Correction: Baseline Serum Osteopontin Levels Predict the Clinical Effectiveness of Tocilizumab but Not Infliximab in Biologic-Naïve Patients with Rheumatoid Arthritis: A Single-Center Prospective Study at 1 Year (the Keio First-Bio Cohort Study)

<p>Correction: Baseline Serum Osteopontin Levels Predict the Clinical Effectiveness of Tocilizumab but Not Infliximab in Biologic-Naïve Patients with Rheumatoid Arthritis: A Single-Center Prospective Study at 1 Year (the Keio First-Bio Cohort Study)</p

Categorical changes in clinical indices.

<p>(A) Clinical Disease Activity Index (CDAI), (B) Simplified Disease Activity Index (SDAI), and (C) disease activity score with 28 joint counts, erythrocyte sedimentation rate (DAS28-ESR), over 1-year treatment of infliximab (IFX) or tocilizumab (TCZ). H, high disease activity; M, moderate disease activity; L, low disease activity; R, remission.</p

Predictive ability of osteopontin for clinical remission in patients with RA who received tocilizumab (TCZ) or infliximab (IFX).

<p>(A) Logistic regression analysis showing significant association of increasing baseline osteopontin (OPN) levels with decreasing predicted probability of achieving Clinical Disease Activity Index (CDAI) remission at 1 year in the TCZ (tocilizumab) group. (B) ROC curve showing a cut-off baseline OPN level of 17.3 ng/mL, discriminating between CDAI remission and non-remission at 1 year in the TCZ group, with a sensitivity of 66% and a specificity of 80%. (C) Logistic regression analysis showing no significant association of baseline OPN levels with predicted probability of achieving CDAI remission at 1 year in the IFX (infliximab) group. Categorical changes in clinical indices such as (D) CDAI, (E) Simplified Disease Activity Index (SDAI), and (F) disease activity score with 28 joint counts, erythrocyte sedimentation rate (DAS28-ESR) over 1-year treatment of TCZ or IFX, stratified by low or high baseline OPN levels (cut-off: 17.3 ng/mL). H, high disease activity; M, moderate disease activity; L, low disease activity; R, remission.</p

Additional file 1: Figure S1. of A new bioassay for measuring the strength of IL-6/STAT3 signal inhibition by tocilizumab in patients with rheumatoid arthritis

Transition in CDAI for each group of patients administered TCZ at different intervals. Transition in CDAI for the 3-week group (A), 4-week group (B) and 5-week group (C). TCZ tocilizumab, CDAI clinical disease activity index. (TIF 1404 kb

Additional file 2: Figure S1. of Identification of definitive serum biomarkers associated with disease activity in primary Sjögren’s syndrome

Functional annotation of differentially expressed proteins in pSS patient sera. Nodes indicate molecular concepts or set of biologically related genes. Name of each node is indicated in black text on the node. The node size represents the proportion of differentially expressed gene symbols in the concepts (e.g., the “chemokine signaling pathway” and “extracellular region” concepts contain 14 and 58 genes, respectively). Length of lines between nodes represents degree of overlap between symbols. Colored lines indicate strength of functional relationship from strong to weak, as follows: red, yellow, green and gray. Green nodes indicate immune response-related molecular concepts, and red nodes indicate platelet-related molecular concepts. (TIF 9752 kb

Additional file 3: Figure S2. of Identification of definitive serum biomarkers associated with disease activity in primary Sjögren’s syndrome

Serum levels of five proteins in pSS, sSS, sicca syndrome and HCs. The five proteins were CXCL13, TNF-R2, CD48, BAFF and PD-L2. Primary SS (pSS), n = 58; secondary SS (sSS), n = 6; other sicca syndrome, n = 13; healthy controls (HCs), n = 38. Differences in quantitative variables were analyzed by the Mann-Whitney U test when comparing two groups and by the Kruskal-Wallis test when comparing multiple groups. *P value <0.05, which was considered significant. (TIF 33972 kb

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Background<p>Lymphoproliferative disorder (LPD), including malignant lymphoma, is a relatively rare but life-threatening complication in RA patients under methotrexate (MTX) therapy. Spontaneous regression of LPD after MTX withdrawal is regarded as a distinct characteristic in part of such LPDs.</p>Objective<p>The present study aimed to investigate the immunological difference in regressive LPD and persistent LPD.</p>Methods<p>We studied RA patients who developed LPD during MTX administration (n = 35) and clinically matched controls (n = 35). The time of MTX cessation was defined as week 0, and LPD patients were divided into two groups according to LPD status at week 12: regressive group (n = 22) and persistent group (n = 13). Flow cytometric analysis of whole blood samples and serum cytokine assays were conducted for LPD (n = 10) and control patients (n = 10) at weeks 0, 4, and 12.</p>Results<p>There was a significant decrease in peripheral lymphocytes and the proportion of T helper 1 cells (Th1 cells), effector memory CD8+ T cells (EMCD8+ T) and Epstein–Barr virus (EBV)-specific CD8+ T cells at the time of LPD diagnosis, and a significant increase after MTX cessation was observed in the regressive group but not in the persistent group. The expansion of Th1 cells and EMCD8+ T cells significantly correlated with an increase in serum interferon (IFN)-γ concentration.</p>Conclusion<p>Changes in Th1 cells, EMCD8+ T cells and EBV-specific CD8+ T cells, which coincided with an increase in IFN-γ, were significantly different between regressive LPD and persistent LPD after MTX cessation.</p