Additional file 1: of Inhibition of integrin αVβ6 changes fibril thickness of stromal collagen in experimental carcinomas

FACS analysis showing that both KAT-4 and Capan-2 tumor cells expressed αvβ6 integrin on their cell surfaces (black peaks). IgG is negative control (grey peaks). (TIF 144 kb

Additional file 2: of Inhibition of integrin αVβ6 changes fibril thickness of stromal collagen in experimental carcinomas

TGF-β activation and inhibition in fibroblast and Capan-2 co-cultured with MLEC. Data was analyzed with Student’s t-test. Error bars are standard deviations. A) TGF-β activation inhibition with Cilengitide (0 to 10 μM). B) TGF-β activation inhibition with cRGD peptide (0 to 10 μM). C) Effects on TGF-β activation by co-culturing KAT-4 cells (Kat4, K) or Capan-2 (Capan2, C) together with MLEC reporter cells (M) on plastic dishes coated with human plasma fibronectin (HFN) or human plasma vitronectin (Vn). (TIF 8227 kb

Additional file 5: of Inhibition of integrin αVβ6 changes fibril thickness of stromal collagen in experimental carcinomas

Effects of the tyrosine kinase inhibitor imatinib (STI571) on TGF β-induced PAI-1 expression in mink lung epithelial cells (MLEC) was recorded as detailed in Material and Methods. The signal recorded after stimulation with 10 nM TGF-β was normalized to 1 (n = 6). The effect on TGF-β-elicited signaling by 10 μM STI571 is shown (p < 0.0001, n = 3). Error bars +/− SD. All reagents were added simultaneously to the MLEC cultures at the start of the incubations. (TIF 393 kb

Additional file 3: of Inhibition of integrin ιVβ6 changes fibril thickness of stromal collagen in experimental carcinomas

Differential gene expression in KAT-4 vs. Capan-2 single-cell cultures. Transcripts with log2 fold change > 4 are shown and annotated using GO terms or experimental evidence reported in Uniprot. Positive fold change means higher transcript levels in Capan-2, negative fold change means higher transcript levels in KAT-4. (PDF 44 kb