HLA-G: expression in human keratinocytes in vitro and in human skin in vivo

Classical, polymorphic major histocompatibility complex class I molecules are expressed on most nucleated cells.They present peptides at the cell surface and, thus, enable the immune system to scan peptides for their antigenicity. The function of the other, nonclassical class I molecules in man is controversial. HLA-G which has been shown by transfection experiments to be expressed at the cell surface, is only transcribed in placental tissue and in the fetal eye.Therefore, a role of HLA-G in the control of rejection of the allogeneic fetus has been discussed. We found that HLA-G expression is induced in keratinocytes by culture in vitro. Three different alternative splicing products of HLA-G can be detected: a full length transcript, an mRNA lacking exon 3 and a transcript devoid of exon 3 and 4. Reverse transcription followed by polymerase chain reaction also revealed the presence of HLA-G mRNA in vivo in biopsies of either diseased or healthy skin

Spin-polarization-induced structural selectivity in Pd3X_3X and Pt3X_3X (X=3dX=3d) compounds

Spin-polarization is known to lead to important {\it magnetic} and {\it optical} effects in open-shell atoms and elemental solids, but has rarely been implicated in controlling {\it structural} selectivity in compounds and alloys. Here we show that spin-polarized electronic structure calculations are crucial for predicting the correct $T=0$ crystal structures for Pd$_3X$ and Pt$_3X$ compounds. Spin-polarization leads to (i) stabilization of the $L1_2$ structure over the $DO_{22}$ structure in Pt$_3$Cr, Pd$_3$Cr, and Pd$_3$Mn, (ii) to the stabilization of the $DO_{22}$ structure over the $L1_2$ structure in Pd$_3$Co and to (iii) ordering (rather than phase-separation) in Pt$_3$Co and Pd$_3$Cr. The results are analyzed in terms of first-principles local spin density calculations.Comment: 4 pages, REVTEX, 3 eps figures, to appear in PR

Time-Gating of Pulsed Eddy Current Signals for Defect Characterization and Discrimination in Aircraft Lap-Joints

Pulsed eddy current (PEC) nondestructive testing differs from conventional eddy current techniques in that the probe coil is excited by a pulse, rather than continuous excitation at a single frequency. Reviews of early work on pulsed eddy currents are given by Waidelich1 and by Renkin.2 Pulsed excitation causes the propagation of a highly attenuated traveling wave, which is governed by the diffusion equation.3 The diffusive propagation of the eddy current pulse results in spatial broadening and a delay, or travel time, proportional to the square of the distance traveled. It was realized in early work on pulsed eddy current systems that this time dependence offered certain advantages over conventional eddy currents.4 In the current study we demonstrate the ability of a prototype pulsed eddy current instrument, described elsewhere,5,6 to take advantage of this time dependence to discriminate flaws from such interfering signals as probe liftoff, air gaps, and fasteners

Scaffolding Discourse in Asynchronous Learning Networks

Discourse, a form of collaborative learning, is fundamentally a communications process. This in-progress study adapts Clark and Brennan’s grounding in communications principles to investigate how to “scaffold” asynchronous discourse. Scaffolding is defined as providing support for the learner at his or her level until the support is no longer needed. This paper presents early results from an experimental study measuring learning effectiveness. In the experiment, content and process scaffolding are manipulated based on pedagogic principles. A major contribution of the study is building and testing a technologymediated, discourse-centered, teaching and learning model called the Asynchronous Learning Networks Cognitive Discourse Model (ALNCDM). As discourse is one of the most widely used online methods of teaching and learning, the results of the study are expected to add to the body of knowledge on how to structure asynchronous online discourse assignments for more effective student learning

Relativistic Proton Production During the 14 July 2000 Solar Event: The Case for Multiple Source Mechanisms

