Immunoreactivity for a calmodulin-dependent protein kinase is selectively increased in macaque striate cortex after monocular deprivation

Immunocytochemical methods were used to localize type II Ca2+/calmodulin-dependent protein kinase in the macaque primary visual cortex. Neurons that stain for the kinase include both pyramidal and nonpyramidal cells and they appear to form a subset of cortical neurons. They are densely packed in layers II and IVB, somewhat more sparse in layers III, IVCß, and VI, and nearly absent in layer V. In normal animals the distribution of kinase-positive cells within each layer is relatively uniform. However, in animals in which one eye is removed 7-14 days before sacrifice or sutured shut for 9 or 11 weeks, the cells in layer IVCß are divided into alternating lightly and darkly stained bands. Comparison of immunocytochemically stained sections with adjacent sections stained for the mitochondrial enzyme, cytochrome oxidase, reveals that the kinase staining increases in ocular dominance columns originally driven by the removed or closed eye. These findings suggest that either the concentration of type II Ca2+/calmodulin-dependent protein kinase or its accessibility to the antibody probe increases dramatically and selectively in neurons of macaque primary visual cortex that have been deprived of their normal visual input. This may indicate that changing levels of activity in cortical neurons can alter their regulatory machinery

Regulation of the Neuron-specific Ras GTPase-activating Protein, synGAP, by Ca2+/Calmodulin-dependent Protein Kinase II

synGAP is a neuron-specific Ras GTPase-activating protein found in high concentration in the postsynaptic density fraction from mammalian forebrain. Proteins in the postsynaptic density, including synGAP, are part of a signaling complex attached to the cytoplasmic tail of the N-methyl-D-aspartate-type glutamate receptor. synGAP can be phosphorylated by a second prominent component of the complex, Ca2+/calmodulin-dependent protein kinase II. Here we show that phosphorylation of synGAP by Ca2+/calmodulin-dependent protein kinase II increases its Ras GTPase-activating activity by 70-95%. We identify four major sites of phosphorylation, serines 1123, 1058, 750/751/756, and 764/765. These sites together with other minor phosphorylation sites in the carboxyl tail of synGAP control stimulation of GTPase-activating activity. When three of these sites and four other serines in the carboxyl tail are mutated, stimulation of GAP activity after phosphorylation is reduced to 21 ± 5% compared with 70-95% for the wild type protein. We used phosphosite-specific antibodies to show that, as predicted, phosphorylation of serines 765 and 1123 is increased in cultured cortical neurons after exposure of the neurons to the agonist N-methyl-D-aspartate

Asymptotic behaviour and numerical approximation of optimal eigenvalues of the Robin Laplacian

We consider the problem of minimising the $n^{th}-$eigenvalue of the Robin Laplacian in $\mathbb{R}^{N}$. Although for $n=1,2$ and a positive boundary parameter $\alpha$ it is known that the minimisers do not depend on $\alpha$, we demonstrate numerically that this will not always be the case and illustrate how the optimiser will depend on $\alpha$. We derive a Wolf-Keller type result for this problem and show that optimal eigenvalues grow at most with $n^{1/N}$, which is in sharp contrast with the Weyl asymptotics for a fixed domain. We further show that the gap between consecutive eigenvalues does go to zero as $n$ goes to infinity. Numerical results then support the conjecture that for each $n$ there exists a positive value of $\alpha_{n}$ such that the $n^{\rm th}$ eigenvalue is minimised by $n$ disks for all $0<\alpha<\alpha_{n}$ and, combined with analytic estimates, that this value is expected to grow with $n^{1/N}$

Study of 42 and 85 GHz coupled cavity traveling-wave tubes for space use

Designs were formulated for four CW, millimeter wavelength traveling-wave tubes having high efficiency and long life. Three of these tubes, in the 42 to 44 GHz frequency region, develop power outputs of 100 to 300 watts with overall efficiencies of typically 45 percent. Another tube, which covers the frequency range of 84 to 86 GHz, provides a power output of 200 watts at 25 percent efficiency. The cathode current density in each design was 1A/sq cm. Each tube includes: metal-ceramic construction, periodic permanent magnet focusing, a two step velocity taper, an electron beam refocusing section, and a radiation cooled three-stage depressed collector. The electrical and mechanical design for each tube type is discussed in detail. The results of thermal and mechanical analyses are presented

Optimal solutions for complex design problems: Using isoperformance software for human factors trade offs

A major application of isoperformance is as a trade-off methodology of the three major drivers of system design; equipment, training variables, and user characteristics. The flexibility of isoperformance allows each of these three components to be nearly any rational variation. For example, aptitude may be military Armed Forces Qualification Testing (AFQT) categories, cutoff scores within a selection procedure, or simply dichotomizing high and low scorers (pass/fail). Equipment may be new versus old, 'smart' versus dumb, high versus low resolution, etc. Training may be short versus long or varieties of media types (lecture versus CAI/CBI versus self-paced workbooks). In its final computerized form isoperformance lets the user set an operational level of performance (e.g., a jet pilot in a simulated emergency must take prescribed corrective action and clear the plane in several seconds, pilot astronauts will check out all shuttle flight systems within 30 minutes, or Mission Specialists must handle sucdessfully a required number of job elements). At this point the computer program guides the user through any requested trade-offs of the three components while maintaining the specified operational level of performance through isoperformance curves. A demonstration of the computer program is currently available

Multiplexed Memory-Insensitive Quantum Repeaters

Long-distance quantum communication via distant pairs of entangled quantum bits (qubits) is the first step towards more secure message transmission and distributed quantum computing. To date, the most promising proposals require quantum repeaters to mitigate the exponential decrease in communication rate due to optical fiber losses. However, these are exquisitely sensitive to the lifetimes of their memory elements. We propose a multiplexing of quantum nodes that should enable the construction of quantum networks that are largely insensitive to the coherence times of the quantum memory elements.Comment: 5 pages, 4 figures. Accepted for publication in PR

