Analysis of Red Complex Bacteria in the microbiome of whole Saliva in Periodontal Health and Periodontitis Individuals using Next Generation Sequencing Technology

BACKGROUND: The red complex bacteria which includes Porphyromonas gingivalis, and Tannerella forsythia (formerly Bacteroides forsythus), Treponema denticola are described as climax colonizers and are thought to be play an important pathogen in progression of periodontal disease. These bacterial species are usually found together in periodontal pockets, suggesting that they may cause destruction of the periodontal tissue in a cooperative manner. Technological developments in sequencing and identifying DNA and powerful bioinformatics tools have helped in characterization of microbia of interest in relation to the total microbial load. AIM OF THE STUDY: The aim of our study was to evaluate the presence of the red complex bacteria in saliva using Next Generation Sequencing Technology in periodontal health and periodontitis. MATERIALS AND METHODS: A total of 20 individuals seeking dental treatment in Ragas Dental College and Hospitals, Chennai, were involved in the present study, of which 10 were periodontally healthy individuals (control group) and 10 were periodontitis patients (test group). The salivary red complex was investigated with NGS technology using Illumina MiSeq sequencing method. Amplicons from V3-V4 hypervariable regions of 16S rRNA gene were sequenced. RESULTS: The results of our study suggest that the Red complex bacteria were significantly higher in periodontitis [P. gingivalis

Seaweed extract as a biostimulant for legume crop, green gram

The aim of this research is to investigate the effect of seaweed extracts obtained from the marine green algae, Ulva lactuca Linnaeus, Caulerpa scalpelliformis (R. Brown ex Turner) C. Agardh, brown algae Sargassum plagiophyllum C. Agardh, Turbinaria conoides (J. Agardh) Kutzing, Padina tetrastromatica Hauck, Dictyota dichotama (Hudson) J. V. Lamouroux on the stimulate germination, growth parameters of the Vigna radiata. The present study reveals the seeds germination, fresh weight and dry weight of shoots and roots. These results suggested that seaweed extracts stronger induce seed germination and growth parameters

Synthesis, characterization and photocatalytic application of ZnWO4/ZrO2 nanocomposite towards degradation of methyl orange dye

Visible light active ZnWO4/ZrO2 nanocomposite was prepared via hydrothermal method. The nanocomposite was characterized by UV-visible diffuse reflectance spectroscopy (UV-vis-DRS), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Scanning Electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX) and transmission electron microscopy (TEM) techniques. The XRD results showed that average particle size of ZrO2, ZnWO4 and ZnWO4/ZrO2 were found to be 29.20 nm, 23.78 nm and 20.14 nm respectively and the phase structure for ZrO2 and ZnWO4 in the composite was Rhombohedral and Monoclinic respectively. The UV–vis absorption spectra of the ZnWO4/ZrO2 nanocomposite noticeably shifted to the visible light region compared to that of the ZrO2. The prepared photocatalyst were composed of plate and spongy sphere with little agglomeration was seen from SEM result. The photocatalytic activities of the prepared nanocomposite was evaluated for the degradation of methyl orange (MO) under visible light irradiations. The effect of operational parameters such as initial dye concentration, pH, catalyst concentration and irradiation time have been investigated in detail. The photocatalytic degradation efficiency of ZnWO4/ZrO2, ZnWO4 and ZrO2 for 95%, 72% and 60 % respevtively. The high photocatalytic activity can be attributed to stronger absorption in the visible light region, a greater specific surface area, smaller crystal sizes, more surface OH groups, and to the effect of ZnWO4 doping, which resulted in a lower band gap energy

Analysis of Protective Film Formed on Carbon Steel Immersed in Seawater by Diethylenetriamine Penta (methylene phosphonic acid) -Zn 2+ System

Abstract: The inhibition efficiency of diethylenetriamine penta (methylene phosphonic acid) (DTPMP) -Zn 2+ system in controlling corrosion of carbon steel in natural sea water has been evaluated by weight-loss and electrochemical method. The formulation consisting of 250 ppm of DTPMP and 50 ppm of Zn 2+ has 98% IE. Polarization study reveals that DTPMP-Zn 2+ formulation functions as a mixed inhibitor controlling the anodic reaction and cathodic reaction to the same extent. The protective film has been analyzed by FTIR and the surface morphology of the metal surface has been analyzed by an atomic force microscopy

Influence of a paint coating on the corrosion of hull plates made of mild steel in natural seawater

The corrosion resistance of mild steel (used to make hull plates in ship technology) in seawater before paint coating (Nippon paint, weatherbond advance) and after paint coating has been measured by electrochemical studies such as Polarisation study and AC impedance spectra. It is observed that after paint coating, the corrosion resistance of mild steel hull plates increases. Polarization study reveals that after paint coating, the linear polarization resistance increases and corrosion current decreases. AC impedance spectra reveal that in the presence of paint coating charge transfer resistance value increases, impedance value increases, phase angle increases and double layer capacitance value decreases. © 2022 Authors

Identification of coronary artery stenosis based on hybrid segmentation and feature fusion

Coronary artery disease has been the utmost mutual heart disease in the past decades. Various research is going on to prevent this disease. Obstructive CAD occurs when one or more of the coronary arteries which supply blood to myocardium are narrowed owing to plaque build-up on the arteries’ inner walls, causing stenosis. The fundamental task required for the interpretation of coronary angiography is identification and quantification of severity of stenosis within the coronary circulation. Medical experts use X-ray coronary angiography to identify blood vessel/artery stenosis. Due to the artefact, the image has less clarity and it will be challenging for the medical expert to find the stenosis in the coronary artery. The solution to the problem a computational framework is proposed to segment the artery and spot the location of stenosis in the artery. Here the author presented an automatic method to detect stenosis from the X-ray angiogram image. A unified Computational method of Jerman, Level-set, fine-tuning the artery structure, is developed to extract the segmented artery features and detect the artery’s stenosis. The current experimental outcomes illustrate that this computational method achieves average specificity, sensitivity, Accuracy, precision and F-scores of 95%, 97.5%, 98%, 97.5% and 97.5%, respectively

Predictions of confirmed and death caused by COVID-19 in India

COVID-19 is spreading within the sort of a massive epidemic all over the world. This epidemic affects a lot of individuals in India. The World Health Organization states that COVID-19 could be spread from one person to another at a rapid manner through contact and respiratory spray. On these days, India and all countries worldwide should rise to an effective step to investigate this disease and eliminate the effects of this epidemic. The proposed work presents about the detailed forecasting model and prediction of the number of confirmed, recovered, and death cases in India caused by COVID-19 using machine learning algorithms. The multiple linear regressions and correlation coefficients have been applied for prediction and auto-correlation and auto-regression have been used to improve the accuracy

PORTUGALIAE ELECTROCHIMICA ACTA Inhibition of Corrosion of Carbon Steel in Sea Water by Sodium Gluconate -Zn 2+ System

Abstract The inhibition efficiency of sodium gluconate (SG)-Zn 2+ system in controlling corrosion of carbon steel in sea water has been evaluated by weight-loss method. The formulation consisting of 250 ppm of SG and 75 ppm of Zn 2+ has 98% IE. Influence of duration of immersion on the IE of SG-Zn 2+ has been evaluated. The mechanistic aspects of corrosion inhibition have been investigated by polarization study and AC impedance spectra. The protective film has been analysed by FTIR and luminescence spectra. The surface morphology and the roughness of the metal surface have been analysed by atomic force microscopy. The protective film consists of Fe 2+ -SG complex and Zn(OH) 2 . It is found to be UV -fluorescent