SP503-I Insects: Identifying the Flatheaded Appletree Borer and Other Buprestid Beetle Species in Tennessee

Version 2.

SP503-I Insects: Identifying the Flatheaded Appletree Borer and Other Buprestid Beetle Species in Tennessee

Version 2.

SP503-I Insects: Identifying the Flatheaded Appletree Borer and Other Buprestid Beetle Species in Tennessee

Version 2.

The Longhorned Beetles (Coleoptera: Cerambycidae) of Tennessee: Distribution of Species, Seasonal Adult Activity, and New State Records

Efforts to document species of longhorned beetles (Coleoptera: Cerambycidae) occurring in Tennessee have not been updated since 1973. To address this knowledge gap, institutional, research, and private collections in Tennessee were reviewed to provide faunal distribution assessments and seasonal activity data for the cerambycid beetle species active in Tennessee. Examinations of 9,918 specimens and records yielded a list of 230 cerambycid beetle species within 5 subfamilies. Twenty-seven species are reported as new state records from Tennessee. Adult seasonal activity data that were recorded on specimen labels are presented. Where available, notes on collection method, adult resources, and larval host plants are provided for species within a supplementary table. Supplemental figures report the distribution for species collected across the state and from 85 of the 95 Tennessee counties, as well as the ecoregions from which each species is reported. The bias-corrected Chao1 species richness estimator predicts another 11 species remain to be identified across the state. Future collection efforts in the Central Appalachian, Mississippi Alluvial and Valley Loess Plains, Southeastern Plains, and western portions of the Interior Plateau ecoregions could yield additional new state records. Developmental host and adult resource plants, collection methods, as well as regional collection notes from adjacent states are discussed for several additional candidate longhorned beetle species

Molecular Identification of Synanthedonini Members (Lepidoptera: Sesiidae) Using Cytochrome Oxidase I

Many North American sesiid moths within Synanthodonini have been studied extensively because their feeding activity can cause detrimental economic and esthetic impacts to many commercially important ornamental and native plant species. Recent discoveries of nonnative clearwing moth pest introductions [e.g., Synanthedon myopaeformis (Borkh.)], reinforce the need for reliable and accurate molecular diagnostic tools that can be used by nontaxonomic experts, particularly when juvenile life stages are recovered from infested host-plant tissues. Cytochrome oxidase I (cox I) previously has been used to successfully identify species and resolve species complexes. In this study, the cox I phylogeny inferred from sequences generated from 21 species of sesiid moths classified within Synanthedonini confirms the close evolutionary relationship between sesiid species. As other authors have suggested in previous works, we observed that Synanthedon rileyana H. Edwards appears atypical for the genus, as it paired with Carmenta bassiformis (Walker) one node removed from, but not sister to, a large well-supported Synanthedon-rich clade. Sannina uroceriformis Walker and Podosesia MXöschler were observed nested deeply within the aforementioned well-supported clade (posterior probability [PP] of clade = 100) comprised of all Synanthedon species sampled, except S. rileyana. Placement of these two taxa conflicts with results from previous morphological studies. These placements were immune from repeated attempts to delete perceived nearby long branches within the data set. Despite these few conflicts and overall low statistical support for most interspecific and higher relationships, our data suggest that all species examined possess unique genetic signatures that lend themselves to accurate identification of all life history stages of these clearwing pests

Sciadopitys verticillata Resin: Volatile Components and Impact on Plant Pathogenic and Foodborne Bacteria

Sciadopitys verticillata (Sv) produces a white, sticky, latex-like resin with antimicrobial properties. The aims of this research were to evaluate the effects of this resin (Sv resin) on bacterial populations and to determine the impact of its primary volatile components on bioactivity. The impact of sample treatment on chemical composition of Sv resin was analyzed using Fourier transform infrared spectroscopy (FTIR) coupled with principal component analysis. The presence and concentration of volatiles in lyophilized resin were determined using gas chromatography/mass spectrometry (GC/MS). Changes in bacterial population counts due to treatment with resin or its primary volatile components were monitored. Autoclaving of the samples did not affect the FTIR spectra of Sv resin; however, lyophilization altered spectra, mainly in the CH and C=O regions. Three primary bioactive compounds that constituted \u3e90% of volatiles (1R-α-pinene, tricyclene, and β-pinene) were identified in Sv resin. Autoclaved resin impacted bacterial growth. The resin was stimulatory for some plant and foodborne pathogens (Pseudomonas fluorescens, P. syringae, and Xanthomonas perforans) and antimicrobial for others (Escherichia coli, Bacillus cereus, Agrobacterium tumefaciens, and Erwinia amylovora). Treatment with either 1R-α-pinene or β-pinene reduced B. cereus population growth less than did autoclaved resin. The complex resin likely contains additional antimicrobial compounds that act synergistically to inhibit bacterial growth

