Clustering Results of Synthetic Dataset1.

<p>Clusters are represented by different colors or types of marker. A) 7 actual clusters. B) Clustering result produced by GBHC-TREE has 7 clusters. C) Clustering result produced by GBHC-NODE has 7 clusters. D) Clustering result produced by AE has 7 clusters.</p

Clustering Results of Synthetic Dataset3.

<p>Clusters are represented by different colors or types of marker. A) 7 actual clusters. B) Clustering result produced by GBHC-TREE has 22 clusters. C) Clustering result produced by GBHC-NODE has 12 clusters. D) Clustering result produced by KE has 5 clusters.</p

Additional file 1 of Robust normalization protocols for multiplexed fluorescence bioimage analysis

Appendix. Figure A-1: Column 1 to 4 represent four different cases: first two columns are from histologically normal tissue and the last two are from cancerous tissue of the same patient. Rows 1 to 4 represent pseudo-color images obtained after applying low rank normalization protocols as marked by the experts. Figure A-2: Within class KL-divergence for Patient 2. Within class KL-divergence for second patient after performing phenotyping using different normalization protocols. Figure A-3: Between class KL-divergence for Patient 2. Between class KL-divergence for second patient after performing phenotyping using different normalization protocols. (PDF 341 kb

Clustering Results of Synthetic Dataset2.

<p>Clusters are represented by different colors or types of marker. A) 7 actual clusters. B) clustering result produced by GBHC-TREE has 14 clusters. C) clustering result produced by GBHC-NODE has 37 clusters. D) clustering result produced by KE has 4 clusters.</p

Bayesian hierarchical clustering.

<p>A) A dendrogram represents the merger process of BHC. Each vertical line represents a cluster. A horizontal line connecting between any two vertical lines represents the merger of clusters, where its height is related to the dissimilarity measure between the merged clusters. B) A schematic shows datasets and merged into , where , and are the associated mergers that make , and , respectively. C) BHC prunes the dendrogram at , resulting in the final partition.</p