Additional file 1 of Periviscerokinin (Cap2b; CAPA) receptor silencing in females of Rhipicephalus microplus reduces survival, weight and reproductive output

Additional file 1: Data S1. Nucleotide sequences of the Rhimi-CAP2bR (KC614697.1), two Rhimi-CAP2bR clones (clones #4 and #6) both identical to the original KC614697.1 cDNA, and a 5’-RACE PCR sequence obtained to extend the 5’-UTR region of the R. microplus periviscerokinin receptor. An alignment between the R. microplus genome (isolate Rmic-2018 chromosome 3, ASM1333972v1, NC_051167.1) and the R. microplus periviscerokinin receptor extended RACE-5’-UTR fragment (Accession number OP191701) is provided

Additional file 4 of Periviscerokinin (Cap2b; CAPA) receptor silencing in females of Rhipicephalus microplus reduces survival, weight and reproductive output

Additional file 4: Table S1. dsRNA treatments and respective concentrations used in each replicate of the RNAi experiment

Additional file 5 of Periviscerokinin (Cap2b; CAPA) receptor silencing in females of Rhipicephalus microplus reduces survival, weight and reproductive output

Additional file 5: Table S2. Pictures of ticks showing feeding progression from days 6 to 11 (December 2019), or from days 5 to 14 (July 2021) for all treatments

Additional file 2 of Periviscerokinin (Cap2b; CAPA) receptor silencing in females of Rhipicephalus microplus reduces survival, weight and reproductive output

Additional file 2: Data S2. Nucleotide sequences of the Rhimi-CAP2bR (KC614697.1) and the extended Rhimi-CAP2bR 5’-UTR sequence. The dsRNAs used for Rhimi-CAP2bR silencing are displayed on these sequences. Further, an EMBO-Clustal Omega 1.2.4 multiple sequence alignment between KC614697.1 sequence and R. microplus periviscerokinin receptor RACE 5’-UTR fragment (R-5’CAP2bR) (Accession number OP191701) is provided

Additional file 3 of Periviscerokinin (Cap2b; CAPA) receptor silencing in females of Rhipicephalus microplus reduces survival, weight and reproductive output

Additional file 3: Data S3. NCBI-BLASTn searches to check for possible off-target effects of the Rhimi-CAP2bR dsRNA sequences. Only two short identical sequences to ds956-1109 were found to be ≤ 15 nt in length, which is not sufficient to cause off-target RNAi effects