Cytochrome c oxidase subunit 1 gene as a DNA barcode for discriminating Trypanosoma cruzi DTUs and closely related species

Submitted by Sandra Infurna ([email protected]) on 2018-02-10T19:03:03Z No. of bitstreams: 1 marina_rodrigues_etal_IOC_2017.pdf: 2213050 bytes, checksum: 3c437c2797655c7c7019affeee453f4c (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2018-02-10T19:12:31Z (GMT) No. of bitstreams: 1 marina_rodrigues_etal_IOC_2017.pdf: 2213050 bytes, checksum: 3c437c2797655c7c7019affeee453f4c (MD5)Made available in DSpace on 2018-02-10T19:12:31Z (GMT). No. of bitstreams: 1 marina_rodrigues_etal_IOC_2017.pdf: 2213050 bytes, checksum: 3c437c2797655c7c7019affeee453f4c (MD5) Previous issue date: 2017Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de tripanosomatídeos. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcântara Gomes. Departamento de Ecologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de tripanosomatídeos. Rio de Janeiro, RJ, Brasil.The DNA barcoding system using the cytochrome c oxidase subunit 1 mitochondrial gene (cox1 or COI) is highly efficient for discriminating vertebrate and invertebrate species. In the present study, we examined the suitability of cox1 as a marker for Trypanosoma cruzi identification from other closely related species. Additionally, we combined the sequences of cox1 and the nuclear gene glucose-6-phosphate isomerase (GPI) to evaluate the occurrence of mitochondrial introgression and the presence of hybrid genotypes

Exploring the environmental diversity of kinetoplastid flagellates in the high-throughput DNA sequencing era

International audienceThe class Kinetoplastea encompasses both free-living and parasitic species from a wide range of hosts. Several representatives of this group are responsible for severe human diseases and for economic losses in agriculture and livestock. While this group encompasses over 30 genera, most of the available information has been derived from the vertebrate pathogenic genera Leishmania and Trypanosoma. Recent studies of the previously neglected groups of Kinetoplastea indicated that the actual diversity is much higher than previously thought. This article discusses the known segment of kinetoplastid diversity and how gene-directed Sanger sequencing and next-generation sequencing methods can help to deepen our knowledge of these interesting protists