4 research outputs found
Data_Sheet_3_The differential effect of two cereal foods on gut environment: a randomized, controlled, double-blind, parallel-group study.xlsx
Background and aimsCereal-based foods such as fruit granola (FG) and corn flakes (CF) form part of a fiber-rich diet. Dietary fiber has a good effect on human health. However, changes in gut microbiota and intestinal immunity have not been investigated. We conducted a randomized, double-blind, placebo-controlled trial to investigate the effects of FG and CF intake on gut microbiota, metabolome, and the immune system.MethodsSubjects continuously consume CF or FG for 4 weeks. Stool samples, and questionnaires on defecation were collected before, 2 weeks after, and 4 weeks after intake. Gut microbiota was analyzed using 16S rRNA gene amplicon sequencing. Fecal metabolomes were analyzed using GC/MS and CE-TOF/MS. Fecal IgA was analyzed using ELISA.ResultsThe defecation frequency after cereal based food intake was improved. The different cereal-based foods had different effects on gut microbiome. The increase in intestinal IgA levels was positively correlated with the relative abundance of Dialister and the Lachnospiraceae ND3007 group in CF and FG group, respectively. SCFAs showed a positive correlation with Prevotella 9 in the FG group.ConclusionThis study showed that the supplement in dietary fiber contained in CF and FG improves bowel movements. CF and FG each had different effects on gut microbes, metabolites and different relationships between fecal IgA or SCFAs and gut microbiota.</p
Data_Sheet_1_The differential effect of two cereal foods on gut environment: a randomized, controlled, double-blind, parallel-group study.xlsx
Background and aimsCereal-based foods such as fruit granola (FG) and corn flakes (CF) form part of a fiber-rich diet. Dietary fiber has a good effect on human health. However, changes in gut microbiota and intestinal immunity have not been investigated. We conducted a randomized, double-blind, placebo-controlled trial to investigate the effects of FG and CF intake on gut microbiota, metabolome, and the immune system.MethodsSubjects continuously consume CF or FG for 4 weeks. Stool samples, and questionnaires on defecation were collected before, 2 weeks after, and 4 weeks after intake. Gut microbiota was analyzed using 16S rRNA gene amplicon sequencing. Fecal metabolomes were analyzed using GC/MS and CE-TOF/MS. Fecal IgA was analyzed using ELISA.ResultsThe defecation frequency after cereal based food intake was improved. The different cereal-based foods had different effects on gut microbiome. The increase in intestinal IgA levels was positively correlated with the relative abundance of Dialister and the Lachnospiraceae ND3007 group in CF and FG group, respectively. SCFAs showed a positive correlation with Prevotella 9 in the FG group.ConclusionThis study showed that the supplement in dietary fiber contained in CF and FG improves bowel movements. CF and FG each had different effects on gut microbes, metabolites and different relationships between fecal IgA or SCFAs and gut microbiota.</p
Data_Sheet_2_The differential effect of two cereal foods on gut environment: a randomized, controlled, double-blind, parallel-group study.docx
Background and aimsCereal-based foods such as fruit granola (FG) and corn flakes (CF) form part of a fiber-rich diet. Dietary fiber has a good effect on human health. However, changes in gut microbiota and intestinal immunity have not been investigated. We conducted a randomized, double-blind, placebo-controlled trial to investigate the effects of FG and CF intake on gut microbiota, metabolome, and the immune system.MethodsSubjects continuously consume CF or FG for 4 weeks. Stool samples, and questionnaires on defecation were collected before, 2 weeks after, and 4 weeks after intake. Gut microbiota was analyzed using 16S rRNA gene amplicon sequencing. Fecal metabolomes were analyzed using GC/MS and CE-TOF/MS. Fecal IgA was analyzed using ELISA.ResultsThe defecation frequency after cereal based food intake was improved. The different cereal-based foods had different effects on gut microbiome. The increase in intestinal IgA levels was positively correlated with the relative abundance of Dialister and the Lachnospiraceae ND3007 group in CF and FG group, respectively. SCFAs showed a positive correlation with Prevotella 9 in the FG group.ConclusionThis study showed that the supplement in dietary fiber contained in CF and FG improves bowel movements. CF and FG each had different effects on gut microbes, metabolites and different relationships between fecal IgA or SCFAs and gut microbiota.</p
Potato Chip Intake Increases Ascorbic Acid Levels and Decreases Reactive Oxygen Species in SMP30/GNL Knockout Mouse Tissues
Potato
chips (PC) contain abundant amounts of the free radical
scavenger ascorbic acid (AA) due to the rapid dehydration of potato
tubers (Solanum tuberosum) that occurs
during frying. To evaluate the antioxidant activity of PC, this study
examined reactive oxygen species (ROS) levels in tissues from SMP30/GNL
knockout (KO) mice that cannot synthesize AA and determined AA and
ROS levels after the animals were fed 20 and 10% PC diets for 7 weeks.
Compared with AA-sufficient mice, AA-depleted SMP30/GNL KO mice showed
high ROS levels in tissues. SMP30/GNL KO mice fed a PC diet showed
high AA and low ROS levels in the brain, heart, lung, testis, soleus
muscle, plantaris muscle, stomach, small intestine, large intestine,
eyeball, and epididymal fat compared with AA-depleted mice. The data
suggest that PC intake increases AA levels and enhances ROS scavenging
activity in tissues of SMP30/GNL KO mice, which are a promising model
for evaluating the antioxidant activity of foods