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Additional file 4: of Infection of Anopheles aquasalis from symptomatic and asymptomatic Plasmodium vivax infections in Manaus, western Brazilian Amazon
Table S4. Plasmids dilutions containing the sequence of the respective PCR product were used both as assay standards and to determine the limit of detection (LoD) of each assay. Generation of the plasmids is described in [35, 38]. (DOC 29 kb
Additional file 2: of Infection of Anopheles aquasalis from symptomatic and asymptomatic Plasmodium vivax infections in Manaus, western Brazilian Amazon
Table S2. PCR cycling conditions used to detect parasites and gametocytes of Plasmodium vivax parasites. (DOC 32 kb
Additional file 3: of Infection of Anopheles aquasalis from symptomatic and asymptomatic Plasmodium vivax infections in Manaus, western Brazilian Amazon
Table S3. Data from each membrane feeding assay of Anopheles aquasalis performed with samples from symptomatic and asymptomatic individuals of Plasmodium vivax. (DOC 151 kb
Additional file 1: of Infection of Anopheles aquasalis from symptomatic and asymptomatic Plasmodium vivax infections in Manaus, western Brazilian Amazon
Table S1. PCR setup of Plasmodium-specific qPCR (QMAL) and P. vivax specific qPCR based on detection of 18S rRNA genes and RT-qPCR detecting pvs25 transcripts. For sequences of primers and probes see [35, 38]. (DOC 31 kb