Synthesis of Silver Nanorods from Food Industrial Waste and Their Application in Improving the Keeping Quality of Milk

A novel method for the synthesis of silver nanorods is reported, in which industrial milk waste was utilized, that were then used to extend the stability of milk. During the synthesis, the size of the silver nanorods were affected by pH and temperature. Silver nanorods were formed at alkaline pH in room temperature, whereas nanoparticles were formed in lower pH at elevated temperature. The obtained nanostructures were characterized by UV–visible spectrophotometer, energy dispersive X-ray analysis (EDAX), and transmission electron microscope (TEM). These silver nanorods were used to control coliform and standard plate count (SPC) in milk. This was confirmed by an increase in 4 to 5 folds of methylene blue reduction time as compared to the control. The Hom inactivation model was proposed to express microbial inactivation in milk. The cytotoxic effect of silver nanorods shows that they have been nontoxic to humans even at higher concentration

Additional file 1: Figure S1. of Shape- and Size-Controlled Synthesis of Silver Nanoparticles Using Aloe vera Plant Extract and Their Antimicrobial Activity

Comparison among inhibitions by AgNP (by chemical method), AgNP with Aloe vera plant extract, only Aloe vera plant extract and only AgNO3 solution. 1. Staphylococcus aureus; 2. Bacillus cereus; 3. Micrococcus luteus; 4. Escherichia coli; 5. Klebsiella pneumoniae. The values were determined based on statistical standard deviation with the triplicate values. The calculated standard deviations were between Âą1 and Âą2. (DOCX 84 kb

Ultraviolet–Visible spectroscopy.

<p>(A) CMC@AgNPs at (a) 30s, (b) 60s, (c) 90s, (d) 120s, (e) 150s, (f) 180s, (g) 210s and (h) 240s. (B) SA@AgNPs at (a) 30s, (b) 60s, (c) 90s, (d) 120s, (e) 150s, (f) 180s, (g) 210s and (h) 240s. Arrow indicates direction of spectral changes.</p

Biopolymers Regulate Silver Nanoparticle under Microwave Irradiation for Effective Antibacterial and Antibiofilm Activities - Fig 7

<p><b>(A) Antibiofilm efficacy of</b> CMC@AgNPs <b>and (B)</b> SA@AgNPs <b>against both <i>E</i>. <i>coli</i> and <i>S</i>. <i>aureus</i>.</b> Error bars represent the standard deviation. * Significantly different (P<0.05) from the positive control.</p

Comparison of the microwave assisted methods with other physical methods and summary of their morphological size.

<p>Comparison of the microwave assisted methods with other physical methods and summary of their morphological size.</p

Fourier transform infrared spectra.

<p>(A) CMC—(a) Pure CMC and (b) CMC@AgNPs. (B) SA—(a) Pure SA and (b) SA@AgNPs.</p

Scanning electron microscopic images.

<p>(A) CMC@AgNPs and (B) SA@AgNPs.</p