Predicing Ecological Effects of Watershed-Wide Rain Garden Implementation Using a Low-Cost Methodology

Stormwater control measures (SCMs) have been employed to mitigate peak flows and pollutants ssociated with watershed urbanization. Downstream ecological effects caused by the implementation of SCMs are largely unknown, especially at the watershed scale. Knowledge of these effects could help with setting goals for and targeting locations of local restoration efforts. Unfortunately, studies such as these typically require a high level of time and effort for the investigating party, of which resources are often limited. This study proposes a low-cost investigation method for the prediction of ecological effects on the watershed scale with the implementation of rain garden systems by using publicly available data and software. For demonstration purposes, a typical urban watershed was modeled using Storm Water Management Model (SWMM) 5.0. Forty-five models were developed in which the percent impervious area was varied 3 to 80%, and the fraction of rain gardens implemented with respect to the number of structures was varied from to 100%. The river chub fish (Nocomis micropogon) and its congeners (Nocomis spp.) were chosen as ecological indicators, as they are considered to be keystone species through interspecific nesting association. Depth and velocity criteria for successful nest building locations of the river chub were determined; these criteria can then be applied to many other watersheds. In this study, both base flow conditions and a typical summer storm event (1.3 cm, 6 h duration) were evaluated. During the simulated storm, nest-building locations were not affected in the 3 and 5% impervious cover models. Nest destruction was found to occur in approximately 54% of the original nest building sites for the 9% and 10% impervious areas. Nearly all of the nest-building locations were uninhabitable for impervious areas 20% and greater. Rain garden implementation significantly improved river chub habitat in the simulation, with greatest marginal benefit at lower levels of implementation

The \u3cem\u3emir-51\u3c/em\u3e Family of MicroRNAs Functions in Diverse Regulatory Pathways in \u3cem\u3eCaenorhbditis elegans\u3c/em\u3e

The mir-51 family of microRNAs (miRNAs) in C. elegans are part of the deeply conserved miR-99/100 family. While loss of all six family members (mir-51-56) in C. elegans results in embryonic lethality, loss of individual mir-51 family members results in a suppression of retarded developmental timing defects associated with the loss of alg-1. The mechanism of this suppression of developmental timing defects is unknown. To address this, we characterized the function of the mir-51 family in the developmental timing pathway. We performed genetic analysis and determined that mir-51 family members regulate the developmental timing pathway in the L2 stage upstream of hbl-1. Loss of the mir-51 family member, mir-52, suppressed retarded developmental timing defects associated with the loss of let-7 family members and lin-46. Enhancement of precocious defects was observed for mutations in lin-14, hbl-1, and mir-48(ve33), but not later acting developmental timing genes. Interestingly, mir-51 family members showed genetic interactions with additional miRNA-regulated pathways, which are regulated by the let-7 and mir-35 family miRNAs, lsy-6, miR-240/786, and miR-1. Loss of mir-52 likely does not suppress miRNA-regulated pathways through an increase in miRNA biogenesis or miRNA activity. We found no increase in the levels of four mature miRNAs, let-7, miR-58, miR-62 or miR-244, in mir-52 or mir-52/53/54/55/56 mutant worms. In addition, we observed no increase in the activity of ectopic lsy-6 in the repression of a downstream target in uterine cells in worms that lack mir-52. We propose that the mir-51 family functions broadly through the regulation of multiple targets, which have not yet been identified, in diverse regulatory pathways in C. elegans

The Consequences of Caregiving: Does Employment Make a Difference?

While a number of studies have examined the consequences of caregiving among employed women, surprisingly little research has explicitly compared how consequences differ between employed and not employed women. Moreover, very little research in this area has distinguished between part-time and full- time employment. This paper examines these issues drawing on the 1996 General Social Survey of Canada. The sample for this study consists of women aged 25 to 64 who reported providing care to one or more people aged 65+ because of a long-term physical disability (n=426). Three employment status groups (full-time, part-time and not employed) are compared on positive consequences, burden, guilt, job adjustment, postponed opportunities, and social and economic consequences. Results reveal significant differences between the three employment categories indicating that employment, both full and part-time, is associated with higher burden, guilt and social and economic consequences.caregiving; employment status; GSS

Loss of Individual MicroRNAs Causes Mutant Phenotypes in Sensitized Genetic Backgrounds in \u3cem\u3eC. elegans\u3c/em\u3e

MicroRNAs (miRNAs) are small, noncoding RNAs that regulate the translation and/or stability of their mRNA targets. Previous work showed that for most miRNA genes of C. elegans, single-gene knockouts did not result in detectable mutant phenotypes. This may be due, in part, to functional redundancy between miRNAs. However, in most cases, worms carrying deletions of all members of a miRNA family do not display strong mutant phenotypes. They may function together with unrelated miRNAs or with non-miRNA genes in regulatory networks, possibly to ensure the robustness of developmental mechanisms. To test this, we examined worms lacking individual miRNAs in genetically sensitized backgrounds. These include genetic backgrounds with reduced processing and activity of all miRNAs or with reduced activity of a wide array of regulatory pathways. With these two approaches, we identified mutant phenotypes for 25 out of 31 miRNAs included in this analysis. Our findings describe biological roles for individual miRNAs and suggest that the use of sensitized genetic backgrounds provides an efficient approach for miRNA functional analysis

Salvage Support and Operational Commanders: What They Need But May Not Get

Changes in both technology and the world political situation have increased the importance of salvage as a consideration at the operational level of maritime warfare. Its importance was easy to underemphasize when planning for the fast-moving, global, open-ocean conflict that was envisioned until the demise of the Soviet Union