The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment-0

<p><b>Copyright information:</b></p><p>Taken from "The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment"</p><p>http://www.virologyj.com/content/4/1/120</p><p>Virology Journal 2007;4():120-120.</p><p>Published online 8 Nov 2007</p><p>PMCID:PMC2186317.</p><p></p>cid replacements are indicated with the amino acid position bracketed on the left by the targeted amino acid and on the right by the changed amino acid i.e. Y38A. Carboxyl terminal truncations are indicated by the let (t) following the terminal amino acid of the truncated UL20p. Transmembrane region (TM), Cluster mutant (CL)

The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment-1

<p><b>Copyright information:</b></p><p>Taken from "The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment"</p><p>http://www.virologyj.com/content/4/1/120</p><p>Virology Journal 2007;4():120-120.</p><p>Published online 8 Nov 2007</p><p>PMCID:PMC2186317.</p><p></p>d at 24 hours post infection and tittered on Vero cells (see Materials & Methods). The error bars shown represent the maximum and minimum complementation ratios obtained from three independent experiments, and the bar height represents the average complementation ratio

The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment-3

<p><b>Copyright information:</b></p><p>Taken from "The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment"</p><p>http://www.virologyj.com/content/4/1/120</p><p>Virology Journal 2007;4():120-120.</p><p>Published online 8 Nov 2007</p><p>PMCID:PMC2186317.</p><p></p>Thirty-six hours post-transfection, cells were washed thoroughly, fixed, and processed for confocal microscopy. After permeabilization, rabbit anti-FLAG (α FLAG) mAb was used to detect UL20p, mouse anti-V5 (α V5) epitope was used to detect gK, and sheep aTGN46 mAb was used to identify the TGN. First three rows of the confocal pictures show co-localization of UL20p with gK, while rows 4–6 show colocalization of gK with TGN46

The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment-2

<p><b>Copyright information:</b></p><p>Taken from "The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment"</p><p>http://www.virologyj.com/content/4/1/120</p><p>Virology Journal 2007;4():120-120.</p><p>Published online 8 Nov 2007</p><p>PMCID:PMC2186317.</p><p></p>K) or gB(syn3) mutation. Viral plaques were visualized by immunohistochemistry at 24 hpi

The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment-5

<p><b>Copyright information:</b></p><p>Taken from "The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment"</p><p>http://www.virologyj.com/content/4/1/120</p><p>Virology Journal 2007;4():120-120.</p><p>Published online 8 Nov 2007</p><p>PMCID:PMC2186317.</p><p></p>live conditions with aV5 (gK) mAb for 6 hours. Cells were washed thoroughly, fixed, and processed for confocal microscopy. After permeabilization, antibodies a3 × FLAG, aV5 and aTGN46 were used to identify, UL20p, gK and TGN46, respectively

The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment-6

<p><b>Copyright information:</b></p><p>Taken from "The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment"</p><p>http://www.virologyj.com/content/4/1/120</p><p>Virology Journal 2007;4():120-120.</p><p>Published online 8 Nov 2007</p><p>PMCID:PMC2186317.</p><p></p>cid replacements are indicated with the amino acid position bracketed on the left by the targeted amino acid and on the right by the changed amino acid i.e. Y38A. Carboxyl terminal truncations are indicated by the let (t) following the terminal amino acid of the truncated UL20p. Transmembrane region (TM), Cluster mutant (CL)

The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment-4

<p><b>Copyright information:</b></p><p>Taken from "The herpes simplex virus UL20 protein functions in glycoprotein K (gK) intracellular transport and virus-induced cell fusion are independent of UL20 functions in cytoplasmic virion envelopment"</p><p>http://www.virologyj.com/content/4/1/120</p><p>Virology Journal 2007;4():120-120.</p><p>Published online 8 Nov 2007</p><p>PMCID:PMC2186317.</p><p></p>oroughly, fixed, and processed for confocal microscopy. After permeabilization, antibodies a3xFLAG, aV5 and aTGN46 were used to identify, UL20p, gK and TGN46, respectively

Herpes simplex virus type-1(HSV-1) oncolytic and highly fusogenic mutants carrying the NV1020 genomic deletion effectively inhibit primary and metastatic tumors in mice-0

Rnal repeat (IR) regions. (b) Approximate locations of the gB and gK genes. (c) An expansion of the inverted repeat region showing the approximate locations of UL54, UL55, UL56, α 0, γ34.5, α 4, α 22 and US2 genes. (d) Schematic of the DNA fragment cloned into plasmid pJM-R, which was used for insertion of the HcRed gene cassette into the viral genome in place of the NV1020 genomic deletion as described in Materials and Methods.<p><b>Copyright information:</b></p><p>Taken from "Herpes simplex virus type-1(HSV-1) oncolytic and highly fusogenic mutants carrying the NV1020 genomic deletion effectively inhibit primary and metastatic tumors in mice"</p><p>http://www.virologyj.com/content/5/1/68</p><p>Virology Journal 2008;5():68-68.</p><p>Published online 2 Jun 2008</p><p>PMCID:PMC2453120.</p><p></p

Herpes simplex virus type-1(HSV-1) oncolytic and highly fusogenic mutants carrying the NV1020 genomic deletion effectively inhibit primary and metastatic tumors in mice-1

S were visualized 48 hr post infection by immunohistochemistry and photographed with a phase contrast microscope. Vero (g) and 4T1 (h) cells were infected with OncdSyn virus. Viral plaques were photographed 48 hr postinfection with a fluorescent microscope.<p><b>Copyright information:</b></p><p>Taken from "Herpes simplex virus type-1(HSV-1) oncolytic and highly fusogenic mutants carrying the NV1020 genomic deletion effectively inhibit primary and metastatic tumors in mice"</p><p>http://www.virologyj.com/content/5/1/68</p><p>Virology Journal 2008;5():68-68.</p><p>Published online 2 Jun 2008</p><p>PMCID:PMC2453120.</p><p></p

Herpes simplex virus type-1(HSV-1) oncolytic and highly fusogenic mutants carrying the NV1020 genomic deletion effectively inhibit primary and metastatic tumors in mice-5

Sentative stained sections are shown for PBS (c, d) and OncdSyn (e, f) groups at 40× (c, e) and 100× (d, f) magnifications. Metastatic foci are represented by arrows (c, d).<p><b>Copyright information:</b></p><p>Taken from "Herpes simplex virus type-1(HSV-1) oncolytic and highly fusogenic mutants carrying the NV1020 genomic deletion effectively inhibit primary and metastatic tumors in mice"</p><p>http://www.virologyj.com/content/5/1/68</p><p>Virology Journal 2008;5():68-68.</p><p>Published online 2 Jun 2008</p><p>PMCID:PMC2453120.</p><p></p