Additional file 3: Figure S3. of Molecular and behavioral profiling of Dbx1-derived neurons in the arcuate, lateral and ventromedial hypothalamic nuclei

Increased c-Fos in Arc, LH, and VMH after innate behaviors. (A-D.i) Images of the c-Fos expression in the Arc (A-D), LH (E-H), and VMH (I-L) after mice were exposed to mating (A, E, and I), aggression (B, F, and J), fasting (C, G, and K), or predator (D, H, and L) behavioral paradigms. The number of cells expressing c-Fos in the Arc, LH, and VMH are increased after exposure to the four behavioral paradigms. The scale bar represents 500 μm in the LH and VMH (A-D.i, I-L.i) and 250 μm in the Arc (E-H.i). (TIF 172032 kb

Additional file 2: Figure S2. of Molecular and behavioral profiling of Dbx1-derived neurons in the arcuate, lateral and ventromedial hypothalamic nuclei

Dbx1-derived cells in nuclei as defined by specific markers. (A-D) Schematic of medial (top) to lateral (bottom) sagittal views of the embryonic forebrain. (A.i-D.i) As shown by YFP expression in E13.5 Dbx1 Cre ;Rosa26YFP embryos, Dbx1-derived cells are are found in primordial hypothalamic nuclei including in the paraventricular, arcuate, ventral medial and lateral progenitor domains. (A.ii-D.iii) As shown by ISH of serial sections, specific markers of the paraventriclar (Sim1, A.ii; Fezf1, A.iii), arcuate (Pomc, B.ii; Bsx, B.iii), ventral medial (Fezf1, C.ii; Nr5a1, C.iii) and lateral hypothalamic (Pmch, D.ii; Lhx9, D.iii) nuclei overlap with expression of YFP. The scale bar represents 500 μm. (TIF 157696 kb