1 research outputs found
Thermophilic <i>Talaromyces emersonii</i> Flavin Adenine Dinucleotide-Dependent Glucose Dehydrogenase Bioanode for Biosensor and Biofuel Cell Applications
Flavin adenine dinucleotide (FAD)-dependent
glucose dehydrogenase
(GDH) was identified and cloned from thermophilic filamentous fungi Talaromyces emersonii using the homology cloning
method. A direct electron transfer bioanode composed of T. emersonii FAD-GDH and a single-walled carbon nanotube
was produced. Enzymes from thermophilic microorganisms generally have
low activity at ambient temperature; however, the T.
emersonii FAD-GDH bioanode exhibits a large anodic
current due to the enzymatic reaction (1 mA cm<sup>–2</sup>) at ambient temperature. Furthermore, the T. emersonii FAD-GDH bioanode worked at 70 °C for 12 h. This is the first
report of a bioanode with a glucose-catalyzing enzyme from a thermophilic
microorganism that has potential for biosensor and biofuel cell applications.
In addition, we demonstrate how the glycoforms of T.
emersonii FAD-GDHs expressed by various hosts influence
the electrochemical properties of the bioanode