Citološka analiza endometrija kod krava s akutnim i kroničnim endometritisom.

The aim of the present study was to evaluate the findings of endometrial cytology in cows with acute and chronic endometritis. For this purpose samples were collected from 217 Brown Swiss and Holstein cows, housed on the Atatürk University Dairy Research Farm between the years 2010 and 2012, and they were stained with Giemsa for cytological examination. In the evaluation, overall 100 cells were counted in the microscopic area and the cells were classified as polymorph nuclear leukocyte, macrophage, lymphocyte and epithelial cells. The cytopathological classification was done according to the percentages of inflammatory cells. Briefly, 126 (58.06 %) samples had extensive inflammatory cells, and of the uterine samples 91 (41.94 %) had normal exfoliation. According to the cellular density results, acute, and chronic and subacute endometritis were described in 68 (31.33 %), 23 (10.60 %) and 35 (16.13 %) cases, respectively. In conclusion, endometrial cytology was found to be an applicable and reliable diagnostic method in diagnosis and diffentiation of acute and chronic endometritis.Cilj istraživanja bio je procijeniti citološke nalaze u endometriju krava s akutnim i kroničnim endometritisom. U tu su svrhu između 2010. i 2012. godine bili prikupljeni uzorci od 217 krava smeđe i holštajnske pasmine, uzgajanih na pokusnoj mliječnoj farmi na Sveučilištu Atatürk. Uzorci su bili obojeni Giemsinim bojenjem. Pri procjeni je u vidnom polju mikroskopa bilo izbrojeno ukupno 100 stanica kojesu razvrstane u polimorfononuklearne leukocite, makrofage, limfocite i epitelne stanice. Citopatološko razvrstavanje bilo je provedeno na osnovi postotka upalnih stanica. Ukratko, upalne stanice bile su ustanovljene u 126 (58,06 %) uzoraka, dok je u 91 (41,94 %) uzorku maternice ustanovljeno normalno ljuštenje stanica. Na osnovi stanične gustoće, akutni endometritis bio je dokazan u 68 (31,33 %) uzoraka, kronični u 23 (10,60 %), a subakutni u 35 (16,13 %) uzoraka. Zaključno, citologija endometrija pokazala se primjenjljivom i pouzdanom metodom u dijagnostici odnosno razlikovanju akutnog od kroničnog endometritisa

The Investigation of Bovine Viral Diarrhoea Virus Antigens with Immunofluorescence and Immunohistochemical Methods in Bovine Abortions

The investigation of bovine viral diarrhoea virus (BVDV) in bovine abortions in Erzurum, Turkey was undertaken with immunofluorescence, immunohistochemistry and histopathological methods. For this purpose, tissue sections from 56 aborted foetuses were examined. At the end of the study, histopathological lesions were observed in 17(30.36%) samples. Hyperaemia in sinusoids and central veins, degeneration and necrosis of some hepatocytes and cellular infiltration in portal areas were main histopathological changes in liver. In brain sections, hyperaemia, degeneration and necrosis of neurons, spongiosis and vasculitis, especially in BVDV positive sections, were prominent. In addition, there was interstitial or catarrhal pneumonia in lung sections. Positive immunofluorescence staining was detected in 8(14.28%) foetal tissues by direct immunofluorescence method. In immunohistochemical staining, 6(10.71%) samples were found positive. Antigenic localisations were observed in the cytoplasm of neuronal cells, neuroglia and leptomeninges in brain; hepatocytes cytoplasm of the liver; apical portion of bronchiolar epithelium and alveolar macrophages of lung sections. It is concluded that BVDV is an effective factor for the cattle abortion in Erzurum, Turkey. (C) 2015 PVJ. All rights reserve