Coplanar Circumbinary Debris Disks

We present resolved Herschel images of circumbinary debris disks in the alpha CrB (HD139006) and beta Tri (HD13161) systems. We find that both disks are consistent with being aligned with the binary orbital planes. Though secular perturbations from the binary can align the disk, in both cases the alignment time at the distances at which the disk is resolved is greater than the stellar age, so we conclude that the coplanarity was primordial. Neither disk can be modelled as a narrow ring, requiring extended radial distributions. To satisfy both the Herschel and mid-IR images of the alpha CrB disk, we construct a model that extends from 1-300AU, whose radial profile is broadly consistent with a picture where planetesimal collisions are excited by secular perturbations from the binary. However, this model is also consistent with stirring by other mechanisms, such as the formation of Pluto-sized objects. The beta Tri disk model extends from 50-400AU. A model with depleted (rather than empty) inner regions also reproduces the observations and is consistent with binary and other stirring mechanisms. As part of the modelling process, we find that the Herschel PACS beam varies by as much as 10% at 70um and a few % at 100um. The 70um variation can therefore hinder image interpretation, particularly for poorly resolved objects. The number of systems in which circumbinary debris disk orientations have been compared with the binary plane is now four. More systems are needed, but a picture in which disks around very close binaries (alpha CrB, beta Tri, and HD 98800, with periods of a few weeks to a year) are aligned, and disks around wider binaries (99 Her, with a 50 yr period) are misaligned, may be emerging. This picture is qualitatively consistent with the expectation that the protoplanetary disks from which the debris emerged are more likely to be aligned if their binaries have shorter periods.Comment: accepted to MNRA

Condom Use among Young African American Men: Implications for Planning Interventions

Condom Use among Young African American Men: Implications for Planning Interventions Sexually transmitted diseases, including HIV, continue to present significant public health problems affecting young people in the United States, especially African Americans. While African Americans make up about 12% of the U.S. population, in 2010 they accounted for 44% of new HIV infections in 2010 and 48% of all persons living with AIDS in 2007. The 2010 data shows that of these new cases, 38% occurred among African American males ages 13-24 years old. Correct condom use remains a challenge in this population and efforts to increase condom use among minority males has been a formidable challenge. This paper reports the results of formative research conducted in order to guide the development of an intervention to increase consistent, effective condom use for young African American males. Methods: A snowball sampling approach was used to recruit participants. African American males, ages 18-24, who self-reported as sexually active were eligible to participate in one of four focus groups or one of six individual interviews. All study events were conducted at community locations. Each event was audiotaped and notes were taken. Analysis was performed using using NVivo-9. The coding strategy included emic and etic codes and a coding tree was developed which was used to identify themes. Results: A total of 36 African American males between the ages of 18-24 (mean 20.7 years) took part. In general, participants felt condom use was highly influenced by contextual factors including partner interest, partner communication, length of relationship and trust. Condom use was also influenced by a sense of invincibility and being caught up in the moment. Notably, most sexual activity occurred outside of a relationship, most often within the party scene or as quickly arranged hook-ups. Analysis: In order to ensure maximum impact on the development of the intervention, the results from this formative phase were viewed through the Transtheoretical Model of Behavior Change (TTM) and most participants would be described as being in the pre-contemplation or contemplation stages of behavioral change. While all participants expressed some understanding of the risks of unprotected sex, many did not connect risks to consequences. While the data did not indicate that condom use behavior was likely to change in the short-term (less than 6 months), several participants were contemplating making a change. Discussion: The snowball sampling approach allowed us to understand the participants’ social network and allowed us to consider social influences as well as about individual attitudes and beliefs. In the TTM frame, interventions designed for this population need to include contemplators and pre-contemplators and should focus on modification of cognition, affect and behaviors. Our research also shows that several of the underlying assumptions of TTM are at odds with the framework within which sex often occurs for this population and condom use decisions are highly influenced by the social context. In light of the results, the intervention placed condom use into a health promotion context. It combines group activities and one-on-one interaction. Group activities can impact shared values and beliefs and, thus, the intervention builds social support for behavior changes while addressing individual capacity

Baseline design of the filters for the LAD detector on board LOFT

The Large Observatory for X-ray Timing (LOFT) was one of the M3 missions selected for the phase A study in the ESA's Cosmic Vision program. LOFT is designed to perform high-time-resolution X-ray observations of black holes and neutron stars. The main instrument on the LOFT payload is the Large Area Detector (LAD), a collimated experiment with a nominal effective area of ~10 m 2 @ 8 keV, and a spectral resolution of ~240 eV in the energy band 2-30 keV. These performances are achieved covering a large collecting area with more than 2000 large-area Silicon Drift Detectors (SDDs) each one coupled to a collimator based on lead-glass micro-channel plates. In order to reduce the thermal load onto the detectors, which are open to Sky, and to protect them from out of band radiation, optical-thermal filter will be mounted in front of the SDDs. Different options have been considered for the LAD filters for best compromise between high quantum efficiency and high mechanical robustness. We present the baseline design of the optical-thermal filters, show the nominal performances, and present preliminary test results performed during the phase A study.Comment: Proc. SPIE 9144, Space Telescopes and Instrumentation 2014: Ultraviolet to Gamma Ray, 91446

The effect of water immersion on perception of the oculogravic illusion in normal and labyrinthine-defective subjects

Perception of oculogravic illusion in normal and labyrinthine-defective subjects related to water immersio