Possibilities of use of means of ICT by preparation of final qualification works of students of pedagogical universities

In article problems of readiness of students and professors of mathematical faculties of pedagogical universities for use of means of information and communication technologies in the organization and carrying out researches within final qualification works of bachelors and masters are analyzedВ статье проанализированы проблемы готовности студентов и преподавателей математических факультетов педагогических университетов к использованию средств ИКТ при организации и проведении исследований в рамках выпускных квалификационных работ бакалавра и магистр

BLOOD LIPOPROTEINS AS A PLATFORM FOR TRANSPORT OF HYDROPHILIC AND HYDROPHOBIC COMPOUNDS

The paper discusses the transport functions of the main classes of blood plasma lipoproteins (LP) that are not associated with the metabolism of lipids that make up their composition. The aim of the study was to study the ability of various plasma LP fractions (very low (VLDL), low (LDL) and high density (HDL)) to interact with certain hydrophilic and hydrophobic compounds and show the role of LP as transport forms of xenobiotics in the organs and tissues of the body. Material and methods. The studies were performed with tritium-labeled cytochalasin B, benzylpenicillin, benzanthracene, benzo(a)pyrene, ultracentrifugation of human plasma LP fractions, column chromatography; in vivo experiments with intravenous injection of LP complexes with tritium-labeled benzanthracene were conducted. Results. The ability of various classes of LP to form complexes with hydrophilic (cytochalasin B, benzylpenicillin) and hydrophobic (benzanthracene, benzo(a)pyrene) compounds is shown by the method of ultracentrifugation. More than 50 % of the radioactivity of hydrophilic compounds in human blood plasma was represented in the composition of the LDL and HDL fractions, and in the composition of the VLDL fractions it was minimal – 6.3 and 5.1 %, respectively. A significant part of cytochalasin and benzylpenicillin was also present in the protein infranatant – 43.6 and 40.9 %, respectively. The distribution in blood plasma for hydrophobic (benzanthracene, benzo(a)pyrene) compounds was different. More than 80 % of the radioactivity was represented in the composition of the LP fractions. The polar protein infranatant contained 16.1 % of the radioactivity of benzantracene and 13.6 % of benzo(a)pyrene. The features of the lipophilic xenobiotics uptake by organs and tissues were shown in vivo experiments with intravenous injection of complexes of LP with tritium-labeled benzanthracene to rats. The highest specific radioactivity was found in the liver and adrenal glands after the intravenous injection of 3H-benzanthracene in the composition of VLDL and LDL. Twice less uptake of the labeled drug was observed in the testis and kidneys. Radioactivity decreased in the series: lungs,adipose tissue, thymus, heart, and spleen. A feature of the use of HDL as a platform for 3H-enzanthracene is the intense accumulation of lipophilic xenobiotics in steroid-producing organs: the adrenal glands and testis. Conclusion. The results obtained allow us to consider the real possibility of using blood plasma PL as transport platforms for hydrophilic and hydrophobic compounds into the cells of organs and tissues

Uptake of cholesterol esters being a part of the different fractions of blood plasma lipoproteins by rats organs and tissues

The paper deals with the functions of the main classes of blood plasma lipoproteins (LP) that are associated with the transport of cholesterol esters included in their composition. The aim of the study was to investigate the features of the uptake of cholesterol esters associated with plasma LP fractions (very low (VLDL), low (LDL), and high density LPs (HDL)) by rat organs and tissues, and to show the participation of various subfractions of HDL (HDL2 and HDL3) as specific cholesterol carriers in the main steroid-producing organs of rats.Material and methods. The in vivo studies with intravenous LP injection of 14C labeled cholesterol oleate (14C-OCh) associated with plasma LP fractions have been carried out.Results. Intravenous injection of a 14C-OCh) in the composition with VLDL led to the maximal mark uptake by the liver. Three times less uptake of labeled cholesterol was observed in the adrenal glands, testes and heart muscle. In other tissues radioactivity gradually decreased in the raw: spleen > lungs > kidneys > thyroid gland and adipose tissue. After the injection of 14C-OCh in the composition of LDL marked predominant uptake of the label by the adrenal glands, testes, and liver. A feature of the use of HDL as a carrier platform for 14C-OCh is the high accumulation of label in steroid-producing organs: the adrenal glands and testis. The dynamics of uptake of 14C-OCh in the composition of HDL by the adrenal glands and testes of rats in different time intervals after injection (30 min, 3, 6 and 12 h) was studied. Adrenal cells actively uptake 14C-OCh from HDL, as a result of which the radioactivity of the tissue increased rapidly and after 30 minutes almost reached its maximum. In contrast to the adrenal glands uptake of the testis was characterized by a gradual increase in radioactivity with a maximum of 6 hours and a rather sharp decrease to 12 hours from the beginning of the experiment. In vitro experiments showed the differences in the effect of HDL2 and HDL3 on the corticosterone production by the adrenal glands of rats.Conclusions. The paper presents the features of uptake of cholesterol esters by organs and tissues of rats depending on the used LP-transporter (VLDL, LDL, HDL). In addition, the results suggest that HDL3 subfraction may be the more preferred source of cholesterol for steroid synthesis in the adrenal cortex of rats compared to HDL2 subfraction

