170 research outputs found
The influence of endosome-disruptive peptides on gene transfer using synthetic virus-like gene transfer systems
DNA-binding transferrin conjugates as functional gene-delivery agents: synthesis by linkage of polylysine or ethidium homodimer to the transferrin carbohydrate moiety
We have previously demonstrated that transferrin-polycation conjugates are efficient carrier molecules
for the introduction of genes into eucariotic cells. We describe here a more specific method for conjugation
of transferrin with DNA-binding compounds involving attachment at the transferrin carbohydrate
moiety. We used the polycation poly(L-lysine) or the DNA intercalator, ethidium homodimer as DNAbinding
domains. Successful transferrin-receptor-mediatedd elivery and expression of the Photinus
pyralis luciferase gene in K562 cells has been shown with these new transferrin conjugates. The activity
of the transferrin-ethidium homodimer (TfEtD) conjugates is low relative to transferrin-polylysine
conjugates; probably because of incomplete condensation of the DNA. However, DNA delivery with
TfEtD is drastically improved when ternary complexes of the DNA with TfEtD and the DNA condensing
agent polylysine are prepared. The gene delivery with the carbohydrate-linked transferrin-polylysine
conjugates is equal or superior to described conjugates containing disulfide linkage. The new ligation
method facilitates the synthesis of large quantities (>lo0 mg) of conjugates.
INTRODUCTION
Transferrin-polycation conjugates are efficient carriers
for the uptake of DNA into eucariotic cells (I). This gene
transfer technique, termed tramferrinfection, is based
on receptor-mediated endocytosis of DNA complexed with
polycation-transferrin conjugates (2,3). Our initial conjugate
synthesis (1) involved the modification of one to
two amino groups on the transferrin molecule with the
bifunctional reagent succinimidyl34 2-pyridy1dithio)propionate
(SPDP), followed by ligation to similarly modified
polycations (polylysine or protamine) through the formation
of disulfide bonds. Because there are more than
50 lysines on the large (about 80 kDa) transferrin protein,
the actual site (or sites) of ligation to the polycation is
unknown with this method.
In this paper we describe the synthesis of new transferrin
conjugates that are ligated with DNA-binding
compounds in a specific manner through modification of
the transferrin carbohydrate moiety. The conjugates thus
obtained are free of any groups derived from chemical
linking agents, since the connecting atoms are already
present within the starting compounds. The carbohydrate
group acts as anatural spacer that puts a 32-atom distance
between the transferrin and the DNA binding moiety. This
spacer effect may be important for appropriate presentation
of the ligand to its receptor. As a DNA-binding
compound, the polycation polylysine was used, similar to
the use described in ref 1 or to the asialo-orosomucoid
conjugates prepared by Wu and Wu (4). We have also
prepared a novel type of transferrin conjugate that contains
the DNA intercalator ethidium homodimer (5) as the DNAbinding
group and demonstrate successful receptormediated
gene delivery with these conjugates.
EXPERIMENTAL PROCEDURES
Human transferrin (iron-free), conalbumin (iron-free),
and poly(L-lysine) were obtained from Sigma. Liquid chro-
Abbreviations used: FITC, fluorescein ieothiocyenate; TfEtD,
traneferrin-ethidium homodimer conjugate; TfpL, traneferrinpolytL-
lysine) conjugate; HEPES, 4-(2-hydroxyethyl)-l-piperazineethanesulfonic
acid
Coupling of adenovirus to transferrin-polylysine/DNA complexes greatly enhances receptor-mediated gene delivery and expression of transfected genes.
We are developing efficient methods for gene transfer into tissue culture cells. We have previously shown that coupling of a chimeric adenovirus with polylysine allowed the construction of an adenovirus-polylysine-reporter-gene complex that transferred the transporter gene with great efficiency into HeLa cells. We have now explored simpler, biochemical means for coupling adenovirus to DNA/polylysine complexes and show that such complexes yield virtually 100% transfection in tissue culture cell lines. In these methods adenovirus is coupled to polylysine, either enzymatically through the action of transglutaminase or biochemically by biotinylating adenovirus and streptavidinylating the polylysine moiety. Combination complexes containing DNA, adenovirus-polylysine, and transferrin-polylysine have the capacity to transfer the reporter gene into adenovirus-receptor- and/or transferrin-receptor-rich cells
A synthetic peptide library for benchmarking crosslinking-mass spectrometry search engines for proteins and protein complexes
Crosslinking-mass spectrometry (XL-MS) serves to identify interaction sites between proteins. Numerous search engines for crosslink identification exist, but lack of ground truth samples containing known crosslinks has precluded their systematic validation. Here we report on XL-MS data arising from measuring synthetic peptide libraries that provide the unique benefit of knowing which identified crosslinks are true and which are false. The data are analysed with the most frequently used search engines and the results filtered to an estimated false discovery rate of 5%. We find that the actual false crosslink identification rates range from 2.4 to 32%, depending on the analysis strategy employed. Furthermore, the use of MS-cleavable crosslinkers does not reduce the false discovery rate compared to non-cleavable crosslinkers. We anticipate that the datasets acquired during this research will further drive optimisation and development of XL-MS search engines, thereby advancing our understanding of vital biological interactions
Carbohydrate receptor-mediated gene transfer to human T leukaemic cells
The mucin-type carbohydrate Tn cryptantigen (GalNAcα1-O-Ser/Thr, where GalNAc is N-acetyl-D-galactosamine) is expressed in many carcinomas, in haemopoietic disorders including the Tn syndrome, and on human immunodeficiency virus (HIV) coat glycoproteins, but is not expressed on normal, differentiated cells because of the expression of a Tn-processing galactosyltransferase. Using Jurkat T leukaemic cells which express high levels of Tn antigen due to deficient Tn galactosylation, we have established the Tn antigen-mediated gene transfer and demonstrate the considerable efficiency of this approach. We used poly(L-lysine) conjugates of the monoclonal antibody 1E3 directed against the Tn antigen to deliver the luciferase and β-galactosidase reporter genes to Jurkat cells by receptor-mediated endocytosis. Addition of unconjugated 1E3 reduced transfection efficiency in a concentration-dependent manner and incubation with free GalNAc abolished DNA transfer completely, indicating that gene delivery is indeed mediated by the Tn antigen. Pre-treatment of Jurkat cells with Vibrio cholerae sialidase, which uncovers additional Tn antigens, resulted in an improvement of gene transfection. Both human and chicken adenovirus particles attached to the DNA/polylysine complex strongly augmented transgene expression. When the β-galactosidase (lacZ) gene was delivered to Jurkat cells by Tn-mediated endocytosis, up to 60% of the cells were positive in the cytochemical stain using 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal) as a chromogenic substrate. The efficiency of the transferrin receptor-mediated DNA uptake into Jurkat cells was comparatively low, although these cells were shown to express considerable amounts of transferrin receptor. We show here that a mucin-type carbohydrate antigen mediates highly efficient DNA uptake by endocytosis into Jurkat T cells. This method represents a 50-fold improvement of Jurkat cell transfection efficiency over other physical gene transfer techniques. Specific gene delivery to primary cancer cells exhibiting Tn epitopes may especially be desirable in immunotherapy protocol
Coupling of adenovirus to transferrin-polylysine/DNA complexes greatly enhances receptor-mediated gene delivery and expression of transfected genes.
