End diastolic pressure (BP_ED) expressed as a percentage change from before cord clamping measured in <i>in utero</i> (•) or <i>ex utero</i> (○) lambs before and after umbilical cord occlusion (designated as time 0).

<p>Data are mean ± SEM. * p&lt;0.05 <i>in utero</i> vs <i>ex utero</i>.</p

Max dP/dt expressed as a percentage change from before cord clamping measured in <i>in utero</i> (•) or <i>ex utero</i> (○) lambs before and after umbilical cord occlusion (designated as time 0).

<p>Data are mean ± SEM. * p&lt;0.05 <i>in utero</i> vs <i>ex utero</i>.</p

Lamb arterial blood gases and physiological parameters at the end of asphyxia.

<p>BP, arterial blood pressure; PaCO₂, partial pressure of carbon dioxide; PaO₂, partial pressure of oxygen; SaO₂, oxygen saturation; BE, base excess. Data are mean ± SD. * p&lt;0.05 <i>in utero</i> vs <i>ex utero</i>.</p><p>Lamb arterial blood gases and physiological parameters at the end of asphyxia.</p

Cerebral oxygen delivery.

<p>Cerebral oxygen delivery in lambs receiving 5 consecutive 3 s sustained inflations (multiple SI; ●), a single 30 s sustained inflation (single SI; ○) or conventional ventilation (no SI; ■) before the onset of ventilation (BV) and with the initiation of ventilation (designated as time 0). Data are mean ± SEM. * p<0.05 single SI vs multiple SI; # p<0.05 single SI vs no SI.</p

Blood vessel profiles.

<p>Number of blood vessel profiles with serum extravasation in the subcortical white matter (A), periventricular white matter (B) and cortical grey matter (C) in lambs that received conventional ventilation (no SI, n = 6; ■), a single 30 s sustained inflation (single SI, n = 6; ○) or 5 consecutive 3 s sustained inflations (multiple SI, n = 5; ●). Data presented as median (IQR). One animal in the multiple SI group was omitted from analysis due to poor tissue quality.</p

Arterial carotid blood flow.

<p>Mean carotid blood flow (CaBF; A), mean systolic CaBF (B), and mean diastolic CaBF (C) in lambs receiving 5 consecutive 3 s sustained inflations (multiple SI; ●), a single 30 s sustained inflation (single SI; ○) or conventional ventilation (no SI; ■) before the onset of ventilation (BV) and with the initiation of ventilation (designated as time 0). Data are mean ± SEM. * p<0.05 single SI vs multiple SI; # p<0.05 single SI vs no SI.</p

Peak dF/dT.

<p>Peak derivative of the carotid arterial blood flow pulse (peak dF/dT) in lambs receiving 5 consecutive 3 s sustained inflations (multiple SI; ●), a single 30 s sustained inflation (single SI; ○) or conventional ventilation (no SI; ■) before the onset of ventilation (BV) and with the initiation of ventilation (designated as time 0). Data are mean ± SEM. * p<0.05 single SI vs multiple SI; # p<0.05 single SI vs no SI.</p

Representative images of blood vessel profiles.

<p>Representative images of blood vessel profiles (indicated by arrowheads) with serum extravasation (indicated by asterisk) in the subcortical white matter of a lamb that received a single 30 s sustained inflation (single SI; A) and 5 consecutive 3 s sustained inflations (multiple SI; B). Representative images of blood vessel profiles (indicated by arrowheads) with no serum extravasation in the subcortical white matter of a lamb that received no sustained inflation (no SI; C). Scale bar represents 90μm.</p

Pulsatility index.

<p>Pulsatility index of the carotid blood flow (PI) in lambs receiving 5 consecutive 3 s sustained inflations (multiple SI; ●), a single 30 s sustained inflation (single SI; ○) or conventional ventilation (no SI; ■) before the onset of ventilation (BV) and with the initiation of ventilation (designated as time 0). Data are mean ± SEM.</p