Information availability of official websites of the commissioners for the rights of the child: on the example of the Siberian federal district

В статье объясняется важность информационной доступности сайтов уполномоченных по правам ребенка как составляющей стратегии информирования населения, приводятся результаты анализа сайтов уполномоченных Сибирского федерального округа.The article explains the importance of information accessibility of the websites of the commissioners for the rights of the child as part of a strategy for informing the population, the results of the analysis of the websites of the commissioners of the Siberian Federal District are presented

Metallurgists of the Mining university and development of monetary industry. 245 years of history

Monetary industry combines several stages of metallurgical processes, which are continuously improved with the development of technology and the level of knowledge in the field of non-ferrous metals. The graduates of the Mining Institute, metallurgists of several generations, took part in establishment the Mint and development of technology to produce coins. Since January 24, 1718, when Peter the Great signed the decree «on production of small and large coins ...», the history of the monetary system of Russia and the coining of the first silver rubles began, which subsequently formed the basis of money relations. Twenty-four graduates of the mining and metallurgical departments of the Mining University worked as heads and münzmeister of the Mint. Silver rubles and work of Russian münzmeisters provided financial stability in Tsarist Russia from 1718 to 1917 and laid the foundation for further development of the monetary industry, taking into account new knowledge in the field of enrichment, preparation of polymetallic ores and their melting, and also stamping and processing of precious metals. This is the history of Russia and St. Petersburg, it combined the history of the Mining University, which this year will be 245 years old. It is of scientific interest to restore historical justice and update the methodological knowledge in the field of technology of monetary industry and metallurgical processes

Genetic diversity of hepatitis C virus in Nanaian region, Khabarovsk territory

Examining hepatitis C virus (HCV) genetic diversity is of great practical value in molecular-epidemiological research, development of specific prevention tools and outlining therapeutic strategy.Aim of study is to conduct analysis assessing HCV genetic diversity circulating in the Nanaisky Region population of the Khabarovsk Krai.Materials and methods. Molecular and genetic analysis of 124 blood plasma samples collected from patients with chronic hepatitis C and residing in the Nanaisky Region was conducted.Results. HCV RNA was detected in 84 (67.7±4.2%) plasma samples. HCV genotyping was performed by using AmpliSens — 1/2/3 kit (Central Research Institute of Epidemiology, Moscow, Russian Federation) showing that genotype 3 dominated reaching up to 47.6±5.4% (n = 40). Genotype 1 was detected in 30 patients (35.7±5.2%). In thirteen cases (15.5±3.9%) genotype 2 was identified, whereas in one case (1.2±1.2%) virus genotype was unidentified. Phylogenetic analysis of the nucleotide sequences in HCV NS5B region was performed for 60 HCV RNA-positive samples showing subtype ratio as follows: 1a — 2 (3.3±2.3%), 1b — 23 (38.3±6.3%), 2а — 6 (10.0±3.9%), 2с — 2 (3.3±2.3%), 3а — 27 (45.0±6.4%). Three samples of RF2k/1b recombinant virus were found. A full NS2 gene nucleotide sequence was cloned in order to confirm the recombination event. The results of the study evidence about a need to conduct multi-layered examination of patients with chronic hepatitis C by using current molecular and biologic methods for assigning proper therapy coupled to characteristics of the isolated strains. The data regarding hepatitis C virus molecular and genetic parameters circulating in the Far Eastern Federal District, Russia, are rather limited. Hence, our study would contribute to current understanding of HCV genovariants circulating in territories of the Russian Federation

Apolipoprotein A-I increases the activity of lysosomal glycosidases in the liver of mice with BCG-induced tuberculosis inflammation

This work shows the ability of apolipoprotein A-I to influence the activity of lysosomal glycosidases in the liver of mice in a model of BCG-induced tuberculous inflammation. The aim of the investigation was to study the activity of lysosomal glycosidases in the liver of mice using a model of BCG-induced tuberculous inflammation after intravenous administration of apolipoprotein A-I. Material and methods. The studies were performed on male CBA mice weighing 20-22 g. Disseminated tuberculous inflammation was modeled by a single intraperitoneal injection of 0.5 mg of BCG vaccine. The activity of lysosomal glycosidases was assessed spectrofluorimetrically by the content of hydrolysis products of the corresponding fluorogenic substrates. Results. Tuberculous inflammation led to a pronounced decrease in the activity of lysosomal glycosidases in the liver. Thus, β-galactosidase was decreased 2.3 times, β-glucosidase – 2.8 times, β-glucuronidase – 2.5 times compared with healthy animals. Intravenous injection of apolipoprotein A-I to animals against the background of BCG infection prevented a significant decrease in the enzymatic activity of glycosidases and these values practically did not differ from the control values. Conclusions. Enzymatic activity of lysosomal glycosidases in the group of mice with intravenous administration of apolipoprotein A-I against the background of BCG infection was 1.5–2 times higher than the corresponding indicators in the group of animals with BCG-infection without administration of apolipoprotein A-I, i.e. without treatment