The Comparison of Autoclave, Povidone Iodine 10%, and Boiling Toward Radiological Union Rates and Bacterial Culture

Introduction.There are some controversies about the appropriate management of contaminated bone graft. The purpose of this study was to determine the which one is the most optimal method of decontaminating bone from which often used. Regarding to radiological union rates and microbiological culture.Materials and methods.Twenty eight samples of femur were discarded from 28 male Sprague dawley rats divided into 4 groups of treatment. The bone samples were uniformly contaminated by broth containing Staphylococcus aureus, except the Saline group. The bone samples in each group underwent four different decontamination procedures: Normal Saline, autoclave, Povidone iodine 10%, and boiling, and reimplanted with Kirchner wire fixation.Results.After 8 weeks reimplantation, Radiological examination showed that povidone iodine had higher radiological union rate and lower infection rate compared autoclave, boiling, and normal saline. There was no statistically significant difference in radiological union rate and bone graft infection rate among autoclave, povidone iodine, and boiling, except between povidone iodine and saline group.Conlusions.Decontamination method should have minimal deleterious effect to cell viability and no infection risk. In this study, we found an easily accessible and clinically relevant method of decontaminating contaminated bone by using povidone iodine 10%. Decontamination of cortical bone sample by povidone iodine 10% offers the best balance between lower infection risk of contaminated bone and better union rate

Uji Kepekaan Bakteri Yang Diisolasi Dari Urin Penderita Infeksi Saluran Kemih (Isk) Terhadap Beberapa Antibiotika Pada Periode Maret–juni 2008

Urinary tract infections (UTI) was an inflammatory response of the urethra whenattacked by microorganisms. This disease can happen to people at all ages beginningwith asymptomatic to moderately symptomatic infections. Thus far, antibiotic treat-ment was the best for curing the UTI although the chances for being resistance tothe antibiotics were also high. Resistance to gram negative bacteri,a in particular,often occur with the antibiotics treatment. Our study at the Faculty of Medicine,University of Indonesia (UI), Bacterial isolation from patient urine sample wasperformed in the microbiology laboratory of UI. A total of 50 urine samples werecollected from X patients volunteered in our study for bacterial isolation, however,only 23 bacterial isolates were successfully obtained. Study was carried out to moni-tor the susceptibility of bacterial isolate towards several types of antibiotics (ofloksazim,amoxyicillin, fosfomisin and sefepim) using Cakram disffusion method. Study re-sults demonstrated that susceptibility of oflokazim to both gram positive and nega-tive bacteria was very low while amoxycillin showed desecding trend of efficacytowards all types of bacteria. Fosfomisin and sefepim, on the other hand, demon-strated strong susceptibility to both gram positive and negative bacteria found in theisolates. Furthermore, it is very interesting to observe ofloksazim was resistant to gram postivie and negative bacteria. Collectively, these research findings stronglyillustrated the susceptibility patten and resistance scale of baterial isolates towards various antibiotic tested in the study