PCR-based landmark unique gene (PLUG) markers effectively assign homoeologous wheat genes to A, B and D genomes-0

<p><b>Copyright information:</b></p><p>Taken from "PCR-based landmark unique gene (PLUG) markers effectively assign homoeologous wheat genes to A, B and D genomes"</p><p>http://www.biomedcentral.com/1471-2164/8/135</p><p>BMC Genomics 2007;8():135-135.</p><p>Published online 30 May 2007</p><p>PMCID:PMC1904201.</p><p></p>s database [34] and wheat UniGene data sets [43] in an interactive manner. To eliminate paralogous genes, Landmark Unique Gene loci (LUGs) were selected by pair-wise comparisons of the rice cDNA models [34]. TaEST-LUGs were selected as template loci for potential PLUG markers (see Methods). "Html1" and "Html2" are interactive interfaces where the target locus can be selected and primer picking conditions can be inputted, respectively

PCR-based landmark unique gene (PLUG) markers effectively assign homoeologous wheat genes to A, B and D genomes-2

<p><b>Copyright information:</b></p><p>Taken from "PCR-based landmark unique gene (PLUG) markers effectively assign homoeologous wheat genes to A, B and D genomes"</p><p>http://www.biomedcentral.com/1471-2164/8/135</p><p>BMC Genomics 2007;8():135-135.</p><p>Published online 30 May 2007</p><p>PMCID:PMC1904201.</p><p></p>correspond to marker numbers indicated in Table 1. M: 2-Log DNA Ladder (New England BioLabs Inc., Ipswich, MA, USA)

PCR-based landmark unique gene (PLUG) markers effectively assign homoeologous wheat genes to A, B and D genomes-3

<p><b>Copyright information:</b></p><p>Taken from "PCR-based landmark unique gene (PLUG) markers effectively assign homoeologous wheat genes to A, B and D genomes"</p><p>http://www.biomedcentral.com/1471-2164/8/135</p><p>BMC Genomics 2007;8():135-135.</p><p>Published online 30 May 2007</p><p>PMCID:PMC1904201.</p><p></p>somic lines. A-C: 1% agarose gel electrophoresis of the PCR products of Marker No. 12 (A), Marker No. 18 (B), and Marker No. 8 (C). D and E: 4% agarose gel electrophoresis of the I-digested products of Marker No. 18 (D) and III-digested products of Marker No. 8 (E)

PCR-based landmark unique gene (PLUG) markers effectively assign homoeologous wheat genes to A, B and D genomes-5

<p><b>Copyright information:</b></p><p>Taken from "PCR-based landmark unique gene (PLUG) markers effectively assign homoeologous wheat genes to A, B and D genomes"</p><p>http://www.biomedcentral.com/1471-2164/8/135</p><p>BMC Genomics 2007;8():135-135.</p><p>Published online 30 May 2007</p><p>PMCID:PMC1904201.</p><p></p>s database [34] and wheat UniGene data sets [43] in an interactive manner. To eliminate paralogous genes, Landmark Unique Gene loci (LUGs) were selected by pair-wise comparisons of the rice cDNA models [34]. TaEST-LUGs were selected as template loci for potential PLUG markers (see Methods). "Html1" and "Html2" are interactive interfaces where the target locus can be selected and primer picking conditions can be inputted, respectively

PCR-based landmark unique gene (PLUG) markers effectively assign homoeologous wheat genes to A, B and D genomes-6

<p><b>Copyright information:</b></p><p>Taken from "PCR-based landmark unique gene (PLUG) markers effectively assign homoeologous wheat genes to A, B and D genomes"</p><p>http://www.biomedcentral.com/1471-2164/8/135</p><p>BMC Genomics 2007;8():135-135.</p><p>Published online 30 May 2007</p><p>PMCID:PMC1904201.</p><p></p>, as reported by Gale and Devos (1998) [5] and Sorrells et al. (2003) [35], is shown in different colors for each rice chromosome and wheat chromosome group

PCR-based landmark unique gene (PLUG) markers effectively assign homoeologous wheat genes to A, B and D genomes-1

<p><b>Copyright information:</b></p><p>Taken from "PCR-based landmark unique gene (PLUG) markers effectively assign homoeologous wheat genes to A, B and D genomes"</p><p>http://www.biomedcentral.com/1471-2164/8/135</p><p>BMC Genomics 2007;8():135-135.</p><p>Published online 30 May 2007</p><p>PMCID:PMC1904201.</p><p></p>, as reported by Gale and Devos (1998) [5] and Sorrells et al. (2003) [35], is shown in different colors for each rice chromosome and wheat chromosome group