Models and algorithms for multi-crane oriented scheduling method in container terminals

"Multi-crane oriented" is a scheduling method that yard trailers can be shared by different quay cranes. In this paper, two models for this problem are developed. The first one is a model for an inter-ship-based sharing method. In this model, yard trailers can be shared by quay cranes of different ships. To solve the model, a two-phase Tabu search algorithm is designed. The second one is a model for a ship-based sharing method. In this model, yard trailers can only be shared by quay cranes of the same ship. Q-learning algorithm is designed to solve the model. Numerical tests show that the "multi-crane oriented" method can decrease the yard trailers' travel distance, reduce the disequilibrium of different working lines, and thus improve the operation efficiency in container terminals.Container terminals Multi-oriented scheduling method Yard trailer scheduling Q-learning algorithm

Systematic genome editing of the genes on zebrafish Chromosome 1 by CRISPR/Cas9

Genome editing by the well-established CRISPR/Cas9 technology has greatly facilitated our understanding of many biological processes. However, a complete whole-genome knockout for any species or model organism has rarely been achieved. Here, we performed a systematic knockout of all the genes (1333) on Chromosome 1 in zebrafish, successfully mutated 1029 genes, and generated 1039 germline-transmissible alleles corresponding to 636 genes. Meanwhile, by high-throughput bioinformatics analysis, we found that sequence features play pivotal roles in effective gRNA targeting at specific genes of interest, while the success rate of gene targeting positively correlates with GC content of the target sites. Moreover, we found that nearly one-fourth of all mutants are related to human diseases, and several representative CRISPR/Cas9-generated mutants are described here. Furthermore, we tried to identify the underlying mechanisms leading to distinct phenotypes between genetic mutants and antisense morpholino-mediated knockdown embryos. Altogether, this work has generated the first chromosome-wide collection of zebrafish genetic mutants by the CRISPR/Cas9 technology, which will serve as a valuable resource for the community, and our bioinformatics analysis also provides some useful guidance to design gene-specific gRNAs for successful gene editing