Seasonal Flight Activity and Distribution of Metallic Woodboring Beetles (Coleoptera: Buprestidae) Collected in North Carolina and Tennessee

Distribution records and seasonal flight activity information for metallic woodboring beetle (Coleoptera: Buprestidae) species have not been compiled for North Carolina and Tennessee. Institutional, research, and private collections in North Carolina and Tennessee were reviewed to provide seasonal activity data of 5 subfamilies of buprestid beetle species. Label information was checked for 15,217 specimens of 135 species collected between 1901 and 2013 (North Carolina) and between 1934 and 2013 (Tennessee). These collections provided data on adult seasonal activity and county records for 121 species (4,467 specimens) and 105 species (10,750 specimens) from North Carolina and Tennessee, respectively. Two species, Agrilus carpini Knull and A. pensus Horn, are reported as New State Records for North Carolina. The data reveal key geographic areas in both states where few to no collections have been made, highlighting opportunities to validate species distributions and locations where future collecting efforts can be matched with the occurrence of larval and adult host plant resources. Seasonal activity records will inform future biosurveillance efforts for invasive and endemic pests and facilitate predictions of buprestid species that are likely to be active within the hunting flight season of Cerceris fumipennis (Say) (Hymenoptera: Crabronidae) wasps. Activity periods of the buprestids also can focus the management of selected economic pest species to times of the year when treatment efforts, particularly through use of contact insecticides, are likely to be most effective

Map Supplements for The Metallic Woodboring Beetles (Coleoptera: Buprestidae) of Tennessee

Following compilation of distribution records and seasonal flight activity information for 106 metallic wood-boring beetle (Coleoptera: Buprestidae) species for Tennessee, maps were updated to provide county-level collection notations for 10,543 published records and specimen collections made from 1934 to 2013. County collection tallies across the ecoregions in Tennessee are also presented. Maps for individual species highlight gaps in key geographic areas where specimens have not been collected and can be valuable for future biosurveillance, monitoring and management efforts for these economically and ecologically important insects

CONSUMERS' VALUATION OF DISEASE-RESISTANT NURSERY STOCK: A CASE STUDY OF DOGWOODS

This article summarizes a study of consumers' willingness to pay (WTP), in urban areas in Tennessee, Mississippi, and Michigan, for a powdery mildew resistant dogwood tree. Powdery mildew is a disease affecting flowering dogwoods that can limit growth, detract from the appearance, and may cause plant decline and death. Study objectives were to provide information about consumers' WTP and to identify potential marketing strategies for the introduction of the disease-resistant tree. On average, survey respondents indicated they are willing to pay a $13.35 premium for a flowering dogwood tree which is resistant to powdery mildew. Regression results led to inferences that the presence of dogwoods in a respondent's yard, presence of dogwoods infected with powdery mildew in a yard, landscape expenditures, presence of flower beds, landscape satisfaction, criteria for selecting plants and trees, retail outlets where respondents shop for landscape materials, geographic location, and income had significant effects on the WTP. Marketing implications include the need to provide information at the point of sale, to place the trees near flowering plants at outlets, and to interact with shoppers to determine characteristics of their yards

CONSUMERS' VALUATION OF DISEASE-RESISTANT NURSERY STOCK: A CASE STUDY OF DOGWOODS

This article summarizes a study of consumers' willingness to pay (WTP), in urban areas in Tennessee, Mississippi, and Michigan, for a powdery mildew resistant dogwood tree. Powdery mildew is a disease affecting flowering dogwoods that can limit growth, detract from the appearance, and may cause plant decline and death. Study objectives were to provide information about consumers' WTP and to identify potential marketing strategies for the introduction of the disease-resistant tree. On average, survey respondents indicated they are willing to pay a $13.35 premium for a flowering dogwood tree which is resistant to powdery mildew. Regression results led to inferences that the presence of dogwoods in a respondent's yard, presence of dogwoods infected with powdery mildew in a yard, landscape expenditures, presence of flower beds, landscape satisfaction, criteria for selecting plants and trees, retail outlets where respondents shop for landscape materials, geographic location, and income had significant effects on the WTP. Marketing implications include the need to provide information at the point of sale, to place the trees near flowering plants at outlets, and to interact with shoppers to determine characteristics of their yards.contingent valuation, disease resistance, dogwood, marketing, maximum likelihood, nursery, powdery mildew, Crop Production/Industries, Marketing,