of enriched regions as viewed in UCSC genome browser

    MOESM1 of Spermatid-specific linker histone HILS1 is a poor condenser of DNA and chromatin and preferentially associates with LINE-1 elements

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    Additional file 1: Figure S1. Mass spectrometric analysis of HILS1 IP bands confirms the specificity of HILS1 antibody. Specificity of the antibody raised against CTD of HILS1 was confirmed by the mass spec analysis of the immunoprecipitated bands. Results represent the peptides identified from 25 kDa and ~15kDa bands detected in western blot in both input and immunoprecipitated lane as represented in Figure 5A. Note that the prominent 25kDa band is the full-length form of HILS1, whereas ~15kDa showed different migration in HILS1 IP lane in comparison with input, which is the cleaved product. Coverage represents the percentage of sequence matching with peptides found in the analysis

    MOESM4 of Spermatid-specific linker histone HILS1 is a poor condenser of DNA and chromatin and preferentially associates with LINE-1 elements

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    Additional file 4: Table S3. Chromosome-wise peak length of HILS1 peaks. Excel file represents the length of HILS1 peaks across the chromosomes confirming their broad natur
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