Protons accelerated to relativistic energies by transient solar and interplanetary phenomena caused a ground-level cosmic ray enhancement on 14 July 2000, Bastille Day. Near-Earth spacecraft measured the proton flux directly and ground-based observatories measured the secondary responses to higher energy protons. We have modelled the arrival of these relativistic protons at Earth using a technique which deduces the spectrum, arrival direction and anisotropy of the high-energy protons that produce increased responses in neutron monitors. To investigate the acceleration processes involved we have employed theoretical shock and stochastic acceleration spectral forms in our fits to spacecraft and neutron monitor data. During the rising phase of the event (10:45 UT and 10:50 UT) we find that the spectrum between 140 MeV and 4 GeV is best fitted by a shock acceleration spectrum. In contrast, the spectrum at the peak (10:55 UT and 11:00 UT) and in the declining phase (11:40 UT) is best fitted with a stochastic acceleration spectrum. We propose that at least two acceleration processes were responsible for the production of relativistic protons during the Bastille Day solar event: (1) protons were accelerated to relativistic energies by a shock, presumably a coronal mass ejection (CME). (2) protons were also accelerated to relativistic energies by stochastic processes initiated by magnetohydrodynamic (MHD) turbulence.Comment: 38 pages, 9 figures, accepted for publication in the Astrophysical Journal, January, 200

Antiprotons at Solar Maximum

New measurements with good statistics will make it possible to observe the time variation of cosmic antiprotons at 1 AU through the approaching peak of solar activity. We report a new computation of the interstellar antiproton spectrum expected from collisions between cosmic protons and the interstellar gas. This spectrum is then used as input to a steady-state drift model of solar modulation, in order to provide predictions for the antiproton spectrum as well as the antiproton/proton ratio at 1 AU. Our model predicts a surprisingly large, rapid increase in the antiproton/proton ratio through the next solar maximum, followed by a large excursion in the ratio during the following decade.New measurements with good statistics will make it possible to observe the time variation of cosmic antiprotons at 1 AU through the approaching peak of solar activity. We report a new computation of the interstellar antiproton spectrum expected from collisions between cosmic protons and the interstellar gas. This spectrum is then used as input to a steady-state drift model of solar modulation, in order to provide predictions for the antiproton spectrum as well as the antiproton/proton ratio at 1 AU. Our model predicts a surprisingly large, rapid increase in the antiproton/proton ratio through the next solar maximum, followed by a large excursion in the ratio during the following decade

Single-Molecule Dynamics of the Calcium-Dependent Activation of Plasma-Membrane Ca2+-ATPase by Calmodulin

AbstractThe plasma membrane calcium-ATPase (PMCA) helps to control cytosolic calcium levels by pumping out excess Ca2+. PMCA is regulated by the Ca2+ signaling protein calmodulin (CaM), which stimulates PMCA activity by binding to an autoinhibitory domain of PMCA. We used single-molecule polarization methods to investigate the mechanism of regulation of the PMCA by CaM fluorescently labeled with tetramethylrhodamine. The orientational mobility of PMCA-CaM complexes was determined from the extent of modulation of single-molecule fluorescence upon excitation with a rotating polarization. At a high Ca2+ concentration, the distribution of modulation depths reveals that CaM bound to PMCA is orientationally mobile, as expected for a dissociated autoinhibitory domain of PMCA. In contrast, at a reduced Ca2+ concentration a population of PMCA-CaM complexes appears with significantly reduced orientational mobility. This population can be attributed to PMCA-CaM complexes in which the autoinhibitory domain is not dissociated, and thus the PMCA is inactive. The presence of these complexes demonstrates the inadequacy of a two-state model of Ca2+ pump activation and suggests a regulatory role for the low-mobility state of the complex. When ATP is present, only the high-mobility state is detected, revealing an altered interaction between the autoinhibitory and nucleotide-binding domains

Multiscaling of galactic cosmic ray flux

Multiscaling analysis of differential flux dissipation rate of galactic cosmic rays (Carbon nuclei) is performed in the energy ranges: 56.3-73.4 Mev/nucleon and 183.1-198.7 MeV/nucleon, using the data collected by ACE/CRIS spacecraft instrument for 2000 year. The analysis reveals strong (turbulence-like) intermittency of the flux dissipation rate for the short-term intervals: 1-30 hours. It is also found that type of the intermittency can be different in different energy ranges