We are developing efficient methods for gene transfer into tissue culture cells. We have previously shown that coupling of a chimeric adenovirus with polylysine allowed the construction of an adenovirus-polylysine-reporter-gene complex that transferred the transporter gene with great efficiency into HeLa cells. We have now explored simpler, biochemical means for coupling adenovirus to DNA/polylysine complexes and show that such complexes yield virtually 100% transfection in tissue culture cell lines. In these methods adenovirus is coupled to polylysine, either enzymatically through the action of transglutaminase or biochemically by biotinylating adenovirus and streptavidinylating the polylysine moiety. Combination complexes containing DNA, adenovirus-polylysine, and transferrin-polylysine have the capacity to transfer the reporter gene into adenovirus-receptor- and/or transferrin-receptor-rich cells
Transcriptome and proteome quantification of a tumor model provides novel insights into post‐transcriptional gene regulation
Reducing the haystack to find the needle: improved protein identification after fast elimination of non-interpretable peptide MS/MS spectra and noise reduction
Drosophila CPEB Orb2A Mediates Memory Independent of Its RNA-Binding Domain
SummaryLong-term memory and synaptic plasticity are thought to require the synthesis of new proteins at activated synapses. The CPEB family of RNA binding proteins, including Drosophila Orb2, has been implicated in this process. The precise mechanism by which these molecules regulate memory formation is however poorly understood. We used gene targeting and site-specific transgenesis to specifically modify the endogenous orb2 gene in order to investigate its role in long-term memory formation. We show that the Orb2A and Orb2B isoforms, while both essential, have distinct functions in memory formation. These two isoforms have common glutamine-rich and RNA-binding domains, yet Orb2A uniquely requires the former and Orb2B the latter. We further show that Orb2A induces Orb2 complexes in a manner dependent upon both its glutamine-rich region and neuronal activity. We propose that Orb2B acts as a conventional CPEB to regulate transport and/or translation of specific mRNAs, whereas Orb2A acts in an unconventional manner to form stable Orb2 complexes that are essential for memory to persist
Crosstalk between H2A variant-specific modifications impacts vital cell functions
Selection of C-terminal motifs participated in evolution of distinct histone H2A variants. Hybrid types of variants combining motifs from distinct H2A classes are extremely rare. This suggests that the proximity between the motif cases interferes with their function. We studied this question in flowering plants that evolved sporadically a hybrid H2A variant combining the SQ motif of H2A.X that participates in the DNA damage response with the KSPK motif of H2A.W that stabilizes heterochromatin. Our inventory of PTMs of H2A.W variants showed that in vivo the cell cycle-dependent kinase CDKA phosphorylates the KSPK motif of H2A. W but only in absence of an SQ motif. Phosphomimicry of KSPK prevented DNA damage response by the SQ motif of the hybrid H2A.W/X variant. In a synthetic yeast expressing the hybrid H2A.W/X variant, phosphorylation of KSPK prevented binding of the BRCT-domain protein Mdb1 to phosphorylated SQ and impaired response to DNA damage. Our findings illustrate that PTMs mediate interference between the function of H2A variant specific C-terminal motifs. Such interference could explain the mutual exclusion of motifs that led to evolution of H2A variants.Fil: Schmücker, Anna. Austrian Academy Of Sciences (oaw);Fil: Lei, Bingkun. Austrian Academy Of Sciences (oaw);Fil: Lorkovic, Zdravko J.. Ludwig Maximilians Universitat; AlemaniaFil: Capella, Matias. Ludwig Maximilians Universitat; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Braun, Sigurd. Ludwig Maximilians Universitat; AlemaniaFil: Bourguet, Pierre. Gregor Mendel Institute Of Molecular Plant Biology; Austria. Université Clermont Auvergne; FranciaFil: Mathieu, Olivier. Université Clermont Auvergne; FranciaFil: Mechtler, Karl. Gregor Mendel Institute Of Molecular Plant Biology; AustriaFil: Berger, Frédéric. Gregor Mendel Institute Of Molecular Plant Biology; Austri
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