On the pulse of change: the new beat of special education in higher education

The roles and responsibilities of special educators in both special and regular school settings are changing rapidly. More than two decades ago the move towards more inclusive practices disrupted the traditional, niche separatism of special educators to the extent that they are now an integral part of the regular school teaching staff. Today the broad agenda to facilitate access and participation for all students in education, not just students with disabilities, influences the roles and responsibilities of special educators. This article briefly identifies some of the generic pulses that are moving the special education profession from a focus on low incidence disabilities towards a more comprehensive approach to inclusion, school responsiveness and individualised learning pathways. From the foundation of inclusive practice, this paper will describe how a qualitative study was used to understand the changing roles and responsibilities of special educators. A case study analysis of 17 schools formed the basis of the investigation. Principals, lead teachers in special education and special education teachers were asked to identify trends and priorities in special education and also to identify rewards and challenges in their jobs. Further cross referencing with teacher and special educator standards, a focus group, a stakeholder group and research in the field increased the opportunity for academics and special educators to critically reflect on the emerging demands placed on special educators and the attributes that are needed to be professionally effective

Characterization of the Arsenate Respiratory Reductase from Shewanella sp. Strain ANA-3

Microbial arsenate respiration contributes to the mobilization of arsenic from the solid to the soluble phase in various locales worldwide. To begin to predict the extent to which As(V) respiration impacts arsenic geochemical cycling, we characterized the expression and activity of the Shewanella sp. strain ANA-3 arsenate respiratory reductase (ARR), the key enzyme involved in this metabolism. ARR is expressed at the beginning of the exponential phase and persists throughout the stationary phase, at which point it is released from the cell. In intact cells, the enzyme localizes to the periplasm. To purify ARR, a heterologous expression system was developed in Escherichia coli. ARR requires anaerobic conditions and molybdenum for activity. ARR is a heterodimer of ~131 kDa, composed of one ArrA subunit (~95 kDa) and one ArrB subunit (~27 kDa). For ARR to be functional, the two subunits must be expressed together. Elemental analysis of pure protein indicates that one Mo atom, four S atoms associated with a bis-molybdopterin guanine dinucleotide cofactor, and four to five [4Fe-4S] are present per ARR. ARR has an apparent melting temperature of 41°C, a Km of 5 µM, and a Vmax of 11,111 µmol of As(V) reduced min–1 mg of protein–1 and shows no activity in the presence of alternative electron acceptors such as antimonite, nitrate, selenate, and sulfate. The development of a heterologous overexpression system for ARR will facilitate future structural and/or functional studies of this protein family

Principal’s stress: tensions between the requirements of the disability discrimination legislation and the governance of inclusion in schools

This paper examines qualitative perspectives that principals in schools have of the disability discrimination legislation. Perspectives from in-depth interviews with six school principals and two focus groups of twenty-eight principals describe attitudes toward the disability discrimination legislation, particularly as these attitudes relate to the governance of inclusion. The study found that a reduced level of knowledge of the disability discrimination legislation and vague, ineffectual inclusive education policies contributed to leadership problems in the governance of inclusion. A combination of such factors increased stressful, reactive decision-making responses from principals particularly when complex school situations related to students with disabilities and challenging behaviours. The study proposed that collaborative decision-making to create shared understandings about disability issues was more effective in creating inclusive school cultures than the imposed requirements of the disability discrimination legislation

Exhaustive mutagenesis of six secondary active-site residues in Escherichia coli chorismate mutase shows the importance of hydrophobic side chains and a helix N-capping position for stability and catalysis

Secondary active-site residues in enzymes, including hydrophobic amino acids, may contribute to catalysis through critical interactions that position the reacting molecule, organize hydrogen-bonding residues, and define the electrostatic environment of the active site. To ascertain the tolerance of an important model enzyme to mutation of active-site residues that do not directly hydrogen bond with the reacting molecule, all 19 possible amino acid substitutions were investigated in six positions of the engineered chorismate mutase domain of the Escherichia coli chorismate mutase-prephenate dehydratase. The six secondary active-site residues were selected to clarify results of a previous test of computational enzyme design procedures. Five of the positions encode hydrophobic side chains in the wild-type enzyme, and one forms a helix N-capping interaction as well as a salt bridge with a catalytically essential residue. Each mutant was evaluated for its ability to complement an auxotrophic chorismate mutase deletion strain. Kinetic parameters and thermal stabilities were measured for variants with in vivo activity. Altogether, we find that the enzyme tolerated 34% of the 114 possible substitutions, with a few mutations leading to increases in the catalytic efficiency of the enzyme. The results show the importance of secondary amino acid residues in determining enzymatic activity, and they point to strengths and weaknesses in current computational enzyme design procedures

Computationally designed variants of Escherichia coli chorismate mutase show altered catalytic activity

Computational protein design methods were used to predict five variants of monofunctional Escherichia coli chorismate mutase expected to maintain catalytic activity. The variants were tested experimentally and three active site mutants exhibited catalytic activity similar to or greater than the wild-type enzyme. One mutant, Ala32Ser, showed increased catalytic efficiency

Designated Driver-less Cars? Why Current Georgia Law Supports Liability for Intoxicated Drivers of Autonomous Vehicles

In recent years, autonomous vehicles (AVs), once the province of science fiction, have become an increasingly common sight on American roads. This new technology raises a host of novel legal questions, one of which is how driving under the influence (DUI) law should apply to AVs. Georgia has authorized the use of AVs, and some lawmakers who voted in favor of that authorization did so because, at least in part, they felt AVs could reduce the number of DUIs in Georgia. This Note argues, however, that Georgia DUI law as written and interpreted by the Georgia courts would apply to hold the operator of any currently existing AV criminally responsible for DUI if they operated the AV while under the influence of drugs or alcohol. Therefore, if authorizing AV use intended to alter the scheme of Georgia DUI law for these vehicles, then amendments to Georgia’s DUI statutes or a new statutory DUI scheme specifically applicable to AVs must be adopted to reflect that intent