7 research outputs found
Receiver operating characteristics curves for colorectal cancer.
<p>Note. A) ROC curve for all stage CRC with a non-optimism corrected AUC of 0.8870. B) ROC curve for stage I and II CRC with a non-optimism corrected AUC of 0.8775. Receiver operating characteristics (ROC). Colorectal cancer (CRC). Area under the receiver operating characteristics curve (AUC).</p
Hypermethylated DNA, a circulating biomarker for colorectal cancer detection
<div><p>Background</p><p>Colorectal cancer (CRC) is one of the most common cancers in the western world. Screening is an efficient method of reducing cancer-related mortality. Molecular biomarkers for cancer in general and CRC in particular have been proposed, and hypermethylated DNA from stool or blood samples are already implemented as biomarkers for CRC screening.</p><p>We aimed to evaluate the performance of proven hypermethylated DNA promoter regions as plasma based biomarkers for CRC detection.</p><p>Methods</p><p>We conducted a cross-sectional case-control study of 193 CRC patients and 102 colonoscopy-verified healthy controls. Using methylation specific polymerase chain reaction, we evaluated 30 DNA promoter regions previously found to be CRC specific. We used multivariable logistic regression with stepwise backwards selection, and subsequent <i>leave-pair-out cross validation</i>, to calculate the optimism corrected area under the receiver operating characteristics curve (AUC) for all stage as well as early stage CRC.</p><p>Results</p><p>None of the individual DNA promoter regions provided an overall sensitivity above 30% at a reasonable specificity. However, seven hypermethylated promoter regions (<i>ALX4</i>, <i>BMP3</i>, <i>NPTX2</i>, <i>RARB</i>, <i>SDC2</i>, <i>SEPT9</i>, and <i>VIM</i>) along with the covariates sex and age yielded an optimism corrected AUC of 0.86 for all stage CRC and 0.85 for early stage CRC. Overall sensitivity for CRC detection was 90.7% at 72.5% specificity using a cut point value of 0.5.</p><p>Conclusions</p><p>Individual hypermethylated DNA promoter regions have limited value as CRC screening markers. However, a panel of seven hypermethylated promoter regions show great promise as a model for CRC detection.</p></div
Model 12s sensitivity, specificity, PPV, and NPV for CRC.
<p>Model 12s sensitivity, specificity, PPV, and NPV for CRC.</p
Stepwise backwards selection according to model number.
<p>Note. Logistic regression modelling with stepwise backwards selection. Potential predictor variables are located in the top row. Model number is recorded in the left column. Area under the receiver operating curve (AUC) according to model number and the P-value according to the removed predictor variable is located in the two rightward columns.</p
Number of methylated promoter regions according to patient group.
<p>Number of methylated promoter regions according to patient group.</p
Hypermethylation status according to gene names.
<p>Hypermethylation status according to gene names.</p