Time course of transcription factor induction after stimulation with TPA and ionomycin

<p><b>Copyright information:</b></p><p>Taken from "Cytokine profiles of cord and adult blood leukocytes: differences in expression are due to differences in expression and activation of transcription factors"</p><p>http://www.biomedcentral.com/1471-2172/8/18</p><p>BMC Immunology 2007;8():18-18.</p><p>Published online 31 Aug 2007</p><p>PMCID:PMC2018703.</p><p></p> ABL and CBL were harvested at different time points by lysing cells. mRNA levels were determined for T-bet (A), GATA-3 (B), and c-maf (C). Data points are mean ± standard error for 6 donors. *< 0.05 ABL vs. CBL

Kinetics of cytokine induction after stimulation with TPA and ionomycin

<p><b>Copyright information:</b></p><p>Taken from "Cytokine profiles of cord and adult blood leukocytes: differences in expression are due to differences in expression and activation of transcription factors"</p><p>http://www.biomedcentral.com/1471-2172/8/18</p><p>BMC Immunology 2007;8():18-18.</p><p>Published online 31 Aug 2007</p><p>PMCID:PMC2018703.</p><p></p> ABL and CBL were harvested at different time points by lysing cells. mRNA levels for IFNγ (A), IL-4 (B), and IL-3 (C) were determined as in Figure 1. Data points are the mean ± standard error for 6 donors. *< 0.05, **< 0.01 ABL vs. CBL

Induction of transcription factors in ABL and CBL after stimulation for 4 hrs

<p><b>Copyright information:</b></p><p>Taken from "Cytokine profiles of cord and adult blood leukocytes: differences in expression are due to differences in expression and activation of transcription factors"</p><p>http://www.biomedcentral.com/1471-2172/8/18</p><p>BMC Immunology 2007;8():18-18.</p><p>Published online 31 Aug 2007</p><p>PMCID:PMC2018703.</p><p></p> mRNA levels of T-bet (A), GATA-3 (B), and c-maf (C) were determined using real-time RT-PCR. Data are expressed as arbitrary units normalized to β-actin to correct for RNA quantity and integrity. Columns are the mean ± standard for 3 donors. The inset shows the correlation between expression of the respective transcription factor with IFNγ and IL-4. Dotted line indicates basal transcript levels. Iono – Ionomycin