Rare hemoglobin variant Hb Yaizu observed in Turkey

Objective: To determine the characteristic features of the rare hemoglobin (Hb) variant Hb Yaizu to enable laboratory diagnosis of the hemoglobin variants during screening programs. Materials and Methods: Genomic DNA was obtained from the 4 members of a family living in Denizli province, an Aegean region of Turkey. Blood cell counts, hemoglobin composition, hemoglobin electrophoresis (both alkaline and acid), HPLC analysis, DNA sequencing and beta globin gene cluster haplotypes were done. Results: Hb Yaizu carriers were apparently healthy individuals. Hb Yaizu was slightly faster than Hb S at alkaline pH, but slower than Hb S at acidic pH in hemoglobin electrophoresis. An abnormal hemoglobin peak was observed with a retention time of 4.77 min in HPLC analysis attributed to Hb Yaizu. Two members of the family were heterozygous Hb Yaizu [beta 79(EF3) Asp>Asn] confirmed by DNA sequencing. The mutation was found to be linked with the Mediterranean haplotype I [+ - - ++]. Conclusion: We have presented the details of Hb Yaizu, a rare hemoglobin variant that may be important to hemoglobinopathy screening programs, although its clinical significance is unclear. Copyright © 2008 S. Karger AG

The relationship of postoperative tramadol activity with the CYP2D6*17 genome in total knee artroplasty patients

Purpose: In our study, we examined the effect of tramadol maintenance on the VAS score in geriatric patients. We observed them until the postoperative 60th minute. We investigated the incidence of pain in patients who underwent knee arthroplasty in the study. Our aim was to examine the effect of the *17 allele of the CYP2D6 genome on postoperative tramadol activity. Materials and methods: In our study we examined 110 patients who underwent total knee arthroplasty in the Department of Orthopedics and Traumatology at our facility, along with 100 healthy individuals without complaints who served as the control group. Each patient received a 100 mg dose of intravenous tramadol (Contramal). The postoperative VAS scores of the patients were recorded at 0-15-30-45-60 minutes. Results: The average age of the patients was 62.36 years. In our study, 86.4% of the patients were female, while this rate was 46% in the control group. We found that 3.65% of individuals (*17 carriers) possessed the *17 allele in both the patient group (n=7) and the control group (n=7). At the postoperative 0th minute, the VAS score for patients in the *1/*1 group was 91.07, while for the *1/*17 group, it measured 95.0. There was no statistically significant difference between the genomes (p>0.050). Likewise, no statistically significant difference was found between the genomes at the postoperative 15th, 30th, 45th, and 60th minutes (p>0.050). However, we observed a statistically significant decrease in the postoperative VAS score between 0-60 minutes in both groups, indicating time-dependent variation (p=0.000). Conclusion: When examining diverse literature on tramadol classification as intermediate metabolizer (IM) or extensive metabolizer (EM) concerning the *17 allele, our study indicates that the *17 allele should be regarded as both extensive metabolizer (EM) and normal metabolizer (NM). © 2024, Pamukkale University. All rights reserved

First observation of Hb D-Ouled rabah [beta19(B1)Asn>Lys] in the Turkish population

Hb D-Ouled Rabah [beta19(B1)Asn>Lys] is a rare hemoglobin (Hb) beta chain variant reported from Tuareg tribes in Algeria and once from China. It was suggested that Hb D-Ouled Rabah might be specific of Berber-speaking populations. Our report describes the first observation of this hemoglobin variant in the Turkish population. © Turkish Society of Hematology

Interaction of a new copper(II) complex by bovine serum albumin and dipeptidyl peptidase-IV

Dipeptidyl peptidase-IV (DPP-IV) is one of the mammalian serine proteases participated in the pathogenesis of diseases and DPP-IV inhibitors are now widely used as antidiabetic drugs. A new water soluble ternary copper (II) complex,-[Cu(py-phen) (phe) (H2O)]NO3·H2O-(py-phen:pyrazino[2,3f][1,10]phenanthroline, phe:phenylalanine), has been synthesized and characterized by CHN analysis, ESI-MS, FTIR and single-crystal X-ray diffraction techniques. Fluorescence spectroscopy was researched to study the interaction between the complex and bovine serum albumin (BSA) and dipeptidyl peptidase-IV (DPP-IV). Chromophore of BSA and DPP-IV enzyme is changed upon addition of the complex. Additionally, the complex was shown to have promising inhibitory activities against DPP-IV with lower IC50 value. This study may provide new insights into the development of effective agents against diabetes. © 2018 Elsevier B.V

Molecular studies on the origin of the Hb G-coushatta mutation in denizli province of Turkey

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